Polynucleotides and polypeptides, materials incorporating them and methods for using them

ABSTRACT

Novel polynucleotides isolated from  Lactobacillus rhamnosus,  as well as probes and primers, genetic constructs comprising the polynucleotides, biological materials, including plants, microorganisms and multicellular organisms incorporating the polynucleotides, polypeptides expressed by the polynucleotides, and methods for using the polynucleotides and polypeptides are disclosed.

CROSS-REFERENCE TO RELATED APPLICATIONS

[0001] This application is a continuation of U.S. patent applicationSer. No. 09/724,623, filed Nov. 28, 2000, which claims to priority under35 U.S.C. §119(e) to U.S. Provisional Patent Application No. 60/148,801,filed Dec. 2, 1999.

TECHNICAL FIELD OF THE INVENTION

[0002] This invention relates to polynucleotides isolated from lacticacid bacteria, including partial and extended sequences, as well as toprobes and primers specific to the polynucleotides; DNA constructscomprising the polynucleotides; biological materials, including plants,microorganisms and multicellular organisms, incorporating thepolynucleotides; polypeptides expressed by the polynucleotides; andmethods for using the polynucleotides and polypeptides.

BACKGROUND OF THE INVENTION

[0003] The present invention relates to polynucleotides isolated from aspecific strain of lactic acid bacteria, namely Lactobacillus rhamnosusHN001 (L. rhamnosus HN001). Lactic acid bacteria, and their enzymes, arethe major determinants of flavor and fermentation characteristics infermented dairy products, such as cheese and yogurt. Flavors areproduced through the action of bacteria and their enzymes on proteins,carbohydrates and lipids.

[0004]Lactobacillus rhamnosus strain HN001 are heterofermentativebacteria that are Gram positive, non-motile, non-spore forming, catalasenegative, facultative anaerobic rods exhibiting an optimal growthtemperature of 37±1° C. and an optimum pH of 6.0- 6.5. Experimentalstudies demonstrated that dietary supplementation with Lactobacillusrhamnosus strain HN001 induced a sustained enhancement in severalaspects of both natural and acquired immunity (See PCT InternationalPublication No. WO 99/10476). In addition, L. rhamnosus HN001, andcertain other Gram-positive bacteria can specifically and directlymodulate human and animal health (See, for example, Tannock et al.,Applied Environ. Microbiol. 66:2578-2588, 2000; Gill et al., Brit. JNutrition 83:167-176; Quan Shu et al., Food and Chem. Toxicol.38:153-161, 2000; Quan Shu et al., Intl. J. Food Microbiol. 56:87-96,2000; Quan Shu et al., Intl. Dairy J. 9:831-836, 1999; Prasad et al.,Intl. Dairy J. 8:993-1002, 1998; Sanders and Huis in't Veld, Antonie vanLeeuwenhoek 76:293-315, 1999; Salminen et al., 1998. In: Lactic AcidBacteria, Salminen S and von Wright A (eds)., Marcel Dekker Inc, NewYork, Basel, Hong Kong, pp. 211-253; Delcour et al., Antonie vanLeeuwenhoek 76:159-184, 1999; Blum et al., Antonie van Leeuwenhoek76:199-205, 1999; Yasui et al., Antonie van Leeuwenhoek 76:383-389,1999; Hirayama and Rafter, Antonie van Leeuwenhoek 76:391-394, 1999;Ouwehand, 1998. In: Lactic Acid Bacteria, Salminen S and von Wright A(eds)., Marcel Dekker Inc, New York, Basel, Hong Kong, pp. 139-159;Isolauri et al., S 1998. In: Lactic Acid Bacteria, Salminen S and vonWright A (eds)., Marcel Dekker Inc, New York, Basel, Hong Kong, pp.255-268; Lichtenstein and Goldin, 1998. In: Lactic Acid Bacteria,Salminen S and von Wright A (eds)., Marcel Dekker Inc, New York, Basel,Hong Kong, pp. 269-277; El-Nezami and Ahokas, 1998. In: Lactic AcidBacteria, Salminen S and von Wright A (eds)., Marcel Dekker Inc, NewYork, Basel, Hong Kong, pp. 629-367; Nousianen et al., 1998. In: LacticAcid Bacteria, Salminen S and von Wright A (eds)., Marcel Dekker Inc,New York, Basel, Hong Kong, pp. 437-473; Meisel and Bockelmann, Antonievan Leeuwenhoek 76:207-215, 1999; Christensen et al., Antonie vanLeeuwenhoek 76:217-246, 1999; Dunne et al., Antonie van Leeuwenhoek76:279-292, 1999). Beneficial health effects attributed to thesebacteria include the following:

[0005] Increased resistance to enteric pathogens and anti-infectionactivity, including treatment of rotavirus infection and infantilediarrhea—due to increases in antibody production caused by an adjuvanteffect, increased resistance to pathogen colonization, alteration ofintestinal conditions, such as pH; and the presence of specificantibacterial substances, such as bacteriocins and organic acids.

[0006] Aid in lactose digestion—due to lactose degradation by bacteriallactase enzymes (such as beta-galactosidase) that act in the smallintestine.

[0007] Anti-cancer (in particular anti-colon cancer) andanti-mutagenesis activities—due to anti-mutagenic activity; alterationof procancerous enzymatic activity of colonic microbes; reduction of thecarcinogenic enzymes azoreductase, beta-glucuronidase and nitroreductasein the gut and/or faeces; stimulation of immune function; positiveinfluence on bile salt concentration; and antioxidant effects.

[0008] Liver cancer reduction—due to aflatoxin detoxification andinhibition of mould growth.

[0009] Reduction of small bowel bacterial overgrowth—due toantibacterial activity; and decrease in toxic metabolite production fromovergrowth flora.

[0010] Immune system modulation and treatment of autoimmune disordersand allergies—due to enhancement of non-specific and antigen-specificdefence against infection and tumors; enhanced mucosal immunity;adjuvant effect in antigen-specific immune responses; and regulation ofTh1/Th2 cells and production of cytokines.

[0011] Treatment of allergic responses to foods—due to prevention ofantigen translocation into blood stream and modulation of allergenicfactors in food.

[0012] Reduction of blood lipids and prevention of heart disease—due toassimilation of cholesterol by bacteria; hydrolysis of bile salts; andantioxidative effects.

[0013] Antihypertensive effect—bacterial protease or peptidase action onmilk peptides produces antihypertensive peptides. Cell wall componentsact as ACE inhibitors

[0014] Prevention and treatment of urogenital infections—due to adhesionto urinary and vaginal tract cells resulting in competitive exclusion;and production of antibacterial substances (acids, hydrogen peroxide andbiosurfactants).

[0015] Treatment of inflammatory bowel disorder and irritable bowelsyndrome—due to immuno-modulation; increased resistance to pathogencolonization; alteration of intestinal conditions such as pH; productionof specific antibacterial substances such as bacteriocins, organic acidsand hydrogen peroxide and biosurfactants; and competitive exclusion.

[0016] Modulation of infective endocarditis—due to fibronectinreceptor-mediated platelet aggregation associated with Lactobacillussepsis.

[0017] Prevention and treatment of Helicobacter pylori infection—due tocompetitive colonization and antibacterial effect.

[0018] Prevention and treatment of hepatic encephalopathy—due toinhibition and/or exclusion of urease-producing gut flora.

[0019] Improved protein and carbohydrate utilization and conversion—dueto production of beneficial products by bacterial action on proteins andcarbohydrates.

[0020] Other beneficial health effects associated with L. rhamnosusinclude: improved nutrition; regulation of colonocyte proliferation anddifferentiation; improved lignan and isoflavone metabolism; reducedmucosal permeability; detoxification of carcinogens and other harmfulcompounds; relief of constipation and diarrhea; and vitamin synthesis,in particular folate.

[0021] Peptidases are enzymes that break the peptide bonds linking theamino group of one amino acid with the carboxy group (acid group) of anadjacent amino acid in a peptide chain. The bonds are broken in ahydrolytic reaction. There is a large family of peptidase enzymes thatare defined by their specificity for the particular peptides bonds thatthey cleave (Barrett A J, Rawlings N D and Woessner J F (Eds.) 1998.Handbook of proteolytic enzymes. Academic Press, London, UK). The twomain families are exopeptidases and endopeptidases.

[0022] Exopeptidases cleave amino acids from the N- or C- terminus of apeptide chain, releasing free amino acids or short (di- andtripeptides). Different types of exopeptidases include:

[0023] Aminopeptidases—release a free amino acid from the N-terminus ofa peptide chain;

[0024] dipeptidyl-peptidase (also known asdipeptidyl-aminopeptidases)—release a dipeptide from the N-terminus of apeptide chain;

[0025] tripeptidyl-peptidases (also known astripeptidyl-aminopeptidases)—release a tripeptide from the N-terminus ofa peptide chain);

[0026] carboxypeptidases—release a free amino acid from the C-terminusof a peptide chain;

[0027] peptidyl-dipeptidase—release a dipeptide from the C-terminus of apeptide chain;

[0028] dipeptidases—release two free amino acids from a dipeptide; and

[0029] tripeptidases—release a free amino acid and a dipeptide from atripeptide.

[0030] Endopeptidases hydrolyze peptide bonds internally within apeptide and are classified on the basis of their mode of catalysis:

[0031] serine-endopeptidases—depend on serine (or threonine) as thenucleophile in the catalytic reaction;

[0032] cysteine-endopeptidases—depend on the sulphydryl group ofcysteine as the nucleophile in the catalytic reaction;

[0033] aspartic-endopeptidases—contain aspartate residues that act asligands for an activated water molecule which acts as the nucleophile inthe catalytic reaction; and

[0034] metallo-endopeptidases—contain one or more divalent metal ionsthat activate the water molecule that acts as the nucleophile in thecatalytic reaction.

[0035] Peptidases are important enzymes in the process of cheeseripening and the development of cheese flavor. The hydrolysis of milkcaseins in cheese results in textural changes and the development ofcheese flavors. The raft of proteolytic enzymes that cause thishydrolysis come from the lactic acid bacteria that are bound up in thecheese—either starter cultures that grow up during the manufacture ofthe cheese, or adventitious and adjunct non-starter lactic acid bacteriathat grow in the cheese as it ripens (Law Haandrikman, Int. Dairy J.7:1-11, 1997).

[0036] Many other enzymes can also influence dairy product flavor, andfunctional and textural characteristics, as well as influencing thefermentation characteristics of the bacteria, such as speed of growth,acid production and survival (Urbach, Int. Dairy J. 5:877-890, 1995;Johnson and Somkuti, Biotech. Appl. Biochem. 13:196-204, 1991; El Sodaand Pandian, J. Dairy Sci. 74:2317-2362, 1 991; Fox et al,. In Cheese:chemistry, physics and microbiology. Volume 1, General aspects, 2^(nd)edition, P Fox (ed) Chapman and Hall, London; Christensen et al.,Antonie van Leeuwenhoek 76:217-246, 1999; Stingle et al., J. Bacteriol.20:6624-6360, 1999; Stingle et al., Mol. Microbiol. 32:1287-1295, 1999;Lemoine et al., Appl. Environ. Microbiol. 63:1512-6218, 1997). Enzymesinfluencing specific characteristics and/or functions include thefollowing:

[0037] Lysis of cells. These enzymes are mostly cell wall hydrolases,including amidases; muramidases; lysozymes, including N-acetylmuramidase; muramidase; N-acetylglucosaminidase; andN-acetylmuramoyl-L-alanine amidase. DEAD-box helicase proteins alsoinfluence autolysis.

[0038] Carbohydrate utilization. Lactose, citrate and diacetylmetabolism, and alcohol metabolism are particularly important. Theenzymes involved include beta-galactosidase, lactate dehydrogenase,citrate lyase, citrate permease, 2,3 butanediol dehydrogenase (acetoinreductase), acetolactate decaboxylase, acetolactate synthase, pyruvatedecarboxylase, pyruvate formate lyase, diacetyl synthase, diacetylreductase, alcohol decarboxylase, lactate dehydrogenase, pyruvatedehydrogenase, and aldehyde dehydrogenase.

[0039] Lipid degradation, modification or synthesis. Enzymes involvedinclude lipases, esterases, phospholipases, serine hydrolases,desaturases, and linoleate isomerase.

[0040] Polysaccharide synthesis. Polysaccharides are important not onlyfor potential immune enhancement and adhesion activity but are importantfor the texture of fermented dairy products. The enzymes involved are aseries of glucosyl transferases, including beta-(1-3) glucosyltransferase, alpha-N acetylgalactosaminyl transferase, phosphogalactosyltransferase, alpha-glycosyl transferase, UDP-N-acetylglucosamine C4epimerase and UDP-N-acetylglucosamine transferase.

[0041] Amino acid degradation. Enzymes include glutamate dehydrogenase,aminotransferases, amino acid decarboxylases, and enzymes involved insulphur amino acid degradation including cystothione beta-lyase.

[0042] Sequencing of the genomes, or portions of the genomes, ofnumerous organisms, including humans, animals, microorganisms andvarious plant varieties, has been and is being carried out on a largescale. Polynucleotides identified using sequencing techniques may bepartial or full-length genes, and may contain open reading frames, orportions of open reading frames, that encode polypeptides. Putativepolypeptides may be identified based on polynucleotide sequences andfurther characterized. The sequencing data relating to polynucleotidesthus represents valuable and useful information.

[0043] Polynucleotides and polypeptides may be analyzed for varyingdegrees of novelty by comparing identified sequences to sequencespublished in various public domain databases, such as EMBL. Newlyidentified polynucleotides and corresponding putative polypeptides mayalso be compared to polynucleotides and polypeptides contained in publicdomain information to ascertain homology to known polynucleotides andpolypeptides. In this way, the degree of similarity, identity orhomology of polynucleotides and polypeptides having an unknown functionmay be determined relative to polynucleotides and polypeptides havingknown functions.

[0044] Information relating to the sequences of isolated polynucleotidesmay be used in a variety of ways. Specified polynucleotides having aparticular sequence may be isolated, or synthesized, for use in in vivoor in vitro experimentation as probes or primers. Alternatively,collections of sequences of isolated polynucleotides may be stored usingmagnetic or optical storage medium and analyzed or manipulated usingcomputer hardware and software, as well as other types of tools.

SUMMARY OF THE INVENTION

[0045] The present invention provides isolated polynucleotidescomprising a sequence selected from the group consisting of: (a)sequences identified in the attached Sequence Listing as SEQ ID NOS:1-62; (b) variants of those sequences; (c) extended sequences comprisingthe sequences set out in SEQ ID NOS: 1-62, and their variants; and (d)sequences comprising at least a specified number of contiguous residuesof a sequence of SEQ ID NOS: 1-62 (x-mers). Oligonucleotide probes andprimers corresponding to the sequences set out in SEQ ID NOS: 1-62, andtheir variants are also provided. All of these polynucleotides andoligonucleotide probes and primers are collectively referred to herein,as “polynucleotides of the present invention.”

[0046] The polynucleotide sequences identified as SEQ ID NOS: 1-62 werederived from a microbial source, namely from fragmented genomic DNA ofLactobacillus rhamnosus, strain HN001, described in PCT InternationalPublication No. WO 99/10476. Lactobacillus rhamnosus strain HN001 areheterofermentative bacteria that are Gram positive, non-motile,non-spore forming, catalase negative, facultative anaerobic rodsexhibiting an optimal growth temperature of 37±1° C. and an optimum pHof 6.0-6.5. Experimental studies demonstrated that dietarysupplementation with Lactobacillus rhamnosus strain HN001 induced asustained enhancement in several aspects of both natural and acquiredimmunity. A biologically pure culture of Lactobacillus rhamnosus strainHN001 was deposited at the Australian Government Analytical Laboratories(AGAL), The New South Wales Regional Laboratory, 1 Suakin Street,Pymble, NSW 2073, Australia, as Deposit No. NM97/09514, dated Aug. 18,1997.

[0047] Certain of the polynucleotide sequences disclosed herein are“partial” sequences in that they do not represent a full-length geneencoding a full-length polypeptide. Such partial sequences may beextended by analyzing and sequencing various DNA libraries using primersand/or probes and well-known hybridization and/or PCR techniques. Thepartial sequences disclosed herein may thus be extended until an openreading frame encoding a polypeptide, a full-length polynucleotideand/or gene capable of expressing a polypeptide, or another usefulportion of the genome is identified. Such extended sequences, includingfull-length polynucleotides and genes, are described as “correspondingto” a sequence identified as one of the sequences of SEQ ID NOS: 1-62 ora variant thereof, or a portion of one of the sequences of SEQ ID NOS:1-62 or a variant thereof, when the extended polynucleotide comprises anidentified sequence or its variant, or an identified contiguous portion(x-mer) of one of the sequences of SEQ ID NOS: 1-62 or a variantthereof.

[0048] The polynucleotides identified as SEQ ID NOS: 1-62 were isolatedfrom Lactobacillus rhamnosus genomic DNA clones and represent sequencesthat are present in the cells from which the DNA was prepared. Thesequence information may be used to identify and isolate, or synthesize,DNA molecules such as promoters, DNA-binding elements, open readingframes or full-length genes, that then can be used as expressible orotherwise functional DNA in transgenic organisms. Similarly, RNAsequences, reverse sequences, complementary sequences, antisensesequences and the like, corresponding to the polynucleotides of thepresent invention, may be routinely ascertained and obtained using thepolynucleotides identified as SEQ ID NOS: 1-62.

[0049] The present invention further provides isolated polypeptidesencoded, or partially encoded, by the polynucleotides disclosed herein.In certain specific embodiments, the polypeptides of the presentinvention comprise a sequence selected from the group consisting ofsequences identified as SEQ ID NO: 63-124, and variants thereof.Polypeptides encoded by the polynucleotides of the present invention maybe expressed and used in various assays to determine their biologicalactivity. Such polypeptides may be used to raise antibodies, to isolatecorresponding interacting proteins or other compounds, and toquantitatively determine levels of interacting proteins or othercompounds.

[0050] Genetic constructs comprising the inventive polynucleotides arealso provided, together with transgenic host cells comprising suchconstructs and transgenic organisms, such as microbes, comprising suchcells.

[0051] The present invention also contemplates methods for modulatingthe polynucleotide and/or polypeptide content and composition of anorganism, such methods involving stably incorporating into the genome ofthe organism a genetic construct comprising a polynucleotide of thepresent invention. In one embodiment, the target organism is a microbe,preferably a microbe used in fermentation, more preferably a microbe ofthe genus Lactobacillus, and most preferably Lactobacillus rhamnosus, orother closely microbial related species used in the dairy industry. In arelated aspect, methods for producing a microbe having an alteredgenotype and/or phenotype is provided, such methods comprisingtransforming a microbial cell with a genetic construct of the presentinvention to provide a transgenic cell, and cultivating the transgeniccell under conditions conducive to growth and multiplication. Organismshaving an altered genotype or phenotype as a result of modulation of thelevel or content of a polynucleotide or polypeptide of the presentinvention compared to a wild-type organism, as well as components andprogeny of such organisms, are contemplated by and encompassed withinthe present invention.

[0052] The isolated polynucleotides of the present invention may beusefully employed for the detection of lactic acid bacteria, preferablyL. rhamnosus, in a sample material, using techniques well known in theart, such as polymerase chain reaction (PCR) and DNA hybridization, asdetailed below.

[0053] The inventive polynucleotides and polypeptides may also beemployed in methods for the selection and production of more effectiveprobiotic bacteria; as “bioactive” (health-promoting) ingredients andhealth supplements, for immune function enhancement; for reduction ofblood lipids such as cholesterol; for production of bioactive materialfrom genetically modified bacteria; as adjuvants; for wound healing; invaccine development, particularly mucosal vaccines; as animal probioticsfor improved animal health and productivity; in selection and productionof genetically modified rumen microorganisms for improved animalnutrition and productivity, better flavor and improved milk composition;in methods for the selection and production of better natural foodbacteria for improved flavor, faster flavor development, betterfermentation characteristics, vitamin synthesis and improved texturalcharacteristics; for the production of improved food bacteria throughgenetic modification; and for the identification of novel enzymes forthe production of, for example, flavors or aroma concentrates.

[0054] The isolated polynucleotides of the present invention also haveutility in genome mapping, in physical mapping, and in positionalcloning of genes of more or less related microbes.

[0055] Additionally, the polynucleotide sequences identified as SEQ IDNOS: 1-62, and their variants, may be used to design oligonucleotideprobes and primers. Oligonucleotide probes and primers have sequencesthat are substantially complementary to the polynucleotide of interestover a certain portion of the polynucleotide. Oligonucleotide probesdesigned using the polynucleotides of the present invention may be usedto detect the presence and examine the expression patterns of genes inany organism having sufficiently similar DNA and RNA sequences in theircells, using techniques that are well known in the art, such as slotblot DNA hybridization techniques. Oligonucleotide primers designedusing the polynucleotides of the present invention may be used for PCRamplifications. Oligonucleotide probes and primers designed using thepolynucleotides of the present invention may also be used in connectionwith various microarray technologies, including the microarraytechnology of Affymetrix (Santa Clara, Calif.).

[0056] The polynucleotides of the present invention may also be used totag or identify an organism or derived material or product therefrom.Such tagging may be accomplished, for example, by stably introducing anon-disruptive non-functional heterologous polynucleotide identifierinto an organism, the polynucleotide comprising at least a portion of apolynucleotide of the present invention.

[0057] The polynucleotides of the present invention may additionally beused as promoters, gene regulators, origins of DNA replication,secretion signals, cell wall or membrane anchors for genetic tools (suchas expression or integration vectors).

[0058] All references cited herein, including patent references andnon-patent publications, are hereby incorporated by reference in theirentireties.

DETAILED DESCRIPTION

[0059] The polynucleotides disclosed herein were isolated by highthroughput sequencing of DNA libraries from the lactic acid bacteriaLactobacillus rhamnosus as described in Example 1. Cell wall, cellsurface and secreted components of lactic acid bacteria are known tomediate immune modulation, cell adhesion and antibacterial activities,resulting in many beneficial effects including: resistance to entericpathogens; modulation of cancer, including colon cancer:anti-mutagenesis effects; reduction of small bowel bacterial overgrowth;modulation of auto-immune disorders; reduction in allergic disorders;modulation of urogenital infections, inflammatory bowel disorder,irritable bowel syndrome, Helicobacter pylori infection and hepaticencephalopathy; reduction of infection with pathogens; regulation ofcolonocyte proliferation and differentiation; reduction of mucosalpermeability; and relief of constipation and diarrhea. These cellcomponents include, but are not limited to, peptidoglycans, teichoicacids, lipoteichoic acids, polysaccharides, adhesion proteins, secretedproteins, surface layer or S-layer proteins, collagen binding proteinsand other cell surface proteins, and antibacterial substances such asbacteriocins and organic acids produced by these bacteria.Polynucleotides involved in the synthesis of these proteins and in thesynthesis, modification, regulation, transport, synthesis and/oraccumulation of precursor molecules for these proteins can be used tomodulate the immune, antibacterial, cell adhesion and competitiveexclusion effects of the bacteria or of components that might beproduced by these bacteria.

[0060] In order to function effectively as probiotic bacteria, L.rhamnosus HN001 must survive environmental stress conditions in thegastrointestinal tract, as well as commercial and industrial processes.Modification of particular polynucleotides or regulatory processes havebeen shown to be effective against a number of stresses includingoxidative stress, pH, osmotic stress, dehydration, carbon starvation,phosphate starvation, nitrogen starvation, amino acid starvation, heator cold shock, and mutagenic stress. Polynucleotides involved in stressresistance often confer multistress resistance, i.e., when exposed toone stress, surviving cells are resistant to several non-relatedstresses. Bacterial genes and/or processes shown to be involved inmultistress resistance include:

[0061] Intracellular phosphate pools—inorganic phosphate starvationleads to the induction of pho regulon genes, and is linked to thebacterial stringent response. Gene knockouts involving phosphatereceptor genes appear to lead to multistress resistance.

[0062] Intracellular guanosine pools—purine biosynthesis and scavengerpathways involve the production of phosphate-guanosine compounds thatact as signal molecules in the bacterial stringent response. Geneknockouts involving purine scavenger pathway genes appear to confermultistress resistance.

[0063] Osmoregulatory molecules—small choline-based molecules, such asglycine-betaine, and sugars, such as trehalose, are protective againstosmotic shock and are rapidly imported and/or synthesized in response toincreasing osmolarity.

[0064] Acid resistance—lactobacilli naturally acidify their environmentthrough the excretion of lactic acid, mainly through the cit operongenes responsible for citrate uptake and utilization.

[0065] Stress response genes—a number of genes appear to be induced orrepressed by heat shock, cold shock, and increasing salt through theaction of specific promoters.

[0066] The isolated polynucleotides of the present invention, andgenetic constructs comprising such polynucleotides, may be employed toproduce bacteria having desired phenotypes, including increasedresistance to stress and improved fermentation properties.

[0067] Many enzymes are known to influence dairy product flavor,functional and textural characteristics as well as general fermentationcharacteristics such as speed of growth, acid production and survival.These enzymes include those involved in the metabolism of lipids,polysaccharides, amino acids and carbohydrates, as well as thoseinvolved in the lysis of the bacterial cells.

[0068] The isolated polynucleotides and polypeptides of the presentinvention have demonstrated similarity to polynucleotides and/orpolypeptides of known function. The putative identity and functions ofthe inventive polynucleotides based on such similarities are shown belowin Table 1. TABLE 1 SEQ ID NO SEQ ID NO Polynucleotide Polypeptide Genefunction or protein class 1 63 Transmembrane protein that participatesin the adhesion of bacteria to gut cells, part of an operon containingthe mapA gene encoding a mucin binding protein. This gene may be used toidentify or manipulate interactions with gut cells. 2 64 Common 28 kDaantigen and major cell adherence molecule of Campylobacter jejuni andCampylobacter coli. Significant similarity to amino acid transportproteins in Gram-negative bacteria. This gene may be used to identify ormanipulate both interactions with gut cells and amino acid metabolism. 365 Histidinol-phosphate aminotransferase, may also have tyrosine andphenylalanine aminotransferase activity. Involved in amino acidmetabolism. May be used to identify or manipulate metabolism andinfluence growth and the production of flavor compounds. 4 66 Aspartatetransaminase (EC 2.6.1.1). Converts L-aspartate and 2-oxoglutarate tooxaloacetate and L-glutamate, but may also be involved in aromatic aminoacid, alanine, cysteine, proline, and asparagine pathways. Its roleamino acid metabolism suggests impact in production of flavor compounds,and may also be involved in carbon fixation. May be used to identify ormanipulate metabolism and influence growth and the production of flavorcompounds. 5 67 Aromatic amino acid transferase. It is used to identifyor manipulate metabolism and influence growth and the production offlavor compounds.. 6 68 Tyrosine aminotransferase (EC 2.6.1.5)(L-tyrosine: 2- oxoglutarate aminotransferase). Transfers nitrogenousgroups as part of the aromatic amino acid pathway. Involved in synthesisof flavor compounds and amino acid metabolism. It is used to identify ormanipulate metabolism and influence growth and the production of flavorcompounds. 7 69 Aminotransferase B. Probable aminotransferase belongingto class-II pyridoxal-phosphate-dependent aminotransferase family. it isused to identify or manipulate metabolism and influence growth and theproduction of flavor compounds. 8 70 Cysteine desulfurase, a class-Vaminotransferase that supplies inorganic sulfide for Fe—S clusters.Involved in cysteine metabolism and generation of flavor compounds. Itis used to identify or manipulate metabolism and influence growth andthe production of flavor compounds. 9 71 Lipase, breakdown oftriglycerides. It is used to identify or manipulate metabolism andinfluence growth and the production of flavor compounds. 10 72O-acetylserine sulfhydrylase involved in cysteine synthesis. ConvertsO-acetyl-L-serine and H2S to L-cysteine and acetate. Involved insynthesis of flavor and aroma compounds. It is used to identify ormanipulate metabolism and influence growth and the production of flavorcompounds. 11 73 Surface protein thought to be involved in a number offunctions including as a collagen and/or mucin binding protein incellular adhesion and as a cysteine transporter, part of the ABCsuperfamily, which affects amino acid metabolism and flavor compoundsynthesis. It is used to identify or manipulate metabolism, growth, theproduction of flavor compounds, and interactions with gut cells. 12 74Group B streptococcal oligopeptidase, degrades a variety of bioactivepeptides. Involved in protein breakdown and metabolism, and may impacton flavor compounds as well impact on health through the stability orproduction of bioactive peptides. 13 75 Pz-peptidase, ametalloproteinase and part of the thimet oligopeptidase family.Hydrolyses the Pz-peptide, 4-phenylazobenzyloxycarbonyl-Pro-Leu-Gly-Pro-Arg. It impacts on flavorcompounds as well impact on health through the stability or productionof bioactive peptides. 14 76 Adenosine triphosphatase clpC.ATP-dependent Clp proteinase regulatory protein, a pleiotropic regulatorcontrolling growth at high temperatures. Involved in stress response. Itis used to identify or impact on the survival or virulance of organisms.15 77 Streptococcal C5a peptidase. Specifically cleaves human serumchemotaxin C5a near its C-terminus, destroying its ability to serve as achemoattractant. It mediates interactions with host immune system and isused to identify or impact on interactions with immune systems. 16 78Dipeptidase from Lactococcus lactis. Hydrolyzes a broad range ofdipeptides but no tri, tetra, or larger oligopeptides. It is used toidentify or impact on protein metabolism and flavor compound synthesis.17 79 Acylamino-acid-releasing enzyme (acyl-peptidehydrolase oracylaminoacyl-peptidase) BC 3.4.19.1. Catalyzes removal Nalpha-acetylated amino acid residues from N alpha-acetylated peptides.It is used to identify or impact on metabolism or flavor or aromacompound production. 18 80 Heat shock protease regulatory subunit, theATPase subunit of an intracellular ATP-dependent protease. It is used toidentify or impact on survival or virulence. 19 81 O-sialoglycoproteinendopeptidase (EC 3.4.24.57). Hydrolyses O-sialoglycoproteins, but doesnot cleave unglycosylated proteins, desialylated glycoproteins orN-glycosylated glycoproteins. Sialogylcoproteins can act as receptorsfor adhesion to gut cells. It is used to identify or impact oninteractions with gut cells, protein metabolism, stability or productionof bioactive peptides. 20 82 Carboxylesterase, converts a carboxylicester to an alcohol and a carboxylic acid anion. Esters and alcohols canbe potent flavor and aroma compounds. It is used to identify or impacton metabolism or flavor or aroma compound production. 21 83Glycerophosphodiester phosphodiesterase. Converts glycerophosphodiestersto an alcohol and glycerol 3-phosphate. Alcohols are potentiallyimportant flavor compounds. It is used to identify or impact onmetabolism or flavor or aroma compound production. 22 84 Bifunctionalalcohol dehydrogenase and acetaldehyde dehydrogenase. Ferments glucoseto ethanol under anaerobic conditions. It is used to identify or impacton metabolism or flavor or aroma compound production. 23 85 Short-chainalcohol dehydrogenase. It is used to identify or impact on metabolism orflavor or aroma compound production. 24 86 Aryl-alcohol dehydrogenase.Converts an aromatic alcohol to an aromatic aldehyde. It is used toidentify or impact on metabolism or flavor or aroma compound production.25 87 Branched chain amino acid transport system II carrier protein,involved in amino acid metabolism. Amino acid metabolism is important inflavor compound production. It is used to identify or impact onmetabolism or flavor compound production. 26 88 Human bile salt exportpump. Bile tolerance is an important property of probiotic bacteria.Bile salt removal can reduce cholesterol. May be used to identify orimpact on bile tolerance or cholesterol reduction. 27 89 BifunctionalHPr Kinase/P-Ser-HPr phosphatase from Lactobacillus casei. Controlscatabolite repression and involved in phosphate regulation. Phosphateregulation is important in cell survival and stress tolerance. It isused to identify or impact on gene regulation and on stress tolerance.28 90 Suppressor of dominant negative ftsH mutations affectingextracellular protein transport in E. coli. It is used to identify orimpact on protein transport. 29 91 Malolactic enzyme. Converts betweenmalate and lactate. Central to carbohydrate metabolism, also involved inacid tolerance. It is used to identify or impact on metabolism or flavorcompound production or cell survival. 30 92 Magnesium transporter, alsohas affinity for cobalt. Metal ion transport is involved in bacterialsurvival as well as other aspects of metabolism. It is used to identifyor impact on metabolism or cell survival. 31 93 Pyruvate dehydrogenaseE1 (lipoamide) alpha subunit (EC 1.2.4.1). Glycolytic enzyme, alsoinvolved in branched-chain amino acid synthesis. It is used to identifyor impact on metabolism or flavor or aroma compound production. 32 94Adhesin involved in diffuse adherence of diarrhoeagenic E. coli. May beused to identify or impact on interactions with gut cells, survival andpersistence in the gut. 33 95 dTDP-4-keto-L. rhamnose reductase involvedin polysaccharide biosynthesis. Polysaccharides are important foradhesion to gut cells, immune system modulation, stress tolerance andfor physical properties of fermented products. It is used to identify orimpact on polysaccharide production and interaction with gut cells. 3496 Glucose inhibited division protein. Involved in stress resistance,gidA mutants are UV-sensitive and exhibit decreased homologousrecombination in plasmidic tests. It is used to identify or impact oncell survival and gene regulation. 35 97 Glucose-1-phosphate thymidylyltransferase, involved in polysaccharide biosynthesis. Polysaccharidesare important for adhesion to gut cells, immune system modulation,stress tolerance and for physical properties of fermented products. Itis used to identify or impact on polysaccharide production andinteraction with gut cells. 36 98 Phosphate starvation-induced protein,may be important for survival under low phospate conditions. Phosphatelevels have been shown to be important in multistress resistance. It isused to identify or impact on cell survival. 37 99 FormateC-acetyltransferase (or pyruvate formate lyase, EC 2.3.1.54). Convertsformate to pyruvate during malate utilization. Pyruvate is central tocell metabolism. It is used to identify or impact on metabolism and thegeneration of flavor compounds. 38 100 Alpha-glycerophosphate oxidase.Oxidizes alpha- glycerophosphate to dihydroxyacetone phosphate whilereducing oxygen to hydrogen peroxide. These compounds are important formetabolism as well as antimicrobial activity. It is used to identify orimpact on metabolism and the generation of flavor compounds as well asantimicrobial activity. 39 101 6-Phosphogluconate dehydrogenase.Converts 6-phospho-D- gluconate to D-ribulose 5-phosphate and CO2, partof the hexose monophosphate shunt pathway used for carbohydratemetabolism. It is used to identify or impact on metabolism and thegeneration of flavor compounds. 40 1025-methyltetrahydropteroyltriglutamate homocysteine methyltransferase.Converts 5-methyltetrahydropteroyltri-L- glutamate and L-homocysteine toTetrahydropteroyltri-L- glutamate and L-methionine. Sulpher compoundsare important in flavor development. Homocysteine is important incardiovascular health. It is used to identify or impact on metabolismand the generation of flavor or aroma compounds as well ascardiovascular health. 41 103 S-methylmethionine permease. Integralmembrane protein involved in S-methylmethionine uptake. Sulfur compoundsare important in flavor development, and 5-methylmethionine may also beinvolved in cellular methylation pathways. Cellular methylation isimportant for gene regulation. It is used to identify or impact onmetabolism and the generation of flavor compounds and for cellularmethylation. 42 104 6-Phospho-beta-galactosidase. Central to lactosemetabolism, results in alcohol compounds that may have flavorproperties. It is used to identify or impact on metabolism and thegeneration of flavor compounds. 43 105 GTP binding protein, membranebound. Involved in the stress response. It is used to identify or impacton cell survival. 44 106 Gamma-glutamyl phosphate reductase(glutamate-5- semialdehyde dehydrogenase), involved in prolinebiosynthesis and amino acid metabolism pathways. It is used to identifyor impact on metabolism and the generation of flavor compounds. 45 107Dihydrofolate reductase (EC 1.5.1.3), responsible for resistance to thecytotoxic drug methotrexate and involved in vitamin synthesis. It isused to identify or impact on metabolism and the generation of vitamincompounds and for drug resistance. 46 108 Lactate dehydrogenase.Converts lactate to pyruvate, also has a role in acid tolerance. Lactatecan have antimicrobial effects. It is used to identify or impact onmetabolism and the generation of flavor compounds, for cell survival andvirulence and antimicrobial effects. 47 109 Heat-inducible transcriptionrepressor protein. Involved in stress resistance. It is used to identifyor impact on survival and on gene regulation. 48 110 Daunorubicinresistance protein (DrrC) is a daunorubicin resistance protein with astrong sequence similarity to the UvrA protein that is involved inexcision repair of DNA. DrrC is induced by the anticancer drugdaunorubicin and behaves like an ATP-dependent, DNA binding protein invitro. 49 111 Dihydrodipicolinate synthase (ec 4.2.1.52) (DHDPS) is alsoknown as DapA or AF0910. DapA catalyzes the first step in thebiosynthesis of diaminopimelate and lysine from aspartate semialdehyde.The known pathways for diaminopimelate (DAP) and lysine biosynthesisshare two key enzymes, dihydrodipicolinate synthase anddihydrodipicolinate reductase, encoded by the dapA and dapB genes,respectively. Diaminopimelate (DAP) is a metabolite that is alsoinvolved in peptidoglycan formation. DapA can be used for the industrialproduction of L-lysine. DHDPS belongs to the DHDPS family. 50 112 Lysin(Lys) is one of the lytic enzymes encoded bu bacteriophages. Togetherwith holin, lysis of bacteria used in cheese-making can be achieved toaccelerate cheese ripening and to facilitated release of intracellularenzymes involvement in flavor formation. Production of holin alone leadsto partial lysis of the host cells, whereas production of lysin alonedoes not cause significant lysis. Model cheese experiments in which aninducible holinlysin overproducing strain was used showed a fourfoldincrease in release of L-Lactate dehydrogenase activity into the curdrelative to the control strain and the holin- overproducing strain,demonstrating the suitability of the system for cheese applications. 51113 Penicillin-binding protein 1A or PDPF is penicillin-binding proteinPBP 1A that is an essential murein polymerases of bacteria. Thepenicillin binding proteins (PBPs) synthesize and remodel peptidoglycan,the structural component of the bacterial cell wall. Resistance tobeta-lactam antibiotics in bacteria is due to alteration of thepenicillin-binding proteins (PBPs). PBP 1A belongs to the class Ahigh-molecular-mass PBPs, which harbor transpeptidase (TP) andglycosyltransferase (GT) activities. The GT active site represents atarget for the generation of novel non-penicillin antibiotics. 52 114Virulence-associated protein BH6253 plays a role in the virulence of thepathogens. 53 115 Adherence and virulence protein A (Pav A) is avirulence factor that is widely distributed in bacteria and participatesin adherence to host cells and soft tissue pathology. 54 116 Prolineiminopeptidase gene (pepI) is part of an operon-like structure of threeopen reading frames (ORF1, ORF2 and ORF3). ORF1 was preceded by atypical prokaryotic promoter region, and a putative transcriptionterminator was found downstream of ORF3, identified as the pepI gene.PepI was shown to be a metal-independent serine peptidase having thiolgroups at or near the active site. Kinetic studies identifiedproline-p-nitroanilide as substrate. PepI is a dimer of M(r) 53,000. Theenzyme can be utilized to facilitate the accumulation of proline fromdipeptides and oligopeptides during the ripening of cheese. 55 117Sensory transduction protein regX3 forms part of a two- componentregulatory system regX3/senX3 phosphorylated by senX3. The N-terminalregion is similar to that of other regulatory components of sensorytransduction systems. The senX3-regX3 IR contains a novel type ofrepetitive sequence, called mycobacterial interspersed repetitive units(MIRUs). The regX3 gene has utility in diagnostic assays todifferentiate between bacterial strains. 56 118 Aminopeptidase pepS (ec3.4.11.-) is part of the proteolytic system of lactic acid bacteria thatis essential for bacterial growth in milk and for development of theorganoleptic properties of dairy products. PepS is a monomericmetallopeptidase of approximately 45 kDa with optimal activity in therange pH 7.5-8.5 and at 55 degrees C. on Arg- paranitroanilide assubstrate. PepS exhibits a high specificity towards peptides possessingarginine or aromatic amino acids at the N-terminus. PepS is part of theaminopeptidase T family. In view of its substrate specificity, PepS isinvolved both in bacterial growth by supplying amino acids, and in thedevelopment of dairy products' flavor, by hydrolysing bitter peptidesand liberating aromatic amino acids which are important precursors ofaroma compounds. 57 119 Phosphoribosylaminoimidazolecarboxamideformyltransferase/imp cyclohydrolase (ec 2.1.2.3) (purH) or AICARFT isbiosynthetic enzyme in the de novo purine biosynthesis pathway. 58 120Prolinase (pepR) is a peptidase gene expressing L-proline-beta-naphthylamide-hydrolyzing activity. PepR was shown to be the primaryenzyme capable of hydrolyzing Pro-Leu in Lactobacilli. The purifiedenzyme hydrolyzed Pro-Met, Thr-Leu, and Ser-Phe as well as dipeptidescontaining neutral, nonpolar amino acid residues at the amino terminus.Purified pepR was determined to have a molecular mass of 125 kDa withsubunits of 33 kDa. The isoelectric point of the enzyme was determinedto be 4.5. PepR is a serine-dependent protease that can be utilized inproduction of dairy products where it is used to acidify milk. 59 121Hexulose-6-phosphate isomerase (ec 5.-.-.-) is also known as HumpI orSGBU and is part of a sugar metabolic pathway along with sgbh where itis involved in isomerization of D-arabino-6- hexulose 3-phosphate toD-fructose 6-phosphate. SGBU belongs to the HumpI family. 60 122Succinyl-diaminopimelate desuccinylase encodes the DapE that has utilityas antibiotic target. 61 123 Transcriptional regulator (GntR family) ispart of the GntR family of DNA binding proteins that has acharacteristic helix- turn-helix motif. The motif interacts with DNAdouble helix and recognizes specific base sequences. 62 124 Xaa-Prodipeptidase (ec 3.4.13.9) is also known as X-Pro dipeptidase, prolinedipeptidase, prolidase, imidodipeptidase or pepQ. PepQ is involved inthe hydrolysis of Xaa-|-Pro dipeptides and also acts onaminoacyl-hydroxyproline analogs. PepQ belongs to peptidase family M24b.PepQ can be utilized in the production of cheese.

[0069] Isolated polynucleotides of the present invention include thepolynucleotides identified herein as SEQ ID NOS: 1-62; isolatedpolynucleotides comprising a polynucleotide sequence selected from thegroup consisting of SEQ ID NOS: 1-62; isolated polynucleotidescomprising at least a specified number of contiguous residues (x-mers)of any of the polynucleotides identified as SEQ ID NOS: 1-62; isolatedpolynucleotides comprising a polynucleotide sequence that iscomplementary to any of the above polynucleotides; isolatedpolynucleotides comprising a polynucleotide sequence that is a reversesequence or a reverse complement of any of the above polynucleotides;antisense sequences corresponding to any of the above polynucleotides;and variants of any of the above polynucleotides, as that term isdescribed in this specification.

[0070] The word “polynucleotide(s),” as used herein, means a single ordouble stranded polymer of deoxyribonucleotide or ribonucleotide basesand includes DNA and corresponding RNA molecules, including mRNAmolecules, both sense and antisense strands of DNA and RNA molecules,and comprehends cDNA, genomic DNA and recombinant DNA, as well as whollyor partially synthesized polynucleotides. A polynucleotide of thepresent invention may be an entire gene, or any portion thereof. A geneis a DNA sequence which codes for a functional protein or RNA molecule.Operable antisense polynucleotides may comprise a fragment of thecorresponding polynucleotide, and the definition of “polynucleotide”therefore includes all operable antisense fragments. Antisensepolynucleotides and techniques involving antisense polynucleotides arewell known in the art and are described, for example, inRobinson-Benion, et al., “Antisense techniques,” Methods in Enzymol.254(23): 363-375, 1995; and Kawasaki, et al., Artific. Organs 20 (8):836-848, 1996.

[0071] The definitions of the terms “complement,” “reverse complement,”and “reverse sequence,” as used herein, are best illustrated by thefollowing examples. For the sequence 5′ AGGACC 3′, the complement,reverse complement, and reverse sequences are as follows: complement3′ TCCTGG 5′ reverse complement 3′ GGTCCT 5′ reverse sequence 5′ CCAGGA3′

[0072] Preferably, sequences that are complements of a specificallyrecited polynucleotide sequence are complementary over the entire lengthof the specific polynucleotide sequence.

[0073] Identification of genomic DNA and heterologous species DNA can beaccomplished by standard DNA/DNA hybridization techniques, underappropriately stringent conditions, using all or part of a DNA sequenceas a probe to screen an appropriate library. Alternatively, PCRtechniques using oligonucleotide primers that are designed based onknown DNA and protein sequences can be used to amplify and identifyother identical or similar DNA sequences. Synthetic DNA corresponding tothe identified sequences or variants thereof may be produced byconventional synthesis methods. All of the polynucleotides describedherein are isolated and purified, as those terms are commonly used inthe art.

[0074] The polynucleotides identified as SEQ ID NOS: 1-62 may containopen reading frames (“ORFs”), or partial open reading frames, encodingpolypeptides. Polynucleotides identified as SEQ ID NOS: 1-62 may alsocontain non-coding sequences such as promoters and terminators that maybe useful as control elements. Additionally, open reading framesencoding polypeptides may be identified in extended or full-lengthsequences corresponding to the sequences set out as SEQ ID NOS: 1-62.Open reading frames may be identified using techniques that are wellknown in the art. These techniques include, for example, analysis forthe location of known start and stop codons, most likely reading frameidentification based on codon frequencies, similarity to known bacterialexpressed genes, etc. Suitable tools and software for ORF analysisinclude GeneWise (The Sanger Center, Wellcome Trust Genome Campus,Hinxton, Cambridge CB10 1SA, United Kingdom), Diogenes (ComputationalBiology Centers, University of Minnesota, Academic Health Center, UMHGBox 43 Minneapolis Minn. 55455), and GRAIL (Informatics Group, Oak RidgeNational Laboratories, Oak Ridge, Tennessee, Tenn.). Open reading framesand portions of open reading frames may be identified in thepolynucleotides of the present invention. Once a partial open readingframe is identified, the polynucleotide may be extended in the area ofthe partial open reading frame using techniques that are well known inthe art until the polynucleotide for the full open reading frame isidentified. Thus, polynucleotides and open reading frames encodingpolypeptides may be identified using the polynucleotides of the presentinvention.

[0075] Once open reading frames are identified in the polynucleotides ofthe present invention, the open reading frames may be isolated and/orsynthesized. Expressible genetic constructs comprising the open readingframes and suitable promoters, initiators, terminators, etc., which arewell known in the art, may then be constructed. Such genetic constructsmay be introduced into a host cell to express the polypeptide encoded bythe open reading frame. Suitable host cells may include variousprokaryotic and eukaryotic cells. In vitro expression of polypeptides isalso possible, as well known in the art.

[0076] As used herein, the term “oligonucleotide” refers to a relativelyshort segment of a polynucleotide sequence, generally comprising between6 and 60 nucleotides, and comprehends both probes for use inhybridization assays and primers for use in the amplification of DNA bypolymerase chain reaction.

[0077] As used herein, the term “x-mer,” with reference to a specificvalue of“x,” refers to a polynucleotide comprising at least a specifiednumber (“x”) of contiguous residues of any of the polynucleotidesidentified as SEQ ID NOS: 1-62. The value of x may be from about 20 toabout 600, depending upon the specific sequence.

[0078] In another aspect, the present invention provides isolatedpolypeptides encoded, or partially encoded, by the abovepolynucleotides. As used herein, the term “polypeptide” encompassesamino acid chains of any length, including full-length proteins, whereinthe amino acid residues are linked by covalent peptide bonds. The term“polypeptide encoded by a polynucleotide” as used herein, includespolypeptides encoded by a polynucleotide which comprises an isolatedpolynucleotide sequence or variant provided herein. Polypeptides of thepresent invention may be naturally purified products, or may be producedpartially or wholly using recombinant techniques. Such polypeptides maybe glycosylated with bacterial, fungal, mammalian or other eukaryoticcarbohydrates or may be non-glycosylated. In specific embodiments,polypeptides of the present invention include an amino acid sequencerecited in SEQ ID NO: 63-124.

[0079] Polypeptides of the present invention may be producedrecombinantly by inserting a polynucleotide that encodes the polypeptideinto an expression vector and expressing the polypeptide in anappropriate host. Any of a variety of expression vectors known to thoseof ordinary skill in the art may be employed. Expression may be achievedin any appropriate host cell that has been transformed or transfectedwith an expression vector containing a polypeptide encoding arecombinant polypeptide. Suitable host cells include prokaryotes, yeastand higher eukaryotic cells. Preferably, the host cells employed areEscherichia coli, Lactococcus lactis, Lactobacillus, insect, yeast or amammalian cell line such as COS or CHO. The polynucleotide(s) expressedin this manner may encode naturally occurring polypeptides, portions ofnaturally occurring polypeptides, or other variants thereof.

[0080] In a related aspect, polypeptides are provided that comprise atleast a functional portion of a polypeptide having an amino acidsequence encoded by a polynucleotide of the present invention. As usedherein, a “functional portion” of a polypeptide is that portion whichcontains the active site essential for affecting the function of thepolypeptide, for example, the portion of the molecule that is capable ofbinding one or more reactants. The active site may be made up ofseparate portions present on one or more polypeptide chains and willgenerally exhibit high binding affinity.

[0081] Functional portions of a polypeptide may be identified by firstpreparing fragments of the polypeptide by either chemical or enzymaticdigestion of the polypeptide, or by mutation analysis of thepolynucleotide that encodes the polypeptide and subsequent expression ofthe resulting mutant polypeptides. The polypeptide fragments or mutantpolypeptides are then tested to determine which portions retainbiological activity, using, for example, the representative assaysprovided below.

[0082] Portions and other variants of the inventive polypeptides may begenerated by synthetic or recombinant means. Synthetic polypeptideshaving fewer than about 100 amino acids, and generally fewer than about50 amino acids, may be generated using techniques that are well known tothose of ordinary skill in the art. For example, such polypeptides maybe synthesized using any of the commercially available solid-phasetechniques, such as the Merrifield solid-phase synthesis method, whereamino acids are sequentially added to a growing amino acid chain (SeeMerrifield, J. Am. Chem. Soc. 85:2149-2154, 1963). Equipment forautomated synthesis of polypeptides is commercially available fromsuppliers such as Perkin Elmer/Applied Biosystems, Inc. (Foster City,Calif.), and may be operated according to the manufacturer'sinstructions. Variants of a native polypeptide may be prepared usingstandard mutagenesis techniques, such as oligonucleotide-directedsite-specific mutagensis (Kunkel, Proc. Natl. Acad. Sci. USA 82:488-492, 1985). Sections of DNA sequences may also be removed usingstandard techniques to permit preparation of truncated polypeptides.

[0083] In general, the polypeptides disclosed herein are prepared in anisolated, substantially pure form. Preferably, the polypeptides are atleast about 80% pure; more preferably at least about 90% pure; and mostpreferably at least about 99% pure.

[0084] As used herein, the term “variant” comprehends polynucleotide orpolypeptide sequences different from the specifically identifiedsequences, wherein one or more nucleotides or amino acid residues isdeleted, substituted, or added. Variants may be naturally occurringallelic variants, or non-naturally occurring variants. Variantpolynucleotide sequences preferably exhibit at least 40%, morepreferably at least 60%, more preferably yet at least 75%, and mostpreferably at least 90% identity to a sequence of the present invention.Variant polypeptide sequences preferably exhibit at least 50%, morepreferably at least 75%, more preferably yet at least 90%, and mostpreferably at least 95% identity to a sequence of the present invention.The percentage identity is determined by aligning the two sequences tobe compared as described below, determining the number of identicalresidues in the aligned portion, dividing that number by the totalnumber of residues in the inventive (queried) sequence, and multiplyingthe result by 100.

[0085] Polynucleotide and polypeptide sequences may be aligned, and thepercentage of identical residues in a specified region may be determinedagainst another polynucleotide or polypeptide, using computer algorithmsthat are publicly available. Two exemplary algorithms for aligning andidentifying the similarity of polynucleotide sequences are the BLASTNand FASTA algorithms. Polynucleotides may also be analyzed using theBLASTX algorithm, which compares the six-frame conceptual translationproducts of a nucleotide query sequence (both strands) against a proteinsequence database. The percentage identity of polypeptide sequences maybe examined using the BLASTP algorithm. The BLASTN, BLASTX and BLASTPprograms are available on the NCBI anonymous FTP server and from theNational Center for Biotechnology Information (NCBI), National Libraryof Medicine, Building 38A, Room 8N805, Bethesda, Md. 20894, USA. TheBLASTN algorithm Version 2.0.4 [Feb. 24, 1998], Version 2.0.6[Sept-16-1998] and Version 2.0.11 [Jan. 20, 2000], set to the parametersdescribed below, is preferred for use in the determination ofpolynucleotide variants according to the present invention. The BLASTPalgorithm, set to the parameters described below, is preferred for usein the determination of polypeptide variants according to the presentinvention. The use of the BLAST family of algorithms, including BLASTN,BLASTP and BLASTX, is described at NCBI's website and in the publicationof Altschul, et al., Nucleic Acids Res. 25:3389-3402, 1997.

[0086] The computer algorithm FASTA is available on the Internet andfrom the University of Virginia by contacting David Hudson, Vice Provostfor Research, University of Virginia, P.O. Box 9025, Charlottesville,Va. 2 2906-9025, USA. FASTA Version 2.0u4 [February 1996], set to thedefault parameters described in the documentation and distributed withthe algorithm, may be used in the determination of variants according tothe present invention. The use of the FASTA algorithm is described inPearson and Lipman, Proc. Natl. Acad. Sci. USA 85:2444-2448, 1988; andPearson, Methods in Enzymol. 183: 63-98, 1990.

[0087] The following running parameters are preferred for determinationof alignments and similarities using BLASTN that contribute to the Evalues and percentage identity for polynucleotide sequences: Unixrunning command: blastall -p blastn -d embldb -e 10 -G0 -E0 -R1 -v 30 -b30 -i queryseq -o results; the parameters are: -p Program Name [String];-d Database [String]; -e Expectation value (E) [Real]; -G Cost to open agap (zero invokes default behavior) [Integer]; -E Cost to extend a gap(zero invokes default behavior) [Integer]; -r Reward for a nucleotidematch (BLASTN only) [Integer]; -v Number of one-line descriptions (V)[Integer]; -b Number of alignments to show (B) [Integer]; -i Query File[File In]; and -o BLAST report Output File [File Out] Optional.

[0088] The following running parameters are preferred for determinationof alignments and similarities using BLASTP that contribute to the Evalues and percentage identity of polypeptide sequences: blastall -pblastp -d swissprotdb -e 10 -G 0 -E 0 -v 30 -b 30 -i queryseq -oresults; the parameters are: -p Program Name [String]; -d Database[String]; -e Expectation value (E) [Real]; -G Cost to open a gap (zeroinvokes default behavior) [Integer]; -E Cost to extend a gap (zeroinvokes default behavior) [Integer]; -v Number of one-line descriptions(v) [Integer]; -b Number of alignments to show (b) [Integer]; -I QueryFile [File In]; -o BLAST report Output File [File Out] Optional. The“hits” to one or more database sequences by a queried sequence producedby BLASTN, FASTA, BLASTP or a similar algorithm, align and identifysimilar portions of sequences. The hits are arranged in order of thedegree of similarity and the length of sequence overlap. Hits to adatabase sequence generally represent an overlap over only a fraction ofthe sequence length of the queried sequence.

[0089] The BLASTN, FASTA, and BLASTP algorithms also produce “Expect”values for alignments. The Expect value (E) indicates the number of hitsone can “expect” to see over a certain number of contiguous sequences bychance when searching a database of a certain size. The Expect value isused as a significance threshold for determining whether the hit to adatabase, such as the preferred EMBL database, indicates truesimilarity. For example, an E value of 0.1 assigned to a polynucleotidehit is interpreted as meaning that in a database of the size of the EMBLdatabase, one might expect to see 0.1 matches over the aligned portionof the sequence with a similar score simply by chance. By thiscriterion, the aligned and matched portions of the polynucleotidesequences then have a probability of 90% of being the same. Forsequences having an E value of 0.01 or less over aligned and matchedportions, the probability of finding a match by chance in the EMBLdatabase is 1% or less using the BLASTN or FASTA algorithm.

[0090] According to one embodiment, “variant” polynucleotides andpolypeptides, with reference to each of the polynucleotides andpolypeptides of the present invention, preferably comprise sequencesproducing an E value of 0.01 or less when compared to the polynucleotideor polypeptide of the present invention. That is, a variantpolynucleotide or polypeptide is any sequence that has at least a 99%probability of being the same as the polynucleotide or polypeptide ofthe present invention, measured as having an E value of 0.01 or lessusing the BLASTN, FASTA, or BLASTP algorithms set at parametersdescribed above. According to a preferred embodiment, a variantpolynucleotide is a sequence having the same number or fewer nucleicacids than a polynucleotide of the present invention that has at least a99% probability of being the same as the polynucleotide of the presentinvention, measured as having an E value of 0.01 or less using theBLASTN or FASTA algorithms set at parameters described above. Similarly,according to a preferred embodiment, a variant polypeptide is a sequencehaving the same number or fewer amino acids than a polypeptide of thepresent invention that has at least a 99% probability of being the sameas a polypeptide of the present invention, measured as having an E valueof 0.01 or less using the BLASTP algorithm set at the parametersdescribed above.

[0091] As noted above, the percentage identity is determined by aligningsequences using one of the BLASTN, FASTA, or BLASTP algorithms, set atthe running parameters described above, and identifying the number ofidentical nucleic or amino acids over the aligned portions; dividing thenumber of identical nucleic or amino acids by the total number ofnucleic or amino acids of the polynucleotide or polypeptide sequence ofthe present invention; and then multiplying by 100 to determine thepercentage identity. For example, a polynucleotide of the presentinvention having 220 nucleic acids has a hit to a polynucleotidesequence in the EMBL database having 520 nucleic acids over a stretch of23 nucleotides in the alignment produced by the BLASTN algorithm usingthe parameters described above. The 23 nucleotide hit includes 21identical nucleotides, one gap and one different nucleotide. Thepercentage identity of the polynucleotide of the present invention tothe hit in the EMBL library is thus 21/220 times 100, or 9.5%. Thepolynucleotide sequence in the EMBL database is thus not a variant of apolynucleotide of the present invention.

[0092] In addition to having a specified percentage identity to aninventive polynucleotide or polypeptide sequence, variantpolynucleotides and polypeptides preferably have additional structureand/or functional features in common with the inventive polynucleotideor polypeptide. Polypeptides having a specified degree of identity to apolypeptide of the present invention share a high degree of similarityin their primary structure and have substantially similar functionalproperties. In addition to sharing a high degree of similarity in theirprimary structure to polynucleotides of the present invention,polynucleotides having a specified degree of identity to, or capable ofhybridizing to an inventive polynucleotide preferably have at least oneof the following features: (i) they contain an open reading frame orpartial open reading frame encoding a polypeptide having substantiallythe same functional properties as the polypeptide encoded by theinventive polynucleotide; or (ii) they contain identifiable domains incommon.

[0093] Alternatively, variant polynucleotides of the present inventionhybridize to the polynucleotide sequences recited in SEQ ID NOS: 1-62,or complements, reverse sequences, or reverse complements of thosesequences, under stringent conditions. As used herein, “stringentconditions” refers to prewashing in a solution of 6×SSC, 0.2% SDS;hybridizing at 65° C., 6×SSC, 0.2% SDS overnight; followed by two washesof 30 minutes each in 1×SSC, 0.1% SDS at 65° C. and two washes of 30minutes each in 0.2×SSC, 0.1% SDS at 65° C.

[0094] The present invention also encompasses polynucleotides thatdiffer from the disclosed sequences but that, as a consequence of thediscrepancy of the genetic code, encode a polypeptide having similarenzymatic activity as a polypeptide encoded by a polynucleotide of thepresent invention. Thus, polynucleotides comprising sequences thatdiffer from the polynucleotide sequences recited in SEQ ID NOS: 1-62, orcomplements, reverse sequences, or reverse complements of thosesequences as a result of conservative substitutions are encompassedwithin the present invention. Additionally, polynucleotides comprisingsequences that differ from the inventive polynucleotide sequences orcomplements, reverse complements, or reverse sequences as a result ofdeletions and/or insertions totaling less than 10% of the total sequencelength are also contemplated by and encompassed within the presentinvention. Similarly, polypeptides comprising sequences that differ fromthe inventive polypeptide sequences as a result of amino acidsubstitutions, insertions, and/or deletions totaling less than 10% ofthe total sequence length are contemplated by and encompassed within thepresent invention, provided the variant polypeptide has similar activityto the inventive polypeptide.

[0095] The polynucleotides of the present invention may be isolated fromvarious libraries, or may be synthesized using techniques that are wellknown in the art. The polynucleotides may be synthesized, for example,using automated oligonucleotide synthesizers (e.g., Beckman Oligo 1000MDNA Synthesizer) to obtain polynucleotide segments of up to 50 or morenucleic acids. A plurality of such polynucleotide segments may then beligated using standard DNA manipulation techniques that are well knownin the art of molecular biology. One conventional and exemplarypolynucleotide synthesis technique involves synthesis of a singlestranded polynucleotide segment having, for example, 80 nucleic acids,and hybridizing that segment to a synthesized complementary 85 nucleicacid segment to produce a 5-nucleotide overhang. The next segment maythen be synthesized in a similar fashion, with a 5-nucleotide overhangon the opposite strand. The “sticky” ends ensure proper ligation whenthe two portions are hybridized. In this way, a complete polynucleotideof the present invention may be synthesized entirely in vitro.

[0096] Certain of the polynucleotides identified as SEQ ID NOS: 1-62 arereferred to as “partial” sequences, in that they do not represent thefull coding portion of a gene encoding a naturally occurringpolypeptide. The partial polynucleotide sequences disclosed herein maybe employed to obtain the corresponding full-length genes for variousspecies and organisms by, for example, screening DNA expressionlibraries using hybridization probes based on the polynucleotides of thepresent invention, or using PCR amplification with primers based uponthe polynucleotides of the present invention. In this way one can, usingmethods well known in the art, extend a polynucleotide of the presentinvention upstream and downstream of the corresponding DNA, as well asidentify the corresponding mRNA and genomic DNA, including the promoterand enhancer regions, of the complete gene. The present invention thuscomprehends isolated polynucleotides comprising a sequence identified inSEQ ID NOS: 1-62, or a variant of one of the specified sequences, thatencode a functional polypeptide, including full length genes. Suchextended polynucleotides may have a length of from about 50 to about4,000 nucleic acids or base pairs, and preferably have a length of lessthan about 4,000 nucleic acids or base pairs, more preferably yet alength of less than about 3,000 nucleic acids or base pairs, morepreferably yet a length of less than about 2,000 nucleic acids or basepairs. Under some circumstances, extended polynucleotides of the presentinvention may have a length of less than about 1,000 nucleic acids orbase pairs, preferably less than about 1,600 nucleic acids or basepairs, more preferably less than about 1,400 nucleic acids or basepairs, more preferably yet less than about 1,200 nucleic acids or basepairs, and most preferably less than about 1,000 nucleic acids or basepairs.

[0097] Polynucleotides of the present invention comprehendpolynucleotides comprising at least a specified number of contiguousresidues (x-mers) of any of the polynucleotides identified as SEQ IDNOS: 1-62 or their variants. According to preferred embodiments, thevalue of x is preferably at least 20, more preferably at least 40, morepreferably yet at least 60, and most preferably at least 80. Thus,polynucleotides of the present invention include polynucleotidescomprising a 20-mer, a 40-mer, a 60-mer, an 80-mer, a 100-mer, a120-mer, a 150-mer, a 180-mer, a 220-mer a 250-mer, or a 300-mer,400-mer, 500-mer or 600-mer of a polynucleotide identified as SEQ IDNOS: 1-62 or a variant of one of the polynucleotides identified as SEQID NOS: 1-62.

[0098] Oligonucleotide probes and primers complementary to and/orcorresponding to SEQ ID NOS: 1-62, and variants of those sequences, arealso comprehended by the present invention. Such oligonucleotide probesand primers are substantially complementary to the polynucleotide ofinterest. An oligonucleotide probe or primer is described as“corresponding to” a polynucleotide of the present invention, includingone of the sequences set out as SEQ ID NOS: 1-62 or a variant, if theoligonucleotide probe or primer, or its complement, is contained withinone of the sequences set out as SEQ ID NOS: 1-62 or a variant of one ofthe specified sequences.

[0099] Two single stranded sequences are said to be substantiallycomplementary when the nucleotides of one strand, optimally aligned andcompared, with the appropriate nucleotide insertions and/or deletions,pair with at least 80%, preferably at least 90% to 95%, and morepreferably at least 98% to 100%, of the nucleotides of the other strand.Alternatively, substantial complementarity exists when a first DNAstrand will selectively hybridize to a second DNA strand under stringenthybridization conditions. Stringent hybridization conditions fordetermining complementarity include salt conditions of less than about 1M, more usually less than about 500 mM and preferably less than about200 mM. Hybridization temperatures can be as low as 5° C., but aregenerally greater than about 22° C., more preferably greater than about30° C. and most preferably greater than about 37° C. Longer DNAfragments may require higher hybridization temperatures for specifichybridization. Since the stringency of hybridization may be affected byother factors such as probe composition, presence of organic solventsand extent of base mismatching, the combination of parameters is moreimportant than the absolute measure of any one alone. DNA-DNAhybridization studies may performed using either genomic DNA or DNAderived by preparing cDNA from the RNA present in a sample to be tested.

[0100] In addition to DNA-DNA hybridization, DNA-RNA or RNA-RNAhybridization assays are also possible. In the first case, the mRNA fromexpressed genes would then be detected instead of genomic DNA or cDNAderived from mRNA of the sample. In the second case, RNA probes could beused. In addition, artificial analogs of DNA hybridizing specifically totarget sequences could also be used.

[0101] In specific embodiments, the oligonucleotide probes and/orprimers comprise at least about 6 contiguous residues, more preferablyat least about 10 contiguous residues, and most preferably at leastabout 20 contiguous residues complementary to a polynucleotide sequenceof the present invention. Probes and primers of the present inventionmay be from about 8 to 100 base pairs in length or, preferably fromabout 10 to 50 base pairs in length or, more preferably from about 15 to40 base pairs in length. The primers and probes may be readily selectedusing procedures well known in the art, taking into account DNA-DNAhybridization stringencies, annealing and melting temperatures,potential for formation of loops and other factors, which are well knownin the art. Tools and software suitable for designing probes, andespecially suitable for designing PCR primers, are available on theInternet. In addition, a software program suitable for designing probes,and especially for designing PCR primers, is available from PremierBiosoft International, 3786 Corina Way, Palo Alto, Calif. 94303-4504.Preferred techniques for designing PCR primers are also disclosed inDieffenbach and Dyksler, PCR primer: a laboratory manual, CSHL Press:Cold Spring Harbor, N.Y., 1995.

[0102] A plurality of oligonucleotide probes or primers corresponding toa polynucleotide of the present invention may be provided in a kit form.Such kits generally comprise multiple DNA or oligonucleotide probes,each probe being specific for a polynucleotide sequence. Kits of thepresent invention may comprise one or more probes or primerscorresponding to a polynucleotide of the present invention, including apolynucleotide sequence identified in SEQ ID NOS: 1-62.

[0103] In one embodiment useful for high-throughput assays, theoligonucleotide probe kits of the present invention comprise multipleprobes in an array format, wherein each probe is immobilized in apredefined, spatially addressable location on the surface of a solidsubstrate. Array formats which may be usefully employed in the presentinvention are disclosed, for example, in U.S. Pat. Nos. 5,412,087,5,545,531, and PCT Publication No. WO 95/00530, the disclosures of whichare hereby incorporated by reference.

[0104] Oligonucleotide probes for use in the present invention may beconstructed synthetically prior to immobilization on an array, usingtechniques well known in the art (See, for example, Gait, ed.,Oligonucleotide synthesis a practical approach, IRL Press: Oxford,England, 1984). Automated equipment for the synthesis ofoligonucleotides is available commercially from such companies as PerkinElmer/Applied Biosystems Division (Foster City, Calif.) and may beoperated according to the manufacturer's instructions. Alternatively,the probes may be constructed directly on the surface of the array usingtechniques taught, for example, in PCT Publication No. WO 95/00530.

[0105] The solid substrate and the surface thereof preferably form arigid support and are generally formed from the same material. Examplesof materials from which the solid substrate may be constructed includepolymers, plastics, resins, membranes, polysaccharides, silica orsilica-based materials, carbon, metals and inorganic glasses.Synthetically prepared probes may be immobilized on the surface of thesolid substrate using techniques well known in the art, such as thosedisclosed in U.S. Pat. No. 5,412,087.

[0106] In one such technique, compounds having protected functionalgroups, such as thiols protected with photochemically removableprotecting groups, are attached to the surface of the substrate.Selected regions of the surface are then irradiated with a light source,preferably a laser, to provide reactive thiol groups. This irradiationstep is generally performed using a mask having apertures at predefinedlocations using photolithographic techniques well known in the art ofsemiconductors. The reactive thiol groups are then incubated with theoligonucleotide probe to be immobilized. The precise conditions forincubation, such as temperature, time and pH, depend on the specificprobe and can be easily determined by one of skill in the art. Thesurface of the substrate is washed free of unbound probe and theirradiation step is repeated using a second mask having a differentpattern of apertures. The surface is subsequently incubated with asecond, different, probe. Each oligonucleotide probe is typicallyimmobilized in a discrete area of less than about 1 mm². Preferably eachdiscrete area is less than about 10,000 mm, more preferably less thanabout 100 mm². In this manner, a multitude of oligonucleotide probes maybe immobilized at predefined locations on the array.

[0107] The resulting array may be employed to screen for differences inorganisms or samples or products containing genetic material as follows.Genomic or cDNA libraries are prepared using techniques well known inthe art. The resulting target DNA is then labeled with a suitablemarker, such as a radiolabel, chromophore, fluorophore orchemiluminescent agent, using protocols well known for those skilled inthe art. A solution of the labeled target DNA is contacted with thesurface of the array and incubated for a suitable period of time.

[0108] The surface of the array is then washed free of unbound targetDNA and the probes to which the target DNA hybridized are determined byidentifying those regions of the array to which the markers areattached. When the marker is a radiolabel, such as ³²P, autoradiographyis employed as the detection method. In one embodiment, the marker is afluorophore, such as fluorescein, and the location of bound target DNAis determined by means of fluorescence spectroscopy. Automated equipmentfor use in fluorescence scanning of oligonucleotide probe arrays isavailable from Affymetrix, Inc. (Santa Clara, Calif.) and may beoperated according to the manufacturer's instructions. Such equipmentmay be employed to determine the intensity of fluorescence at eachpredefined location on the array, thereby providing a measure of theamount of target DNA bound at each location. Such an assay would be ableto indicate not only the absence and presence of the marker probe in thetarget, but also the quantitative amount as well.

[0109] The significance of such high-throughput screening system isapparent for applications such as microbial selection and qualitycontrol operations in which there is a need to identify large numbers ofsamples or products for unwanted materials, to identify microbes orsamples or products containing microbial material for quarantinepurposes, etc., or to ascertain the true origin of samples or productscontaining microbes. Screening for the presence or absence ofpolynucleotides of the present invention used as identifiers for taggingmicrobes and microbial products can be valuable for later detecting thegenetic composition of food, fermentation and industrial microbes ormicrobes in human or animal digestive system after consumption ofprobiotics, etc.

[0110] In this manner, oligonucleotide probe kits of the presentinvention may be employed to examine the presence/absence (or relativeamounts in case of mixtures) of polynucleotides in different samples orproducts containing different materials rapidly and in a cost-effectivemanner. Examples of microbial species which may be examined using thepresent invention, include lactic acid bacteria, such as Lactobacillusrhamnosus, and other microbial species.

[0111] Another aspect of the present invention involves collections of aplurality of polynucleotides of the present invention. A collection of aplurality of the polynucleotides of the present invention, particularlythe polynucleotides identified as SEQ ID NOS: 1-62, may be recordedand/or stored on a storage medium and subsequently accessed for purposesof analysis, comparison, etc. Suitable storage media include magneticmedia such as magnetic diskettes, magnetic tapes, CD-ROM storage media,optical storage media, and the like. Suitable storage media and methodsfor recording and storing information, as well as accessing informationsuch as polynucleotide sequences recorded on such media, are well knownin the art. The polynucleotide information stored on the storage mediumis preferably computer-readable and may be used for analysis andcomparison of the polynucleotide information.

[0112] Another aspect of the present invention thus involves storagemedium on which are recorded a collection of the polynucleotides of thepresent invention, particularly a collection of the polynucleotidesidentified as SEQ ID NOS: 1-62. According to one embodiment, the storagemedium includes a collection of at least 20, preferably at least 50,more preferably at least 100, and most preferably at least 200 of thepolynucleotides of the present invention, preferably the polynucleotidesidentified as SEQ ID NOS: 1-62, including variants of thosepolynucleotides.

[0113] Another aspect of the present invention involves a combination ofpolynucleotides, the combination containing at least 5, preferably atleast 10, more preferably at least 20, and most preferably at least 50different polynucleotides of the present invention, includingpolynucleotides selected from SEQ ID NOS: 1-62, and variants of thesepolynucleotides.

[0114] In another aspect, the present invention provides geneticconstructs comprising, in the 5′-3′ direction, a gene promoter sequence;and an open reading frame coding for at least a functional portion of apolypeptide encoded by a polynucleotide of the present invention. Incertain embodiments, the genetic constructs of the present inventionalso comprise a gene termination sequence. The open reading frame may beoriented in either a sense or antisense direction. Genetic constructscomprising a non-coding region of a gene coding for a polypeptideencoded by the above polynucleotides or a nucleotide sequencecomplementary to a non-coding region, together with a gene promotersequence, are also provided. A terminator sequence may form part of thisconstruct. Preferably, the gene promoter and termination sequences arefunctional in a host organism. More preferably, the gene promoter andtermination sequences are common to those of the polynucleotide beingintroduced. The genetic construct may further include a marker for theidentification of transformed cells.

[0115] Techniques for operatively linking the components of the geneticconstructs are well known in the art and include the use of syntheticlinkers containing one or more restriction endonuclease sites asdescribed, for example, by Sambrook et al., in Molecular cloning: alaboratory manual, Cold Spring Harbor Laboratories Press: Cold SpringHarbor, N.Y., 1989. The genetic constructs of the present invention maybe linked to a vector having at least one replication system, forexample, E. coli, whereby after each manipulation, the resultingconstruct can be cloned and sequenced and the correctness of themanipulation determined.

[0116] Transgenic microbial cells comprising the genetic constructs ofthe present invention are also provided by the present invention,together with microbes comprising such transgenic cells, products andprogeny of such microbes, and materials including such microbes.Techniques for stably incorporating genetic constructs into the genomeof target microbes, such as Lactobacillus species, Lactococcus lactis orE. coli, are well known in the art of bacterial transformation and areexemplified by the transformation of E. coli for sequencing in Example1.

[0117] Transgenic, non-microbial, cells comprising the geneticconstructs of the present invention are also provided, together withorganisms comprising such transgenic cells, and products and progeny ofsuch organisms. Genetic constructs of the present invention may bestably incorporated into the genomes of non-microbial target organisms,such as fungi, using techniques well known in the art.

[0118] In preferred embodiments, the genetic constructs of the presentinvention are employed to transform microbes used in the production offood products, ingredients, processing aids, additives or supplementsand for the production of microbial products for pharmaceutical uses,particularly for modulating immune system function and immunologicaleffects; and in the production of chemoprotectants providing beneficialeffects, probiotics and health supplements. The inventive geneticconstructs may also be employed to transform bacteria that are used toproduce enzymes or substances such as polysaccharides, flavor compounds,and bioactive substances, and to enhance resistance to industrialprocesses such as drying and to adverse stimuli in the human digestivesystem. The genes involved in antibiotic production, and phage uptakeand resistance in Lactobacillus rhamnosus are considered to beespecially useful. The target microbe to be used for transformation withone or more polynucleotides or genetic constructs of the presentinvention is preferably selected from the group consisting of bacterialgenera Lactococcus, Lactobacillus, Streptococcus, Oenococcus,Lactosphaera, Trichococcus, Pediococcus and others potentially useful invarious fermentation industries selected, most preferably, from thegroup consisting of Lactobacillus species in the following list:Lactobacillus acetotolerans, Lactobacillus acidophilus, Lactobacillusagilis, Lactobacillus alimentarius, Lactobacillus amylolyticus,Lactobacillus amylophilus, Lactobacillus amylovorus, Lactobacillusanimalis, Lactobacillus arizonae, Lactobacillus aviarius, Lactobacillusbavaricus, Lactobacillus bifermentans, Lactobacillus brevis,Lactobacillus buchneri, Lactobacillus bulgaricus, Lactobacillus casei,Lactobacillus collinoides, Lactobacillus coryniformis, Lactobacilluscrispatus, Lactobacillus curvatus, Lactobacillus delbrueckii,Lactobacillus delbrueckii subsp. bulgaricus, Lactobacillus delbrueckiisubsp. lactis, Lactobacillus farciminis, Lactobacillus fermentum,Lactobacillus fructivorans, Lactobacillus gallinarum, Lactobacillusgasseri, Lactobacillus graminis, Lactobacillus hamsteri, Lactobacillushelveticus, Lactobacillus helveticus subsp. jugurti, Lactobacillushetero, Lactobacillus hilgardii, Lactobacillus homohiochii,Lactobacillus japonicus, Lactobacillus johnsonii, Lactobacillus kefiri,Lactobacillus lactis, Lactobacillus leichmannii, Lactobacillus lindneri,Lactobacillus mali, Lactobacillus maltaromicus, Lactobacillusmanihotivorans, Lactobacillus mucosae, Lactobacillus murinus,Lactobacillus oris, Lactobacillus panis, Lactobacillus paracasei,Lactobacillus paracasei subsp. pseucloplantarum, Lactobacillusparaplantarum, Lactobacillus pentosus, Lactobacillus plantarum,Lactobacillus pontis, Lactobacillus reuteri, Lactobacillus rhamnosus,Lactobacillus ruminis, Lactobacillus sake, Lactobacillus salivarius,Lactobacillus salivarius subsp. salicinius, Lactobacillus salivariussubsp. salivarius, Lactobacillus sanfranciscensis, Lactobacillussharpeae, Lactobacillus thermophilus, Lactobacillus vaginalis,Lactobacillus vermiforme, Lactobacillus zeae.

[0119] In yet a further aspect, the present invention provides methodsfor modifying the concentration, composition and/or activity of apolypeptide in a host organism, such as a microbe, comprising stablyincorporating a genetic construct of the present invention into thegenome of the host organism by transforming the host organism with sucha genetic construct. The genetic constructs of the present invention maybe used to transform a variety of organisms. Organisms which may betransformed with the inventive constructs include plants, such asmonocotyledonous angiosperms (e.g., grasses, corn, grains, oat, wheatand barley); dicotyledonous angiosperms (e.g., Arabidopsis, tobacco,legumes, alfalfa, oaks, eucalyptus, maple); gymnosperms, (e.g., Scotspine (Aronen, Finnish Forest Res. Papers, Vol. 595, 1996); white spruce(Ellis et al., Biotechnology 11:84-89, 1993); and larch (Huang, et al.,In Vitro Cell 27:201-207, 1991); and any kind of plant amenable togenetic engineering.

[0120] Thus, in yet another aspect, transgenic plant cells comprisingthe genetic constructs of the present invention are provided, togetherwith plants comprising such transgenic cells, and fruits, seeds,products and progeny of such plants. Techniques for stably incorporatinggenetic constructs into the genome of target organisms, such as plants,are well known in the art and include Agrobacterium tumefaciens mediatedintroduction, electroporation, protoplast fusion, injection intoreproductive organs, injection into immature embryos, high velocityprojectile introduction and the like. The choice of technique willdepend upon the target plant to be transformed. For example,dicotyledonous plants and certain monocots and gymnosperms may betransformed by Agrobacterium Ti plasmid technology, as described, forexample by Bevan, Nucleic Acids Res. 12:8711-8721, 1984. Targets for theintroduction of the genetic constructs include tissues, such as leaftissue, disseminated cells, protoplasts, seeds, embryos, meristematicregions; cotyledons, hypocotyls, and the like.

[0121] Once the cells are transformed, cells having the geneticconstruct incorporated in their genome are selected. Transgenic cellsmay then be cultured in an appropriate medium, using techniques wellknown in the art. In the case of protoplasts, the cell wall is allowedto reform under appropriate osmotic conditions. In the case of seeds orembryos, an appropriate germination or callus initiation medium isemployed.

[0122] The polynucleotides of the present invention may be furtheremployed as non-disruptive tags for marking organisms, particularlymicrobes. Other organisms may, however, be tagged with thepolynucleotides of the present invention, including commerciallyvaluable plants, animals, fish, fungi and yeasts. Genetic constructscomprising polynucleotides of the present invention may be stablyintroduced into an organism as heterologous, non-functional,non-disruptive tags. It is then possible to identify the origin orsource of the organism at a later date by determining the presence orabsence of the tag(s) in a sample of material. Detection of the tag(s)may be accomplished using a variety of conventional techniques, and willgenerally involve the use of nucleic acid probes. Sensitivity inassaying the presence of probe can be usefully increased by usingbranched oligonucleotides, as described by Horn el al., Nucleic AcidsRes. 25(23):4842-4849, 1997, enabling detection of as few as 50 DNAmolecules in the sample.

[0123] Polynucleotides of the present invention may also be used tospecifically suppress gene expression by methods that operatepost-transcriptionally to block the synthesis of products of targetedgenes, such as RNA interference (RNAi), and quelling. For a review oftechniques of gene suppression see Science, 288:1370-1372, 2000.Exemplary gene silencing methods are also provided in WO 99/49029 and WO99/53050. Posttranscriptional gene silencing is brought about by asequence-specific RNA degradation process which results in the rapiddegradation of transcripts of sequence-related genes. Studies haveprovided evidence that double-stranded RNA may act as a mediator ofsequence-specific gene silencing (see, e.g., review by Montgomery andFire, Trends in Genetics, 14: 255-258, 1998). Gene constructs thatproduce transcripts with self-complementary regions are particularlyefficient at gene silencing. A unique feature of thisposttranscriptional gene silencing pathway is that silencing is notlimited to the cells where it is initiated. The gene-silencing effectsmay be disseminated to other parts of an organism and even transmittedthrough the germ line to several generations.

[0124] The polynucleotides of the present invention may be employed togenerate gene silencing constructs and or gene-specificself-complementary RNA sequences that can be delivered by conventionalart-known methods to cells and tissues. Within genetic constructs, senseand antisense sequences can be placed in regions flanking an intronsequence in proper splicing orientation with donor and acceptor splicingsites, such that intron sequences are removed during processing of thetranscript and sense and antisense sequences, as well as splice junctionsequences, bind together to form double-stranded RNA. Alternatively,spacer sequences of various lengths may be employed to separateself-complementary regions of sequence in the construct. Duringprocessing of the gene construct transcript, intron sequences arespliced-out, allowing sense and anti-sense sequences, as well as splicejunction sequences, to bind forming double-stranded RNA. Selectribonucleases bind to and cleave the double-stranded RNA, therebyinitiating the cascade of events leading to degradation of specific mRNAgene sequences, and silencing specific genes. Alternatively, rather thanusing a gene construct to express the self-complementary RNA sequences,the gene-specific double-stranded RNA segments are delivered to one ormore targeted areas to be internalized into the cell cytoplasm to exerta gene silencing effect. Gene silencing RNA sequences comprising thepolynucleotides of the present invention are useful for creatinggenetically modified organisms with desired phenotypes as well as forcharacterizing genes (e.g., in high-throughput screening of sequences),and studying their functions in intact organisms.

[0125] In another aspect, the present invention provides methods forusing one or more of the inventive polypeptides or polynucleotides totreat disorders in a mammal, such as a human.

[0126] In this aspect, the polypeptide or polynucleotide is generallypresent within a composition, such as a pharmaceutical or immunogeniccomposition. Pharmaceutical compositions may comprise one or morepolypeptides, each of which may contain one or more of the abovesequences (or variants thereof), and a physiologically acceptablecarrier. Immunogenic compositions may comprise one or more of the abovepolypeptides and an immunostimulant, such as an adjuvant or a liposome,into which the polypeptide is incorporated.

[0127] Alternatively, a composition of the present invention may containDNA encoding one or more polypeptides described herein, such that thepolypeptide is generated in situ. In such compositions, the DNA may bepresent within any of a variety of delivery systems known to those ofordinary skill in the art, including nucleic acid expression systems,and bacterial and viral expression systems. Appropriate nucleic acidexpression systems contain the necessary DNA sequences for expression inthe patient (such as a suitable promoter and terminator signal).Bacterial delivery systems involve the administration of a bacterium(such as Bacillus Calmette-Guerin) that expresses an immunogenic portionof the polypeptide on its cell surface. In a preferred embodiment, theDNA may be introduced using a viral expression system (e.g., vaccinia orother poxvirus, retrovirus, or adenovirus), which may involve the use ofa non-pathogenic, or defective, replication competent virus. Techniquesfor incorporating DNA into such expression systems are well known in theart. The DNA may also be “naked,” as described, for example, in Ulmer etal., Science 259:1745-1749, 1993 and reviewed by Cohen, Science259:1691-1692, 1993. The uptake of naked DNA may be increased by coatingthe DNA onto biodegradable beads, which are efficiently transported intothe cells.

[0128] While any suitable carrier known to those of ordinary skill inthe art may be employed in the pharmaceutical compositions of thisinvention, the type of carrier will vary depending on the mode ofadministration. For parenteral administration, such as subcutaneousinjection, the carrier preferably comprises water, saline, alcohol, alipid, a wax or a buffer. For oral administration, any of the abovecarriers or a solid carrier, such as mannitol, lactose, starch,magnesium stearate, sodium saccharine, talcum, cellulose, glucose,sucrose, and magnesium carbonate, may be employed. Biodegradablemicrospheres (e.g., polylactic galactide) may also be employed ascarriers for the pharmaceutical compositions of this invention. Suitablebiodegradable microspheres are disclosed, for example, in U.S. Pat. Nos.4,897,268 and 5,075,109.

[0129] Any of a variety of adjuvants may be employed in the immunogeniccompositions of the present invention to non-specifically enhance animmune response. Most adjuvants contain a substance designed to protectthe antigen from rapid catabolism, such as aluminum hydroxide or mineraloil, and a non-specific stimulator of immune responses, such as lipid A,Bordetella pertussis or M. tuberculosis. Suitable adjuvants arecommercially available as, for example, Freund's Incomplete Adjuvant andFreund's Complete Adjuvant (Difco Laboratories, Detroit, Mich.), andMerck Adjuvant 65 (Merck and Company, Inc., Rahway, N.J.). Othersuitable adjuvants include alum, biodegradable microspheres,monophosphoryl lipid A and Quil A.

[0130] Routes and frequency of administration, as well as dosage, varyfrom individual to individual. In general, the inventive compositionsmay be administered by injection (e.g., intradermal, intramuscular,intravenous or subcutaneous), intranasally (e.g., by aspiration) ororally. In general, the amount of polypeptide present in a dose (orproduced in situ by the DNA in a dose) ranges from about 1 pg to about100 mg per kg of host, typically from about 10 pg to about 1 mg per kgof host, and preferably from about 100 pg to about 1 μg per kg of host.Suitable dose sizes will vary with the size of the patient, but willtypically range from about 0.1 ml to about 2 ml.

[0131] The following examples are offered by way of illustration and notby way of limitation.

EXAMPLE 1 Isolation and Characterization of DNA Sequences fromLactobacillus rhamnosus Strain HN001

[0132]Lactobacillus rhamnosus strain HN001 DNA libraries wereconstructed and screened as follows.

[0133] DNA was prepared in large scale by cultivating the bacteria in2×100 ml cultures with 100 ml MRS broth (Difco Laboratories, DetroitMich.) and 1 ml Lactobacillus glycerol stock as inoculum, placed into500 ml culture flasks and incubated at 37° C. for approx. 16 hours withshaking (220 rpm).

[0134] The cultures were centrifuged at 6200 rpm for 10 min to pelletthe cells. The supernatant was removed and the cell pellet resuspendedin 40 ml fresh MRS broth and transferred to clean 500 ml culture flasks.Fresh MRS broth (60 ml) was added to bring the volume back to 100 ml andflasks were incubated for a further 2 hrs at 37° C. with shaking (220rpm). The cells were pelleted by centrifugation (6200 rpm for 10 min)and supernatant removed. Cell pellets were washed twice in 20 ml bufferA (50 mM NaCl, 30 mM Tris pH 8.0, 0.5 mM EDTA).

[0135] Cells were resuspended in 2.5 ml buffer B (25% sucrose (w/v), 50mM Tris pH 8.0, 1 mM EDTA, 20 mg/ml lysozyme, 20 μg/ml mutanolysin) andincubated at 37° C. for 45 min. Equal volumes of EDTA (0.25 M) was addedto each tube and allowed to incubate at room temperature for 5 min. 20%SDS (1 ml) solution was added, mixed and incubated at 65° C. for 90 min.50 μl Proteinase K (Gibco BRL, Gaithersburg, Md.) from a stock solutionof 20 mg/ml was added and tubes incubated at 65° C. for 15 min.

[0136] DNA was extracted with equal volumes ofphenol:chloroform:isoamylalcohol (25:24:1). Tubes were centrifuged at6200 rpm for 40 min. The aqueous phase was removed to clean sterile OakRidge centrifuge tubes (30 ml). Crude DNA was precipitated with an equalvolume of cold isopropanol and incubated at −20° C. overnight.

[0137] After resuspension in 500 μl TE buffer, DNase-free RNase wasadded to a final concentraion of 100 μg/ml and incubated at 37° C. for30 min. The incubation was extended for a further 30 min after adding100 μl Proteinase K from a stock solution of 20 mg/ml. DNA wasprecipitated with ethanol after a phenol:chloroform:isoamylalcohol(25:24:1) and a chloroform:isoamylalcohol (24:1) extraction anddissolved in 250 μl TE buffer.

[0138] DNA was digested with Sau3AI at a concentration of 0.004 U/μg ina total volume of 1480 μl, with 996 μl DNA, 138.75 μl 10×REACT 4 bufferand 252.75 μl H₂O. Following incubation for 1 hour at 37° C., DNA wasdivided into two tubes. 31 μl 0.5 M EDTA was added to stop the digestionand 17 μl samples were taken for agarose gel analysis. Samples were putinto 15 ml Falcon tubes and diluted to 3 ml for loading onto sucrosegradient tubes.

[0139] Sucrose gradient size fractionation was conducted as follows. 100ml of 50% sucrose (w/v) was made in TEN buffer (1M NaCl, 20 mM Tris pH8.0, 5 mM EDTA) and sterile filtered. Dilutions of 5, 10, 15, 20, 25,30, 62 and 40% sucrose were prepared and overlaid carefully in BeckmanPolyallomer tubes, and kept overnight at 4° C. TEN buffer (4 ml) wasloaded onto the gradient, with 3 ml of DNA solution on top. Thegradients were centrifuged at 26K for 18 hours at 4° C. in a CentriconT-2060 centrifuge using a Kontron TST 28-38 rotor. After decelerationwithout braking (approx. 1 hour), the gradients were removed andfractions collected using an auto Densi-Flow (Haake-BuchlerInstruments). Agarose gel was used to analyse the fractions. The besttwo pairs of fractions were pooled and diluted to contain less than 10%sucrose. TEN buffer (4 ml) was added and DNA precipitated with 2 volumesof 100% ice cold ethanol and an overnight incubation at −20° C.

[0140] DNA pellets were resuspended in 300 μl TE buffer andre-precipitated for approx. 6 hours at −20° C. after adding 1/10 volume3 M NaOAC pH 5.2 and 2 volumes of ethanol. DNA was pelleted at top speedin a microcentrifuge for 15 min, washed with 70% ethanol and pelletedagain, dried and resuspended in 10 μl TE buffer.

[0141] DNA was ligated into dephosphorylated BamHI-digested pBluescriptSK II⁺ and dephosphorylated BamHI-digested lambda ZAP Express usingstandard protocols. Packaging of the DNA was done using Gigapack IIIGold packaging extract (Stratagene, La Jolla, Calif.) following themanufacturer's protocols. Packaged libraries were stored at 4° C.

[0142] Mass excision from the primary packaged phage library was doneusing XL1-Blue MRF′ cells and ExAssist Helper Phage (Stratagene). Theexcised phagemids were diluted with NZY broth (Gibco BRL, Gaithersburg,Md.) and plated out onto LB-kanamycin agar plates containing5-bromo-4-chloro-3-indolyl-β-D-galactoside (X-gal) andisopropylthio-beta-galactoside (IPTG). After incubation, single colonieswere picked for PCR size determination before the most suitablelibraries were selected for sequencing.

[0143] Of the colonies picked for DNA minipreps and subsequentsequencing, the large majority contained an insert suitable forsequencing. Positive colonies were cultured in LB broth with kanamycinor ampicillin depending on the vector used, and DNA was purified bymeans of rapid alkaline lysis minipreps (solutions: Qiagen, Venlo, TheNetherlands; clearing plates, Millipore, Bedford, Mass.). Agarose gelsat 1% were used to screen sequencing templates for chromosomalcontamination and concentration. Dye terminator sequencing reactionswere prepared using a Biomek 2000 robot (Beckman Coulter, Inc.,Fullerton, Calif.) and Hydra 96 (Robbins Scientific, Sunnyvale, Calif.)for liquid handling. DNA amplification was done in a 9700 PCR machine(Perkin Elmer/Applied Biosystems, Foster City, Calif.) according to themanufacturer's protocol.

[0144] The sequence of the genomic DNA fragments were determined using aPerkin Elmer/Applied Biosystems Division Prism 377 sequencer.

[0145] To extend the sequences of the inserts from these clones, primerswere designed from the determined nucleotide sequences so that theprimer sequences are located approximately 100 bp downstream of the 5′end and 100 bp upstream of the 3′ end of the determined nucleotidesequence. Selection of primers were done with the Gap4 Genome AssemblyProgram (Bonfield et al., Nucleic Acids Res. 24:4992-4999, 1995 usingthe following parameters: No. of bases ahead: 40; No. of bases back: 40;Minimum melting temperature: 55° C.; maximum melting temperature: 60°C.; minimum length: 17 bp; maximum length: 20 bp; minimum GC-content:40%; maximum GC-content: 60%. Sequencing of clones was done as describedabove. The determined nucleotide sequences are identified as SEQ ID NOS:1-62 disclosed herein.

[0146] This example not only shows how the sequences were obtained, butalso that a bacterium (E. coli) can be stably transformed with anydesired DNA fragment of the present invention for permanent marking forstable inheritance.

[0147] The determined DNA sequences were compared to and aligned withknown sequences in the public databases. Specifically, thepolynucleotides identified in SEQ ID NO: 1-62 were compared topolynucleotides in the EMBL database as of the end of July 2000, usingBLASTN algorithm Version 2.0.11 [Jan-20-2000], set to the followingrunning parameters: Unix running command: blastall -p blastn -d embldb-e 10 -G 0 -E 0 -r 1 -v 30 -b 30 -i querseuq -o results. Multiplealignments of redundant sequences were used to build up reliableconsensus sequences. Based on similarity to known sequences, theisolated polynucleotides of the present invention identified as SEQ IDNOS: 1 -62 were putatively identified as encoding polypeptides havingsimilarity to the polypeptides shown above in Table 1. The amino acidsequences encoded by the DNA sequences of SEQ ID NO: 1-62 are providedin SEQ ID NO: 63-124, respectively.

[0148] Several of the sequences provided in SEQ ID NO: 1-62 were foundto be full-length and to contain open reading frames (ORFs).Specifically, SEQ ID NOS: 1, 2, 4-12, 14, 20, 21, 24, 26, 34, 36, 42,44, 45, 54, 55, 59 and 61 were found to be full-length. The location ofORFs (by nucleotide position) contained within SEQ ID NOS: 1-62, and thecorresponding amino acid sequences are provided in Table 2 below. TABLE2 Polynucleotide Polypeptide SEQ ID NO: Open reading frame SEQ ID NO: 1 1-672 63 2  1-1,419 64 3  1-1,104 65 4  1-1,107 66 5  1-1,170 67 6 1-891 68 7  1-1,170 69 8  1-1,158 70 9  1-786 71 10  1-927 72 11  1-81073 12  1-1,422 74 13  1-768 75 14  1-1,923 76 15  1-1,443 77 16  1-99378 17  1-1,032 79 18  1-1,674 80 19  1-876 81 20  1-732 82 21  1-1,29983 22  1-1,344 84 23  1-474 85 24  1-1,002 86 25  1-1,239 87 26  1-1,88188 27  1-606 89 28  1-1,023 90 29  1-1,227 91 30  1-1,158 92 31  1-1,07193 32  1-1,308 94 33  1-645 95 34  1-1,920 96 35  1-762 97 36  1-936 9837  1-840 99 38  1-1,341 100 39  1-726 101 40  1-972 102 41  1-888 10342  1-1,422 104 43  1-774 105 44  1-1,254 106 45  1-489 107 46  1-285108 47  1-969 109 48 417-1,336 110 49  1-760 111 50 193-846 112 51463-1,310 113 52 628-1,662 114 53  1-887 115 54 251-946 116 55  66-743117 56  1-780 118 57 256-1,569 119 58 274-1,112 120 59  8-954 121 60 17-948 122 61 206-1,006 123 62  1-1,563 124

[0149] SEQ ID NOS: 1-124 are set out in the attached Sequence Listing.The codes for nucleotide sequences used in the attached SequenceListing, including the symbol “n,” conform to WIPO Standard ST.25(1998), Appendix 2, Table 1.

[0150] While in the foregoing specification this invention has beendescribed in relation to certain preferred embodiments, and many detailshave been set forth for purposes of illustration, it will be apparent tothose skilled in the art that the invention is susceptible to additionalembodiments and that certain of the details described herein may bevaried considerably without departing from the basic principles of theinvention.

1 124 1 711 DNA Lactobacillus rhamnosus 1 atgtggaaag ttattaccgatgcagttccg caaatgatag cagccggcat taaatacacc 60 attccaattg cactcgtgtcatttgcgatc ggcctgatca tcgcacttgt aaccgcattg 120 acgcgcatat cggttcgtaaaggtattttg atccgaatcg caaaaggaat cgccgttttt 180 tacgtttggc tctttcgctcaacgcctttg ctggtgcagt tattcatcgt tttcttcggc 240 ttacccagcc tcatcatcccgggtattttc ccgcatggca tcaagttaga tcccgcggcc 300 gcgggaatta taacattctcacttaacacg ggggcgtatt gtgccgaaac gacccgcgcc 360 tcgctgttgt cgattgattccgggcaatgg gaagcggctt atgcaattgg attgccgcgg 420 cgactggtgc tgcgcgaaatcattattcct caagcactac gaacggccat cccgccactg 480 tcaaatagtt tcatcagcctgatcaaagac acatcccttg ctgcctcgat caccattgtc 540 gaaatgtttc aagtcagccaacaaatcgcg gcggaaaatt accaaccatt actgatgtac 600 tcaatcgttg cgcttctgtatgccattgtc tgcactttct tagcttgggg tcagcggtat 660 ctcgaaaaat tcacatcacgctacaatgcc aatgcacaaa ccacgcaatt a 711 2 1425 DNA Lactobacillusrhamnosus 2 ctgatttgca aaggtcgaag cttgaagcca ttcggacatt ttattgatgccatcacggta 60 aatcgagaac acgtcttgac aaccgctgcc gaggccttga ttgcaagtgcgggcgatgcc 120 ttaaatgcca gtcacgcgac cttcaatgta ttaaacaact ctgatctgcaattcgggttt 180 gttgaaaatg aagatggcga aaccgtccag cttagtaacg gtttgtacggtcagttgatt 240 cgctcgacta accgtaaatt acgcaaggag gctttcgagg cccttctgcgtgcttacgaa 300 agtttaaaaa atacgtttgc gcaaacgtta agtggtcaag tgaaagcccataatttcaac 360 gcgacagcgc atcattacaa aaatgcgcgt gctgccgcta tggcaagtaatcacattccg 420 gaaagtgtgt acacgacgct tatcgaccag gttaacaccc acctgccgcttttacatcgt 480 tatgtcgcct tgcgtaaaaa ggtgttggca gtcgatcagt tacacatgtatgacatttac 540 acaccactga ctggccagcc gccgttgact tatacgcttg aacaagccaaagcagaggcc 600 ttaaaagctt tagccccact aggcgatgat tatcttgagc atgttcgcgaaatttttgac 660 aatcgctata ttgatgttgt cgaaaataaa ggcaaacgtt caggtgcctattccggtggt 720 gcttatgata ccaatccgtt catcttattg aactggcacg atgccgttgatgaactctat 780 acgttggttc acgaaaccgg ccacagcgtc cacagttggt acacgcgtcacaatcagccg 840 tatgtctatg gtgattatcc gatctttgtt gccgaaattg cttcaacaaccaacgaaaac 900 ctgttaactg attatttcct gacacattcg gatgatccca aagtacgagcctacattttg 960 aattactatc tcgatggttt taaaggaact gttttccgtc agacacaatttgccgagttc 1020 gagcactgga ttcaccaaca ggatcagcaa ggcgaaccgt tgacggccaccagcatgtca 1080 caatattacg ccgatctcaa cgcccggtat tatggaccgg aagttgcacgcgatccggaa 1140 attgcctttg aatgggcccg cattccgcac ttctattaca attactatgtttaccagtat 1200 gcaaccggct ttgctgctgc ctcaacctta gcagcgggca tcagtagcggtgaacctgac 1260 gcggctgccc attacttaga ctatttgaag tcagggagtt ctaagtatgccattgacacc 1320 atgaaaacag ctggcgttga tatgaccaaa cccgattatc ttgaagccgccttttcagta 1380 tttgaacagc gcttgacgga attggaaaaa atcttgcaga aagga 1425 31104 DNA Lactobacillus rhamnosus 3 atgtttaaac ccaccattca tcaacttcatccctatacgc cagaaaagcc tcttgccgta 60 ttaaaagaag aacttggctt gccacagctggtgcggatgt cagcaaacga aaacccattc 120 ggtaccagcg tcaaagttca gcaggccgtgaccaactgga attttacgca aagtcgtgat 180 taccccgatg gctatgccag tcaactacgcaccgcggtgg caaaacattt agacgttgcc 240 gcagagcagt tggtttttgg caatggtctggatgaagtca ttgccttaat tgcccgcact 300 tttttgagcc cgggggatga agtcattgaaccatggccaa cattttccga gtaccgcttg 360 catgcccaaa ttgaaggggc caccgtgattgatgtgcccg tcactgaaac tggcaatttt 420 gatttatctg caatggcgca ggcgctaaccgcgaaaacga aactgatttg ggtgtgcaac 480 ccaaataacc ccacgggcac gctgctgtcaattgcgacac tgaccgaatg gctgcgacag 540 ataccaaaag acgtgctggt tttaatggatgaggcttata ttgagttcac tgatgactat 600 ccagccacga gcgctatcag cttattatcaaagtttccaa acctcgtcgt gctgcgaaca 660 ttttcaaaaa tctatggact ggcgaatttccgggtcggct tcggtgtttt tcctaaacaa 720 cttgttaact acttgcaaac cgttcggctgccttacaatt taagcagcat tgcccaagtt 780 agcgcacagg cggccttggc tgatcaagattttgtcgcga tgacacgcaa gcgagtgcag 840 caagcgcgcg atagttggga acgctttttaacccaaactg gactgccaca cacccggagc 900 caaaccaact ttcaattctt tcaggccccaaaaatgcagg catcggcttt aaaaaagcgc 960 ctgctacaac aaggttttct tgtccgtgatggcttaaaac ccggctggct gcgcgtcacg 1020 tttggcactg aggtacaaaa cacggcggtacagcgcatca ttgaaacttt tcaggcagaa 1080 ctcactgggc caaatgcgct gaag 1104 41107 DNA Lactobacillus rhamnosus 4 gtgcatttag caaaaagaat cctcaacgtcgcaccgtcag cgacattggc cttaagtaat 60 cagacgaaag acttaaaggc aaaaggtgccgacgtcattg atttgtctat tggccaacca 120 gatttttcaa cccctaaggc gattgatgacgcagctattg cggcgattca ggctggtaat 180 gccagtttct atacggcagc aaccggtattccggaattaa agcaggcgat tagtgaccgg 240 atatttgccc aagacggtat tcgttatgatcatcgtcaaa tcgttgcaac caccggcgct 300 aagtttgctt tgtatgcctt atttcaggttttcttaaacc caggcgatga ggtgctgatt 360 cctgttccat actgggtttc ctacgaggaacagattaaat tggcgagcgg cgtgccacat 420 ctggtcatgc cggcagtcgg acataaagtcagtgtcgatg atcttgaggc ggctcggacc 480 gataaaaccc gggcattgat tatcaattcgccacaaaacc caagtggcgt tgtctatgat 540 cgcacggaac tgaccttaat tggcaattgggcgctgaagc atcatatttt ggtagtgact 600 gacgatattt accgagatct gatttataacggtacgactt acacctcaat gattagtatc 660 gatcccgata tcgcagcgaa tactgttttaatttccggcg tctccaagtc atatgcgatg 720 acgggttggc ggattggtta tgcggccggtccggaaaagc tgattcaggc catggcgacc 780 tttattagcc acacgacctc taatccggcagcagtttccg aatacgccgc ggtggcagct 840 ttaactggcg atcagcaggt tgttgaaaagatgcgccgtg cttttgaaga acggctgaat 900 cttttctatg atcttctggc agatattcccggtttcgata tgggagataa accgcaaggc 960 gccttctatc ttttcccgaa tattaagcgtgccgctcaat tgagtcatta tggtacggtt 1020 gatgatttta tcagtgcact gttgaccgaaaccggggttg ccattgttcc tggacgggcg 1080 tttgggcatg ccggatcatg cgcggat 11075 1170 DNA Lactobacillus rhamnosus 5 atgacattgc aacctttaaa cgaacaactacctgccatcg aggttagtga gattcgacaa 60 tttgacgaaa gtgtcagtga tattcccggtattttgaaac tgacgctagg cgaacctgat 120 ttcaacaccc cggaacatgt taagcaagccgggatcaaag ccattcagga aaattactcg 180 cattataccg ggatggttgg tgatccggagttacgcgaag ccgcacaaca tttttttaaa 240 acgaaatatg ccactgacta tcgggctacagatgaaattc tggtgacagt cggggccact 300 gaagcactgg caaccgccat tacgacgatcagtgatccgg gtgatgccat gctggttccg 360 tcaccaattt atccgggcta cattccgcttctgacgctga atcacgttac gccgctttat 420 atggatacga gtaaaaccga ctttgtcttgacccccgaac tcattgaggc caccatcact 480 gcaaatcctg acgctaaaat caaaggcattatccttaact atccaagtaa tcccaccggt 540 gtcacgtatc gggcggcaga agttaaagccattgcggaca tcgccgctaa acataacctc 600 tacattatct gtgacgaaat ttattctgaactgacttatg gtgagccgca tgtttccatg 660 ggacaatttg cctacgatcg tacatttattgtcaacggtc tgtctaaatc acatgcaatg 720 accggctggc gaatcggctt tttgatgggtccccagcagt taatcgcgca agccaaaaag 780 gtgcaccaat atcttgtgac tgccgcaacgaccattgccc agcgcgctgg tattgaagct 840 ctgacgaacg gtgcagacga tgctcaggtgatgaaagcag cttacgttaa acgccgtgat 900 tttgtttatg ccgccctcat cgacatgggctttagcgtgg ctcgtcctga tggtgccttt 960 tatctttttg caaaaattcc gacccaactgcatctaagct cacgcgaatt tacgcacgcc 1020 ttggcacatg aacagaagtt agctctgatttcaggtaccg cttttggccc cggcggcgaa 1080 ggttatatcc gaatcagtta cgcggcatcaatgaccgatc ttcaagaagc cgttaagcga 1140 ttgcgcgcgt tcatggccag ccacatcggc1170 6 891 DNA Lactobacillus rhamnosus 6 gtggcgcgcc tcatgcttgatcccggcgat ggcctagttg ttgaagcgcc aacgtatctc 60 ggtgccttag ccgcctttaatgcttatcag ccaacttatt atgaaatccc gatgcaagac 120 gatgggatgg acattaatgccttacagcgc gttttaatga gtcacaaagt caagttcatc 180 tatacggtac ctgattttcaaaatccaacc ggcgtcgtaa tgtctgtggc taagcgtcag 240 gcgctgattc gactcgccaaccaatatgac gttatgatcc tcgaagacaa cccctaccgc 300 gatcttcgct atgatggtaaaccgctgcca accattaagt catttgacac gcaaggccgc 360 gtcgtttatc tcggcagcttcagcaagatc ctctcaccaa gtctacggat gggctggctc 420 gttgctgcac cggaccttttgcaggaatta ctagcgttaa aaggcggaag cgacttggaa 480 tccagcaacc tgaccatgcacggcattgat gcctacatgg cggaaaatga cttagacgcc 540 cacatcaccg aaatccagaattgttgccgc gaaaagaaga atgccatggt cgcagcgatg 600 aatcgttacc ttcctgatgaagcgcacttc accaaccctg atggcggctt cttcctgtgg 660 ctcaccatgc cagccggcttcgacatgggt gccttcatga agcaacatct gttaccggaa 720 agcaacattt cctatgtgccttccgccaac ctatatgcaa cttcggctca ggtcaacggc 780 gcacggctaa acttcaccggtccgacactt gaacagatcg acactggtat caaagcatta 840 ggcgatgcgc tcaaaaccgcgctgcagcat cacctagtag ccgaacaagc t 891 7 1170 DNA Lactobacillusrhamnosus 7 atgaaattga caatttatga ctttgatcat gttatcgatc gccggggtacgtttagcact 60 caatgggatt atattgctga taggtttggc cgtaacgata tcctgcccttttcgatctcc 120 gatacagatt ttccagtacc agttgaagtg caagatgcgc taaaagaacggttaacacat 180 ccaatttatg gctatacacg atggaatcat gctacttaca aagacagtattgttcactgg 240 ttcgagcgtg atggtcatac aaagataaac ccggattgga ttgtttatagccctagcgtt 300 gtttttacga ttgctacact cattcgaatg aagagcgatc ccggggacggagtggctgtg 360 tttacgccta tgtatgatgc cttctatggt acgattaaac agaacgatcgagtgttgatc 420 ccgattcgat tagcagctgc agatgaaggc tatgtgattg attgggatagtttggcaacg 480 gtacttgctg aaaagcagac aaaaatattc ttactaacaa atccgcataacccgacagga 540 catgttttta caaaatcgga attagcacgc ctttatgact tgtgtcaggcagcccatgtt 600 ttcttgatct ctgatgatat tcaccgcgat attgtttatc cgggtcattcgtacgaacca 660 atgacaaatg tcggcacaag tgatgttgca ctctgctgct cagggtcaaagacatttaac 720 acaccaggcc tgattggctc atatgccttc ttaccagatc atgatgtaagggcacaattt 780 ttgacggaat taaagcagaa aaatgctctg tcttctgtaa gcatctttggcatgctggcg 840 caaattgcgg cttataacgg ttcagaggat tacgtggaac aactgactgcctatacaaaa 900 aataatatgg agttggttgc tagttattta gaggaaaatt tgccggaattgcagttttcg 960 ttaccggatg ccacgtactt agcctggata aatgtgtcta aactgagattaacgtcagag 1020 gaacttcaac atcggttagt aaacggcggc catgttggca ttatggcgggcaaaacttat 1080 ggtgatacca gatatctaag gatgaatatt gcctgtccaa agaagaagttagtgatgggg 1140 ctagaacgtt taaagaaggg aattagggga 1170 8 1158 DNALactobacillus rhamnosus misc_feature (1)...(1158) n = A,T,C or G 8atgatttatt ttgataatag tgcaacgacg aagatttcgc ctgatgcgtt ggcgacttat 60aacaaggtca gcacggattt ttttgggaat cccagcagtt tgcatgctct aggaactaaa 120gcaaatgagg ttttgcagag ttcgcgagcc cagattgcta aattaatcgg tgctaagccg 180gacgagattt attttacgag cgggggaact gaaagggata actgggtgan nttaaagggc 240actgcatggc ttaacgcgaa tttggcccgc attctgatta cgaccagtat cgagcctccg 300gctgtgatca atacgatgaa acagctagag aaactgggat ttgaagtgac ttatttgccg 360gttgatcggc gcggttttat tcatattgac gatttgaaag cggctattcg caaagatacg 420attttggtgt cgattatggc ggttaataat gaaattggca gtatgcagcc gattgttcag 480gccgcgcggg tgttggataa ttatccgaat attcattttc atgtcgatgc tgtacaagcc 540gttggtaagg ggttggatgc agcgttgcag gatccgcgga ttgattttct cagtttttcc 600ggccataagt ttcatgctcc ccgcggtacc ggctttatct atgccaaaga gggtcgcatg 660cttgatccgt tgctaaccgg tggcggtcag gaacatgatt ggcgctcagg cacggaaaat 720gttccggcga ttgcggcgat ggccaagtcg ctgcgcttac ttttggctaa tgaagatgct 780aatgtggccc ggcagcaagc agttcgcaag cggatttttg aacatgtcag ccaaaagccc 840aaggtgacga tgtttagtca gttaacaccg gattttgccc cacatgtttt atgttttgcc 900attgctggtg tccgcggcga aacgattgtt catgcgtttg aggatcatca gatttacatt 960tccacaacca gtgcctgctc gagtaagaaa ggcacggaaa gcagtacctt ggccgccatg 1020catacggacc cgaaaattgc tacttccgcg attcgggtga gtttggatga agctaatact 1080ttggatgaag cggatgcgtt taatgcagcg tttgatacga tttatgcaaa gtttgccaag 1140cttgataaag cgaccgtc 1158 9 786 DNA Lactobacillus rhamnosus 9 atgccaactaaaataggcct tcactacaac aaaattggag ttgggaaaac catctacttt 60 ttacatggcatggggttaga cggacacagc atggcagctt tttatgagcc acgttttacc 120 agcgaagagcggcattttgc ccgcctctat ccggatttac cgggaatggg aaattcaccg 180 gccacgtcagcattgcaatc tgctgatgat gtgttggcac aggttcatgc tttcattcag 240 gcgaccagcgaagggccatg ttatcttgtc ggccattctt acggtggcta tctagcatta 300 ggcctgcttgcacgatttcc tgacgagttt tccggtgctt ttttaaccgc tccggttgta 360 ctcgcggagaaaacagcacg tacggttgca acactaaagc atcttattag tgcgccagtg 420 accagtcagtcaccggaatt taccgactat caacacatga atgttgttat caatccttca 480 acctggcgacaatatcagga acttatcctg ccagggctta aaacttttaa ccgcgatttc 540 tgggttgccatgaagaaccg ccatgcttat cgtctgtcga ttgaatcacg cttaaccagc 600 ctgattaagtcaccagttac gcttgtgtta ggtgaaaatg acaatgaagt tggctatcag 660 gatcaagtggtctttgccca taaaggcgca cacatgacca caaccgtaat cccaaacgcc 720 ggtcataatctgatgatcga tgcgcctgag gctgtcatga ccgcgtttca tcagtttcta 780 cacaaa 786 10927 DNA Lactobacillus rhamnosus 10 atggtaacag cagcagataa tattacaggtttaattggca atacgccgct actcaagctc 60 aatcgcgttg tacctgaagg cgccgcggatgtttatgtca agctggaatt ctttaatccc 120 ggcggctcag tcaaggaccg gattgccttggcgatgattg aagacgctga atataaaggg 180 gtcttgaagc caggcggcac cattgttgagccaacgtccg gcaacaccgg cattggactg 240 gcactggttg cggcggcaaa aggttatcacctcatcatca ccatgccgga aacgatgagt 300 gttgagcggc gtgctttgat gcgtggttacggagccgaac tcattttgac gccgggtgcc 360 gatggaatgc cgggagcaat taaaaaagcagaagcattaa gcaaggaaaa tggctacttc 420 ttgccaatgc aattccagaa ccccgccaatccagacgtcc acgagcgcac gaccggacaa 480 gaaatcatcc gttcatttga tggtggcaccccagatgcct ttgtagccgg cgtcggcaca 540 ggcggaacac tcaccggggt tggtcgggctctgcgtaaga tcaatccaga tgtacaaatc 600 tatgcgttgg aagcagcgga gtcgccaatgctaaaagaag gccatggcgg caagcacaag 660 attcaaggga tctcagccgg ttttattccagacgtcttag atacgaacct ctatcaagac 720 atcattgaag tcaccagcga tcaagctatcgacatggctc gccacgtcag ccatgaagaa 780 ggcttcctac caggcatttc cgctggcgctaacatttttg gcgcgattga aatcgccaag 840 aaactcggca aaggcaagag tgtcgccactgtagcaccgg ataatggtga acggtatttg 900 tcgacggatt tgtttaagtt tgatgat 92711 810 DNA Lactobacillus rhamnosus 11 atgttaaaga aaaagttgtg gttcctgttgccgcttgtgg ccttggtaac cttcacgctc 60 accgcttgca ccagcgcatc atctgacacgtcaaaaaaca gcgacgtcac cgccgaactc 120 atcaacaaaa atgagcttac catcggccttgaaggtactt atgcgccatt ttcttatcgc 180 aaagatggca aacttgaagg cttcgaagtggaactgggga aagccttagc caagaaaatc 240 ggggttaagg caaaattcgt gcccacccaatgggattcgc tgattgcagg attaggcagc 300 cagaaatttg atctcgtact gaatgatattagtgaaacgc ccgcacgcaa aaaggtctac 360 aacttcacca ctccgtacat gtactcgcgttatgccttaa taacccgcag cgataacacc 420 accatcaaat cgcttgccga tattaaaggcaaaacatttg tcgaaggcac cggtacaccc 480 aatgccgctt tagccaaaaa atacggcgctaagatcaccc cgtctggcga ctttaccgta 540 tcgcttagcc ttgtgaaaga aaaacgcgcagacggaacca tcaacgcctc ggctgcatgg 600 tatgcctttg ccaagaataa ctcaaccgcgggcttaaaga gtcaaaccct caaagatagt 660 gtcgttaaac ccgatgaagt agctggcatggtcagcaaaa aatcgcctaa actacaagcc 720 gcactttcaa agggcattca agaactacgcaaagacggca cgttgaaaaa actgtcgcaa 780 aaatattttg gcaccgattt aaccaccaag810 12 1425 DNA Lactobacillus rhamnosus 12 ctgatttgca aaggtcgaagcttgaagcca ttcggacatt ttattgatgc catcacggta 60 aatcgagaac acgtcttgacaaccgctgcc gaggccttga ttgcaagtgc gggcgatgcc 120 ttaaatgcca gtcacgcgaccttcaatgta ttaaacaact ctgatctgca attcgggttt 180 gttgaaaatg aagatggcgaaaccgtccag cttagtaacg gtttgtacgg tcagttgatt 240 cgctcgacta accgtaaattacgcaaggag gctttcgagg cccttctgcg tgcttacgaa 300 agtttaaaaa atacgtttgcgcaaacgtta agtggtcaag tgaaagccca taatttcaac 360 gcgacagcgc atcattacaaaaatgcgcgt gctgccgcta tggcaagtaa tcacattccg 420 gaaagtgtgt acacgacgcttatcgaccag gttaacaccc acctgccgct tttacatcgt 480 tatgtcgcct tgcgtaaaaaggtgttggca gtcgatcagt tacacatgta tgacatttac 540 acaccactga ctggccagccgccgttgact tatacgcttg aacaagccaa agcagaggcc 600 ttaaaagctt tagccccactaggcgatgat tatcttgagc atgttcgcga aatttttgac 660 aatcgctata ttgatgttgtcgaaaataaa ggcaaacgtt caggtgccta ttccggtggt 720 gcttatgata ccaatccgttcatcttattg aactggcacg atgccgttga tgaactctat 780 acgttggttc acgaaaccggccacagcgtc cacagttggt acacgcgtca caatcagccg 840 tatgtctatg gtgattatccgatctttgtt gccgaaattg cttcaacaac caacgaaaac 900 ctgttaactg attatttcctgacacattcg gatgatccca aagtacgagc ctacattttg 960 aattactatc tcgatggttttaaaggaact gttttccgtc agacacaatt tgccgagttc 1020 gagcactgga ttcaccaacaggatcagcaa ggcgaaccgt tgacggccac cagcatgtca 1080 caatattacg ccgatctcaacgcccggtat tatggaccgg aagttgcacg cgatccggaa 1140 attgcctttg aatgggcccgcattccgcac ttctattaca attactatgt ttaccagtat 1200 gcaaccggct ttgctgctgcctcaacctta gcagcgggca tcagtagcgg tgaacctgac 1260 gcggctgccc attacttagactatttgaag tcagggagtt ctaagtatgc cattgacacc 1320 atgaaaacag ctggcgttgatatgaccaaa cccgattatc ttgaagccgc cttttcagta 1380 tttgaacagc gcttgacggaattggaaaaa atcttgcaga aagga 1425 13 768 DNA Lactobacillus rhamnosus 13agttatgcgc caaccatcac actagaacaa gccaaagaag atattaaaaa tgccaccgca 60ctcatgggtc aagattacca ggcacagatg atgcaggcct tttcagagcg gtggatcgat 120tttcctgcta atcaaggcaa ggattccgga gcttacactg ctggaccgta tggtgtgcac 180ccttatgtcg aaatgacttg gagtaatacg ctgcctgctg tttacacctt gattcacgaa 240ttaggccata ccgctcagat ggttcgttca caagaagccc acaatgtgtt ggatgcggac 300tttaatgcct atttggtcga aagtccttcc accttcaacg aactgttgct gactcactat 360ctcgaagaaa acgctaaaga tccgcgaatg aagcgctttg ccttgtcacg gttattaaat 420gatacctatt tccacaactt tgtcacccat ctgctcgaag ctgcgtttca acgggaagtc 480tataacttga tcgataacgg cgaaactttt gatgctgctc ggttgaacgc cattacgcgt 540aaagtcctga ccgatttttg gggatcggca gtagaacttg agccaggtgc cgagctaact 600tggatgcggc aaagccatta ctacatgggc ttatattcgt actcctattc ggccggcttg 660acggttgcta cccaagcgtt tcaggccatc gaacaacaag gtcaaccagc cgttgatcgg 720tggttgcgtt atctcagcct aggcgactca cttgatccag tcgaagca 768 14 1923 DNALactobacillus rhamnosus 14 ttgttaggtc agtttggtgt tgatctcact gaacaggcacgcaaaggtca aattgatcca 60 gtcatcggtc gtgataagga aatttcacgc gtgattgaaattctgaatcg ccggaccaag 120 aataatccag ttttaatcgg tgaagccggg gtcggcaaaaccgcggttgt tgagggactg 180 gccttaaaga ttgctaatgg cgacgttcca gccaagttgcaggatcgcca tgtgattcgc 240 cttgatgtcg tttcactcgt tcaaggcaca ggcattcgcggtcaattcga gcagcgcatg 300 caacaattga ttgacgaact gaagcaaaat aagaatattatcctatttat tgatgaaatc 360 cacgaaattg tgggtgccgg caatgctgaa ggcgggatggacgccggcaa cgttttgaaa 420 cccgcattgg ctcgtggcga attacaacta gtcggcgcaacgaccagcaa cgaataccgt 480 cagattgaaa aggattctgc cctcgctcgg cgccttcagccggtgatggt tgaagagcct 540 agcgttgatg aaaccattaa aattctgaaa ggtctgcaaccgcgctacca agacttccac 600 catgtgaaat acacggaagg cgcaattgaa gctgcggcaaccctcagcaa tcgttacatt 660 caggatcgtt tcctccctga taaagccatt gacttgttagacgaagccgg ttcacgcaag 720 aacctaacga ttgccaccgt ggatcctgaa accattaaagctaagattgc tgatgccgaa 780 aagcaaaagc aagccgcact caagcaggaa gactatgaaaaagccgcctt ctatcgtgat 840 caggtcacca agttagaaga catggccaaa aagcaatccaacctgccaga taatgaaatc 900 ccaacagtta ccgaaaaaga catggaaaaa attgttgaagaaaagacaaa cattccagtc 960 ggtgaactca aagctcagga acaggcgcaa ctgaagaatctcgccagtga ccttgagcaa 1020 cacgtcattg gtcaaaatga agcagttgat aaagttgctcgggcaattcg gcgcaatcgt 1080 atcggcttca ataaaaccgg gcggccaatt ggctcattcctctttgtcgg accaaccggt 1140 gtcggcaaaa cggaactggc aaaacagctc gctaaagaactattcggttc tgaagatgcc 1200 atgattcggt ttgacatgtc ggaatacatg gagaagttcagcgtctctaa gctcatcggg 1260 tcaccgccag gctatgtcgg ctatgaagaa gccggccagctaactgaaaa agttcggcgc 1320 aatccataca gtttgatttt gcttgatgaa attgaaaaagcccacccgga tgtcatgaac 1380 atgttcctgc agattctgga tgacggccgc ctaaccgattcacaaggtcg aactgtttcc 1440 ttcaaagata ctatcatcat catgacttct aacgccggatcaactgatgc ggaagctaac 1500 gtaggctttg gtgcaacgtt aagcggtaaa acccacagtgtgctggatca gctgggtaac 1560 tacttcaaac cagaattcct gaatcgcttt gatgacattgttgaattcaa gccgctttct 1620 aaagacgacc ttttgaagat tgtgtcactg atgattaatgacactaacaa caatctcaag 1680 agtcagggat taacgattca cgtcaccgat cccgtcaaagaaaagcttgt cactctgggt 1740 tacaatccat ccatgggggc acggccattg cgtcgggttatccaggaaca gattgaagac 1800 cgtgtggctg acttttacct cgaccatcct aatgccaaggaacttgaagc aaggatcagc 1860 aacggagaaa tcacagttgg cgaaccagcc aaggcagaagcctcttcaaa aacagccaag 1920 aaa 1923 15 1443 DNA Lactobacillus rhamnosus15 accaagtcag ttgtcggtgt agcgccagaa tcacaattat tagcgatgaa ggtgtttacc 60aattcagaca catcagcgac gactggttcg tcaacgcttg tttctgcgat tgaagattca 120gccaaactgg gcgcggatgt tttgaatatg tcccttgggt ctgtttccgg caatcaaaca 180ctggaagatc ctgaaattgc agccgttcaa aacgccaatg aatccgggac cgcagcggtt 240atttcagcag gaaattcggg tacatccggg tcaggtacag aaggggtcaa caaagattat 300tatggcctgc aggataatga gacggttggc acaccgggga catcacgcgg ggcaacaact 360gtcgcatcgg ccgagaatac agatgtcatc aaccaagctg ttacgattac tgacggcagt 420gggttaaaac tcgggcctga aaccgtacag ctttcaagca atgactttgt tgacagtttt 480gatcagaaga aattctacgt tgtcaaagac gcaagcggta agttaagtac aggtgatgct 540ggcgactata cggcggatgc caaagggaaa attgcgattg tcaaacgtgg cagtctgact 600ttcactgaca agcagaaata tgccgaggcc gctggtgcag caggcttaat cattgttaat 660aatgatggca catccacacc tttgacttcg atttctctaa cggctacttt tcctactttt 720ggtctttcca atacgactgg ccaaaaactg gttgattggg taactgcgca tccaaatgac 780agtctgggcg taaaaattgc cttggcattg ttgccaaatc aaaattataa agcagatcgg 840atgtcaagtt tcacttcata tggccctgta tctgatcttt cctttaagcc tgatattaca 900gcgccgggtg gtaatatttg gtcaacgcaa aacaacaatg gttatacgaa tatgtcgggg 960acctcaatgg catctccatt tatcgccggc tcccaggcac ttctaaaaca agcgctaaat 1020aataaagaca atgagttcta tgccgattac aagcaactta aaggcacagc attaaccgat 1080tttctcaaaa cagttgaaat gaatactgca aagccgatta atgatattaa ctatgataat 1140gtcatcgtct ctccacgccg acagggagca gggttggtgg atgtcaaagc cgcgattgat 1200gctttggaga agaatccatc aacggttgtt tcggaaaacg gctatcctgc tgttgaattg 1260aaagatttca caagcactac caaaacgttt aagttgacgt ttaccaaccg caccaaacat 1320cagctgacgt atcaaatgac tagcaatgaa gataccaatg cggtttatac ttcggctacc 1380gatctagaat cgtttataca aagcagtaaa atggctaaac taattcatga aaggggcgcg 1440gca 1443 16 993 DNA Lactobacillus rhamnosus 16 atgaccatta attggcagcaagaagttgaa aaattggaac cccaacttct ctcagatcta 60 accacacttt tgaagatcaattcggaacgt gatactgatc atcaaaccga tgagtatcca 120 ctcggaccgg gaccggccaaagcgcttgaa gcatttttgg cgattgcaca gcgggacggt 180 ttcaaaacat taaatgtcgaccatgttgcc ggccgcatcg aattaggcga tggcgatgaa 240 atctttgggc tttttggccatgttgatgtc gtgcccgcgg gaccaggctg gcaaaccgat 300 ccatttgacc ccgttattcgcgatggcaag atttatggcc gcggaacaag cgatgacaaa 360 ggcccaagta tcgctgcttactatgcctta aagcttattc gcgatctcaa gttgccgatt 420 aataaaaaga ttcacttcattcttggtacc gatgaagagt ctgactgggt cggtattcac 480 cgctatctcg aaactgaacctgctcccgac ttcggatttt caccagacgc ggaatttcct 540 atcatcaatg gcgaaaaagggattgctagt tttgaaatcg ttcaaaaacc aatcgccgct 600 gcaaccgctg atctaacgttgaatcatttt tccgccggta ttcggccaaa catggtgcca 660 caagaagcaa aggctgtcctcagcgggcca ttaccggaag catttgtgac gcaagccgag 720 aaatgggcag cggagcaagaagtcaccctc actctgacac taggcaaccc gacaacgatt 780 gaattgattg gaaaaggcgcccatgcccaa gaaccaaaag atggcaaaaa cgccgcaacc 840 tatttagcaa cgcttttggccgacttacca tttgatccag ccgggaaagc ctatctgacc 900 atgattgcca accaccttcatctggactca cgtggtcacc atttagggat taattatacc 960 gataaactaa tgggtgacctgaccgcaagt ccg 993 17 1032 DNA Lactobacillus rhamnosus 17 ggcaaaatgagtctgtatgc tggcgggcct gacgagcggt tgacgccttt gattgatggt 60 cggcgtcatgtgacggactt tgcattgaca ccagatcatc gtggggtggt tttcactgag 120 agtacgatgaccattccgag tcggctggtt tattttgatt tggcatcgga agaagagcag 180 gttttgtatgacccgaatcg tcaggtaaca cgtcacttgg gcttagttac ccctcaaacg 240 tttaattttcaacgagacgg ttttgagatt gagggctggt attttccacc gcaacaggcg 300 tcatcatcgcatccggcaat tttgtatgtc catggcggcc cagcagtcgg atatggctat 360 acctttttccatgaaatgca gtatctggca gcaaaaggct atggcgtgat ttgtcgaaat 420 ccgcgtggagggttaggtta ccgcgaggca tttacgggcg ctgtcattaa acattaaccg 480 gcaggcgattatgaagattg cttggcttcg ggtgaagaag cgctaaagct cgatacaaca 540 attgatccgcaacgtctatt tgtcactggc ggttcttatg gcgggtttat gactaactgg 600 attgtgacccatacgcatcg ttttaaagca gcggtaaccc agcgttcgat ttccaattgg 660 ctgagtatgtatggtaccag tgacatcggt tattacttta caccgtggga actagaagga 720 aagtggactggcgatttgtc agatgtgcaa gggctttggg atttttcacc attagctcac 780 attgatcatgccagaacacc gacgcttgtg atgcacagtg aaaatgatga acgctgcccc 840 atcggcccaagtagaaaagt tgatcatcgg tctcaaactg catggtgttg aaaccaagtt 900 catgcgtttcccaaagtcaa atcatgattt gtccccgcag cggggttgcc gaatttgcga 960 gtggcacgattgcaggcaat tgtggattgg tttgacgccc atcaagcaca accgcagatg 1020 gctaaaggagaa 1032 18 1674 DNA Lactobacillus rhamnosus 18 catttaatcg gtgcgacgacgctggacgaa tatcgcgaaa atattgaaaa agataaagca 60 ttagagcgcc gattccaacgggtgctggta caagagccaa ctgtggaaga tacgatcagt 120 attttacgtg gcttgaaggaacggtttgaa atttttcaca aagtgcgcat tcatgattcg 180 gcgttggtgg ctgccgcgacattatccaat cgctatatca cggatcggtt tttaccggat 240 aaggcgattg atttagtcgatgaagcctgt gccacgatta atgttgaaat gaactcgcgc 300 ccaactgaac tggacgtggccgagcgtaag cagatgcagc ttgaaatcga gcagcaggcg 360 ttaaagaatg aaagtgatcccgcaagtaag aaacgcctgg aaaatgcaaa cgccgaattg 420 gcaaatttaa aagaaaaaaccaataaactc aaagcacagt gggaagctga aaagaaggac 480 attcgccagc ttaacgagaagaagtcagcg atcgacaaag ctaaacacga actggaagat 540 gcccagagcc gttacgatttggaaacggct gctcgtctgc aacacggaac gattccacaa 600 cttgaaaaag aattgcaggcaatggagcac agtgatcggc cgcagtcttg gctggtccaa 660 gaaagtgtca cggctaatgagattgctgct gttatttcac gagaaaccgg tattcccgtg 720 gcaaaactgg ttgaaggcgatcgtcaaaaa ttgctgcatc ttgccggtaa tctgcatcag 780 cgtgtcattg gtcaggatgaagccgtcacg gcagtttcag atgcggtatt gcgttcgcgc 840 gccggactgc aagacccaagccggccatta gggagttttc ttttccttgg tccgaccggg 900 gtggggaaga ccgaactcgcaaaagcgctg gccgaggatc tgtttgattc tgaaaaacac 960 atggttcgaa ttgatatgtccgaatatatg gagaaggcga gtgtttcacg gttggtcggt 1020 gcggctccgg gttatgtcggttatgaacaa ggcggtcaat taaccgaagc agttcgccgc 1080 aatccgtata cgattgtcctattagacgaa atcgaaaaag ccaatccgga tgtcttcaac 1140 attcttttgc aagtgttagacgatgggcgg ttaacggacg ggcaaggacg caccgttgac 1200 tttaagaaca cgatcatcattatgacctct aacctcggct ctgaatattt gctggatggc 1260 gtgcaaaaag acggaactgtcagtcagcaa gctaaggatc aggttcgtca gttgatcggt 1320 aaagctttta aaccggaatttctcaatcgc attgacgata tcatcatgtt ccatccactt 1380 tcactagatg atgttaagaagattgccgtt aaggatctgc atgagctggg aacacgcttg 1440 gcagaccagc aaattagtttggacatcacg ccagaagctc agacctggtt ggcggataag 1500 ggctatgacc cggcatttggtgcgcgtccg ttgcagcgtc taattaccag tgccgttgaa 1560 acgccgttag ccaaagagctcattcgcgga acgattcagc ccggtcagga agtggtcata 1620 accgttgcgg atgatcagctgcaattcaaa gcaaaacaag tagtagcgaa ggca 1674 19 876 DNA Lactobacillusrhamnosus 19 atttcggcga tcatcgtgat tgtagaggag aataatgtgg cagcaagagaattaatttta 60 gcattcgaaa gcagctgcga tgaaaccagc gtggccgttg tcgaaaatgggaccaaaatc 120 ctatcgaaca tcatcgccac gcaaatcaag agtcatcagc ggtttggcggcgttgtaccg 180 gaagttgcca gtcgtcacca tgtggaacag attacgttgg tgaccgatgcggcattaaaa 240 gaggcaggtg tgacttatac tgacctgacc gcagttgccg tgacgtatggaccgggactg 300 gtaggtgcct tgttgatcgg ggtacgggct gccaaaccca ttgcgtatgcccatcactta 360 ccacttattc cggtcaatca tatggcaggc catatttatg cagctagatttgttaagcca 420 ttggtctatc cattgttggc attagcggtt tccggcgggc acacggaactggtctatatg 480 cgcgctgccg gtgaatttga aatcatcggt gatacccgtg acgatgcggccggtgaagcg 540 tatgacaaag tcggccggat attgggtatc ccttacccag ccggaaaagaagtcgataga 600 ttggcgcatc ttggtcatga tacctttcat tttccgcgag ccatggataaagaagacaat 660 ctcgatttta gtttcagcgg tttgaagtca gctgtcatta atacggtacatcatgccgat 720 caaattgggg aatcactcag ccgtgaagat ctgtctgcga gttctcaagcgtcagtggtg 780 cacgtgatgg ttctcaaatc ccaatcagcg atagccgaat atccggttatacaggtggtg 840 atcgccgggg gcgttgccga taatcaaggg ctgaaa 876 20 732 DNALactobacillus rhamnosus 20 atgattttcc gcaaaccaca accattcgaa tatgaaggtaccgatactgg cgtggtattg 60 ttacatgcat acacgggtag ccccaatgat atgaattttatggcgcgggc cttgcagcga 120 tccggttatg gggtttatgt tccgcttttt tccgggcatgggacagtgga gccgttagat 180 attttgacaa aaggcaaccc ggatatttgg tgggcagaaagtagtgccgc ggttgcgcat 240 atgaccgcaa aatacgccaa ggtgtttgtt tttggcttatcactgggagg tatttttgcg 300 atgaaggcgc tagaaacctt gccagggatt acagcaggcggtgttttttc atccccgatt 360 ttgccgggca aacatcactt agtaccgggt tttttaaagtatgccgagta tatgaatcgg 420 ttagcaggca aatcagatga aagcacacag attctggcatatttgccggg acagttggcc 480 gcaatcgatc agtttgccac gacggttgct gctgatttaaatttagtcaa acagccgact 540 tttattggac aagccggtca ggatgaatta gttgatggtcgattagcgta tcaattacgc 600 gatgccttaa tcaatgctgc acgcgttgat tttcattggtatgatgatgc caagcatgtc 660 attaccgtta actcggccca tcacgcatta gaagaagacgtaatcgcatt tatgcaacaa 720 gaaaacgagg ga 732 21 1299 DNA Lactobacillusrhamnosus 21 ctgggtatat ttttctttaa acgcttcagg aagttgcact tgttcgatcctttaaattat 60 ccggaagaga cttttcaaag tttcgacagc gcttttaaca acggagctgattacgttgag 120 cttgacgtac atgaaagtgc agatggtgtg attgtgattc aacatgacaccacgattcag 180 cgaacgactg gtgccaactt ggcgatcgcg aaaacaaact tcgcacaacttcagcaatat 240 cataccaaaa atggcgaacc gattcacagc ctagaggaac tcttcgcccatgagcaacaa 300 acaaagcata aattcctgat tgaaactaaa attgtaaaag gtgaaccgcatccgcatcta 360 gaagacaaag ttgcggccct gatcaagcaa tatcacatgg aaaatcgcgtgatgtttcat 420 tcattctcag cagctagcct caaacgcttg caagcagctc tgccaaatattccgcgaatt 480 ttgatcgttg gctcgcttaa gcggatcaac tttgacgtct tgacgtacgtggacggtatt 540 aatctaagtt ccgatttagt gacgccgcaa cttgtcaccc aactgcatgatctaggtaag 600 aaagtttatg tttgggatga aatgaacgag gatcgggcga aatggacttggctcgtcaat 660 ctcaacattg atggcgtcgt cactaattac accagcctcg gccacgaatttcaaacgctt 720 aaggcagctg ctgtcaccac cagcatcaat gatcttggcg caaactcaagccttgctgca 780 ctgccagttt atgaaaatcc ttatcagcca ttgttgcgct ctgaacggctggcaccgcaa 840 accccgatca tgatttccag catggtttcc ctcgctggca gcacgtactaccaaattggc 900 gataatgcat ttgtccctgc cgaaaccatt aaccttgccc ctgaagccggttgggcaagt 960 ctttttctcc atcagcgcat cgtcatcacc agccggcact ttaaggtacccgtgcacgct 1020 gatcccttac atcagcaagc cattaccggc catgttggca atcacaaatgctaccgggta 1080 ttagcagccc gttatcaaag cggccagctg tatttaaaaa caaaaatcggttggttaaac 1140 gccaaagatt tacaggtgct gccaaccgcc gagaatatgc gcatctggctcacgctctat 1200 cgcagcatcc ccgaaaacca aaaaccgctc cttcactggg cacttggcgacacggccttc 1260 gatacaccgc ttctcaatgc cagtgtcctg aacatcggt 1299 22 1344DNA Lactobacillus rhamnosus 22 atggaattag cgaaactcgc tgttgacgaaaccggccgtg gtgtttggga agataaagcc 60 attaaaaata tgtttgccac tgaagaaatctggcattcga ttaagaacaa caagaccgtt 120 ggcgttatca atgaagataa acaacgcggcttggtatcca tcgccgaacc aatcggggtt 180 attgccgggg taacgccggt gaccaacccgacatcaacca cgatctttaa atccgaaatt 240 tccatcaaga cccgtaatcc gattatctttgctttccacc cgggtgcaca aaagtcttca 300 gcgcgtgcgt tggaggtcat tcgggaggaagctgaaaagg ccggattgcc aaaaggggcc 360 ttgcagtata ttccggttcc aagcatggaagcaactaaga cactgatgga tcatcccggc 420 attgccacga tcttggcaac cggtggccctggcatggtta agtcagctta ttcatccggc 480 aaaccggcct tgggtgttgg cgcagggaatgcaccggcat acatcgaagc aagtgccaat 540 attaagcaag ccgttaatga tttggtcttgtccaagagtt ttgataacgg tatgatctgt 600 gcttccgaac aaggggccat cgttgattccagcatttacg atgccgcgaa gaaagaattt 660 gaagcccaag gtgcctattt tgtcaaacctaaggacatga agaagttcga gagcacggtt 720 attaaccttg agaagcaaag tgttaatcctcgaattgttg gccaaagtcc taagcaaatt 780 gctgaatggg cagggattcg aattcctgatgacacgacca tcctgattgc cgaactaaaa 840 gacgttggca agaaatatcc gctttctcgggaaaaactga gcccggtttt ggcgatggtt 900 aaagccgatg gtcatgaaga tgccttcaagaaatgtgaaa ccatgttgga tatcggcggc 960 ttgggacaca ccgcggtgat tcacacagctgacgacgaat tggcattgaa atttgctgat 1020 accatgcagg cttgccgaat cctgatcaatacaccttctt ctgttggcgg tatcggggat 1080 ctctacaacg aaatgattcc tagtttgacgctgggctgcg gctcctatgg cggcaactcg 1140 atttcgcaca atgtggggac ggttgacttgttgaatatca agaccatggc aaaacggcgc 1200 aacaacatgc aatggatgaa attgccgccaaagatttatt tcgaaaaaaa ctcggttcgc 1260 tatctggaac acatggaaag catcaagcgcgccttcatcg ttgctgatcg ttcaatggaa 1320 aaagctgggt ttcgtcaaga tcat 1344 23474 DNA Lactobacillus rhamnosus 23 gtgttggtca ataatgccgg cattaccgacgatatgttgg cgatgcgcat gaaacccgct 60 tcttttgcca aggtcgttca ggttaacttggatggcacct tttatgttac ccaaccggcc 120 ttcaagaaaa tgctaaaggc ccgcgctggcgtcatcatca atctggccag tgtggtcggt 180 ttgaccggta atatcggcca agccaattatgcggcaagta aagcaggcat catcgggctg 240 actaagacgc tagctcgtga aggggctatgcgtggcgtgc gcgttaatgc cattgcccca 300 gggatgatcg ccaccgatat gaccgctgccttgagccaat ccagtcagga ccagattctg 360 gcggaaattc cgttgaagcg gttcggtcaacctgaagaaa ttgcccacac ggcccgtttt 420 ctggtcgaaa atgcctacat aaccggtcagacagtgactg tcgccggcgg atta 474 24 1002 DNA Lactobacillus rhamnosus 24atgtatcatg cagcagctga tcgttatgag aaaatgccgg ttcgccatgc tggtaagaca 60gggttgatgt tgccggttat ttcgttggga ttgtggcagc attatggcaa cttggatcca 120tttggcccgc gacgctcggt gattttggat gcgtttgatc gtggcgtttt tcattttgat 180gtcgctaatc attatggtaa tggtgatcgt gaaccgggat ttggctctag tgaaaggtta 240ctcgggcaga ttctggccac ggatttaaaa ccgtatcgag acgaattggt gattagtacc 300aaggtgggtt atgagattca ccctggtcca tacggtgtcg ggacgtcgcg taaagcagtt 360attcaaggct tgaatgattc actcaagcgc ttgcagttgg attatgtcga tatttactat 420gcccaccgat ttgacgatac cgtggccttg gaagagacgg ttaatgcgct ggatcaaacg 480gtgcgtgacg gtaaggcgtt gtatattggt atttccaact atgatacgaa gcagaccaaa 540gaagcaattg cgatgtttaa agatctgcac acgccttttg tactgaatca atacagttac 600aacatgttta atcgcaccgc tgaaacgtcc ggcttgatcg atgcattaaa agctgatggt 660gccgggttga ttgcatacgg accgttatca gaaggcttgt tatcagatcg ctacctaaag 720ggaattccgg atactttcaa aatccatcca accaacaagg ccacttttgc taagggcaaa 780gaggctgtgg ttaagcaact aaatgcgctt aatgaaattg cgcatgatcg tgaccaaacc 840ctgagtcaaa tggccttggc gtggttgtta cgggatccgg ttgtcacaag tgtgatcatt 900gggacgacct cagttgaaca ccttcaggat aaccttaaag caacggaaca tctgaccttt 960actgctgaag agattcaaca aattgatgat attttaaatg ct 1002 25 1239 DNALactobacillus rhamnosus 25 gccgttgcat taccgttact tggcgttttg gctatcgccgcaacgcatgc tgaaggtgtt 60 tatgatattg gccgtccact tggccgcttc ttcgccttagcgttcatggt gctcattcat 120 gccacgatcg ggccaatgtt cggcacgccg cggactgccaccgtttcctt caccaccggc 180 gtcttaccga tgttaccaaa agcctggcaa caaggcggcttgcttgtctt ttcggcttta 240 ttctttgggg ctgctttttt cctgtcatac aaggaacgcaaaattaccac agctgttggt 300 aaagtcttaa atcccgtctt cttgctattg ttattcttcgtcttcttcat tggtttctta 360 cacccaatgg ggaatccggc tgctcaaaca gtaacggctgcgtacaaaaa tggcggcagt 420 ttcatgagcg gcttcctgca aggttacaat accatggacgcgcttgctgc cttagccttt 480 ggtgtgactg tcgtaacggc ggttcgcggt ttaggcttgaaaaacgatga tcatgtcgcc 540 aaagcaacgg ctaaagctgg ggtcatggct acgagttggatcgcgttaat ctacgttgcc 600 ttgatcgtct taggaagtat gtccctggcc cactttaagcttagtgctga agggggaact 660 gcttttaatc aagtgggtac gttctacttt ggtactgttggccaccctgc ttggcaacct 720 tgcttaaccc tgacctgttt gaacaccccg gttggttttgtcagggcatt cccgcacgac 780 ttccaccggc atttccctaa agtcagctat caggtctggcttggattgac aagttttctg 840 tcattcttaa ccgccaactt cggacttgaa caaatcattgcatggtccgt tccaatgctg 900 atgttcctat atcctttctc aatggttctc atcttactatcggtctttgg caaagcgttc 960 catcacgatc cactagttta ccgaatcgtt gtagcattcaccatcgttcc ggctgtgctc 1020 gacatgtttg cagctttccc cgccgttgtt agtcaaagttcgttaggctt ggcattgcat 1080 agcttccagc ttcattttct accattttcc gcaatgggtctcggctggct cgtgccggct 1140 ggtgtgggtc tcgtccttgg cctcgtcgca catgccgtcaaagttcgcaa agcagtcgca 1200 gcaactcatc tcgaagctga acaaactcag ctagtacac1239 26 1881 DNA Lactobacillus rhamnosus 26 atggcagaca atcacaaagcccaaacgacc aagcagccat ctggcccacg gatgggtcct 60 ggccgtggtg gtctcgttgaaaagccgaag aacttctggg gcacaacagc gcggttattt 120 ggttacatgc gcaaccgtctgattggtatt attgcggtgc tagtcttggc cattgcttcg 180 accgtctttc aaattcgcacgccaaaaatc ctaggggaag ctacaaccga gatttttaaa 240 ggggttatga aaggccaagcggagcaaaag gccggtatcg ctgttggcaa ctatccaatt 300 gattttgata agatcaaacagattatttta atcgtcttgg tgctgtattt gggtagcgca 360 ctgtttagtt tcttgcagcagttcatcatg actcggatct cgcagaatac ggtttaccag 420 ttgcgtaaag atctcaagcacaagatgaag actgtgccga tcaagtatta tgacacgcat 480 agtaatggcg atattatgtcacgggcgatt aacgatatgg ataacatcgc ctcgacactg 540 caacaaagtc tgacgcagatggtgaccagt gcggttatgt tcgtgggaac catttggatg 600 atgcttacga tttcctggaagttaacgctg attgcgctgg taacgattcc gctgggctta 660 attgttgtcg ggattgtcgcgccaaaatcg caacggttct ttgccgccca gcaaaaagct 720 ttaggtctct tgaataatcaagttgaagaa acttatggcg gtcaggtgat catcaagagc 780 tttaatcgtg aagatgatgaagttgaggca tttgaaggcc agaaccaggc attttatgat 840 gcagcgtgga aagcgcagtttgtttccggg atcatcatgc cgcttatgat tttcctaaac 900 aacattggtt acgtgtttgttgcgattatg ggtggcattg aagtttccaa tggcacgatc 960 acccttggga atgttcaggcgttcctccag tatatgcagc aattttccca gccgatttcc 1020 cagcttgcta acctagccaatacgattcaa tccactattg ccagtgccga gcggatcttt 1080 gcggtgttgg acgaagaagatatgcaggat gagccgtctg gtgtgccggc agtggccaat 1140 gatcctaaca aactggtcatggatcatgtt cagtttggtt ataccccgga tgccttgttg 1200 ctcaaggact ataacctgcaagtcaaaccg ggtgagatgg tcgcgattgt cgggccgacc 1260 ggtgcaggga aaacaacgatcatcaacctg ctagagcgtt tttatgatat tagcggcgga 1320 tcgattcgct tgaatggtaccgatacccgc gatatgaagc gagaagatgt tcgcgcgcat 1380 tttgcgatgg tgcttcaggatacttggctg tttaccggca cgatttggga taacttgaaa 1440 tatggccgcg aagacgcaactgacgacgaa gtattggccg cagccaaagc agcccatgtt 1500 gacaactttg tgcggcagttgccggatggc tacaacacga ttctgaacga agaagcctcg 1560 aatatttcgc aaggtcagcgacagttgttg acgatcgctc gggccttcgt ggcagatccg 1620 gaaattctta ttctggacgaggccaccagc tcggttgata cgcggacgga aattcatatt 1680 caacacgcca tgaaccgtttgctgaccgat cgtacgagct tcgtagtcgc ccaccggctg 1740 tcaacgattc gtgatgccgacaagattatc gtgatgaatc acggctccat tgttgaaacc 1800 gggaatcatg acgaactaatggctaaaaac ggcttttatg ctgatctgta caacagtcag 1860 ttcagtggca atgtcgcgat t1881 27 606 DNA Lactobacillus rhamnosus 27 accaccaggc tgtcaagtttgatcaccgaa tacttagaca gccaactagc tgaacgtcgc 60 agcatgcatg gcgtcttggttgatatttac ggtctgggcg tgctcattac tggcgattcc 120 ggggtgggaa aatccgaaactgccttggaa ttggttcagc gtggtcatcg gcttattgct 180 gatgatcggg tggatgtttatcagcaagat gaacaaacgg ttgtcggagc tgcaccgccg 240 attttatccc acttgttggagattcgcgga ttgggtatca ttgatgtcat gaatctcttt 300 ggtgccgggg cggttcgtgaagacaccacc atttcgctga ttgtgcactt ggagaattgg 360 acaccagaca aaaccttcgatcgcttgggc tctggcgaac agacgcaaat gatctttgac 420 gtgccggttc ccaaaattacgattccggtc aaggtcggtc gtaacttagc cattatcatc 480 gaagtggccg cgatgaatttccgcgccaaa tcgatgggct atgatgccac taaaacattt 540 gaaaagaatt taaatcatctgatcgaacat aacgaagcga acgaccagaa gagttcggag 600 gaaaaa 606 28 1023 DNALactobacillus rhamnosus misc_feature (1)...(1023) n = A,T,C or G 28atgtctatct ccacgcgtgc aaataaactc gacggcgtcg agcaggcatn tgtggcgatg 60gcgaccgaaa tgaataaagg cgtgctgaag aatttaggac tgctgacgcc ggagctggag 120caggcgaaaa acggcgacct gatgattgtc atcaatggta aatcgggtgc ggacaacgag 180cagttactgg tggagattga agaactgttc aacaccaaag cgcaaagcgg ctcgcacgag 240gcgcgttacg ccactattgg cagcgccaaa aagcatatcc cggaaagtaa cctggcggtg 300atttcggtca acggtctgtt tgccgctcgc gaagcgcgtc aggcgctgca aaacgatctc 360aacgtgatgc tgttttccga taacgtctca gttgaagatg aactggcgct caagcaactg 420gcccacgaaa aagggctgct gatgatgggg ccagactgtg gcacggcgat tatcaacggc 480gcggcgctct gttttggtaa cgccgtgcgt cgcggcaaca tcggtattgt tggcgcatcc 540ggcaccggca gtcaggagtt gagcgtccgc attcatgaat ttggcggcgg cgtttcgcaa 600ctgattggca ccggcgggcg cgacctgagc gagaaaatcg gcggcctgat gatgctcgac 660gccatcggga tgctggaaaa cgatccgcaa actgaaatca ttgcgcttat ctccaaaccg 720cctgcgcctg cggtggcccg caaagtgctg gaacgtgcgc gcgcctgccg caagccggtg 780gtcgtctgct tcctcgatcg tggcgaaacg ccagtggatg agcaggggct acagtttgcc 840cgcggcacca aagaggcagc gctaaaagcg gtgatgctct ccggcgtgaa acaggaaaat 900ctcgacctgc atacgcttaa ccagccgttg attgcggatg tgcgtgcgcg tctgcaaccg 960cagcagaaat acattcgtgg cctttctgcg gcggcacgct gtgcgacgaa accatgttcg 1020cgg 1023 29 1227 DNA Lactobacillus rhamnosus 29 cagatcctga ataacccatttttaaataaa gggactgctt ttacgcagga ggaacggaac 60 caatatggct tgaatggtttgctgccacca gctgtacaaa cacttgatca gcaggttaag 120 caagcttatg cccagttgcagaccaagcca actgatttgg ctaagcgtca atttttgatg 180 accttgttca atgagaatcatgttttgttc tataagcttt tctccgagca tatcaacgaa 240 ttcatgccaa ttgtttacgatccgactatt gccgacacga ttgaaaacta cagtgcgctt 300 tttgttaatc cacaaaatgccacgtatctt tcaatcgatg atccggacca tatcgaaagc 360 gcactgaagc atagcgcagatggccgcgat attcggttgc tggttgtaag cgatgctgaa 420 ggcattcttg gcattggcgattggggcaca caaggtgttg acatctcagt cggtaaatta 480 atggtttata cggctgctgccggcattgat ccgagccagg tcttgccagt ggtcttggat 540 gtcgggacta acaatgaaggtttgttgaac gacgaccttt atttaggcaa tcgtcacaag 600 cgcgtatacg gtgaaaagtatcaccacttt gtcgataaat ttgtcgccgc agcagaaaag 660 ctgttcccga acctgtatttgcattttgaa gactttggac gcagtaatgc tgcagatatt 720 ttgaatcaat ataaagacaagatcactact ttcaatgatg acattcaagg caccgggatc 780 attgtcttgg ctgggctattaggcgccatg aatatttcca agcaaaaatt gaccgaccaa 840 gtttatttga gctttggtgccggaactgcc ggtgctggca ttgcttcgcg agtttacgag 900 gcctttgttg aagaaggattgagcccggaa gaagccaaga agcatttcta cttggtggac 960 aaacaaggct tgctctttgatgacatgacg gatttgacgc cagaacaaaa gccgtttgcc 1020 cgttctcgca gcgagtttgctaatgcagac gagctgacaa cgcttgaagc tgtcgttaag 1080 gcagttcatc caacagtcttggttgggacg tcaaccgttc cgggtacgtt tacagagagt 1140 atcgtcaagg aaatggctgcccacaccgat cgaccgatta tcttcccatt gtccaatccg 1200 acgaagctgg ctgaagctaaagcagat 1227 30 1158 DNA Lactobacillus rhamnosus misc_feature(1)...(1158) n = A,T,C or G 30 atgatcaaac ccgaaaagac aatcaatggaaccaaatgga ttgaaacgat tcaaatcaat 60 gccgaagaac gggcaaccct cgaagatcagtatggcgtcg atgaagatat tattgagtac 120 gtcactgata atgatgaaag tactaattatgtttatgata tcaatgagga cgaccaatta 180 ttcatctttc tggcgccgta tgccctcgacaaagatgcgc tgagatacat tacccagcca 240 tttggcatgt tgctccataa gggcgttttattcacgttta atcaaagcca catacctgaa 300 gtcaacacgg cactttactc ggcattggataatcccgagg ttaagagcgt cgatgcattt 360 attctggaaa cactgtttac agttgttgacagctttatcc caatttctcg cggcattacc 420 aagaaacgca actatttgga taaaatgttgaaccggaaga cgaagaacag tgacttggtt 480 tcactttcat atcttcaaca gacgttgacctttttgtcca gcgcggtcca aaccaatctc 540 agtgaactcg atctcaacgg cagtgacgcccttcagcaga ttatcgaatt gctcaatcag 600 catcccctcg actntgcgcc agatgaaaaaggtgcctatt ccaatagtaa ctactatctc 660 ctaggacaca ttattacgca ggttgcgaatatgccgctga gtgattttct caaccaacac 720 ttctttgaac cattggcaat gacgaaaactcaactgggta cgcaacatgc tgatgccaat 780 agttacgatg atttggactt tactaacggcaaaccagtcg cccttggccg cggccactac 840 caaggtggag atggcgcggt ggtgagttcactcgcagact tggccatctg ggcgcgagcc 900 gttttacagc gccgcatttt gccggaatccgcgtgggatg aggcactgac gctgacccac 960 gacttttacg gcatgggttg gatgaaatcccgaacacagc actggttaag tcacaatggt 1020 catattttcg gttactgggc gttttttgatgtttcatttg aaaagcaatt agcacagatt 1080 acgctgacca acatgtcgcc tggtgttgagacactcaaaa aatggcaaga ggagatggct 1140 aactggcgcg catcgtta 1158 31 1071DNA Lactobacillus rhamnosus 31 ttggacaatc aagatgccga ttttaagcccacaatccaaa ttctggatga agttggcaaa 60 gtcgtcaatc ctgatattat gcccgatttgagtgatgatc agctggtcga tttgatgtca 120 aaaatggttt ggcaacgcgt gctggatcaaagggcgacag cgttgaatcg gcagggacga 180 ttaggctttt atgccccaag tgcaggcgaagaggccagca tgatcggtag tcacgctgct 240 atgaagtcat cagattggct gcttccagcttaccgtgatt taccgcaatt gattcaacac 300 gggttaccgc ttgacaaagc ttttctctggtcgcgcggtc acgttgccgg caatgagtat 360 ccggaagatt ttcacgcatt accgccgcaaatcattattg gtgcgcagta tgttcaaact 420 gcgggtgttg cgctcggttt gaagaaaaatggcagtgatg aggtggcctt cacctatacg 480 ggtgatggcg gtacttcaca aggtgacttttatgaaggcg ttaactttgc tgggcatttc 540 aaagcgccgg cactgtttat tgttcaggacaacggctttg ctatttccgt gccgcgggcg 600 agtcaaacgg cagccaaaac gcttgcgcaaaaggcggttg cagccggtgt tcccggcgtg 660 caggttgacg ggatggacgc tttggcagtctatgaagtca ccaaggaagc gcgtgcatgg 720 gcggctgctg gcaatggacc ggttttaatcgagacgttga catatcggta tggcccacac 780 acgctatcag gtgatgaccc aactcgttatcgctccaaag agaccgatga gttatggcaa 840 aaacgagatc cgttaattcg aatgcgcaactatttgaccg ataaaggctt gtggagcaaa 900 gacaaagaag atgccttgat tgaaaaggtcaaagatgaaa ttaaagatgc tatcaataag 960 gccgataaag cgccgcagca gacggtatcgcgcttcttga aagacaccta tgaagttgcc 1020 ccgcaaaatg ttgctgaaca attggcagaatttcaaggaa aggagtcgaa g 1071 32 1310 DNA Lactobacillus rhamnosus 32tctgtactga acatcaatgg cgggaatttg accctgactg atgatggcgt atctgccggt 60actctgactg gaggtggctt cctgaatatc agcggcggcg tcctcgatat tacaggaggc 120aaccacactt ttgctgtcag caccataata gcaaaagatg caactgtccg aatgaacgat 180gtttccggac tgggtactgg taacatcagt aacgctggga cattatctct tactcatgcc 240tcaggcttgc taagtaataa tctgagtggt tctggtacag tatctttgat caatagtgat 300acccagattt caggaaataa cagtaactac tcagggctgt ttgtagtaga taccagctct 360cagttgactg ccactggagc gcagaatctt gggattgctt ccgtgagtaa ccgtggaatc 420ttgcagctga acaacacaac agactggcaa cttataaata atgttactgg aaccggtaat 480gtccgtaaaa ccggttccgg ttcactgact gtccgaagca atgctgcctg gagcggacag 540acagatattg atgacggctc tctgattctt gggcaatctg atgcacctgt catgctcgcc 600agtagcctgg tcaatattgc aaagaacggt aaacttaccg gatttggtgg cgtagtaggg 660aacgtaacca atagtggaag ccttgacctg cgatcggcgg ctccgggaaa tattctgacg 720attggaggta actacaccgg taataatggc acgctgctca ttaacacagt gctggatgat 780agctcttctg caaccgacaa actggtaatt aaaggcgatg cgtccggtaa gacccgagtg 840gctgttacga atgttggtgg ttcaggcgct aatacgctga atagtattga agttattcat 900gttgatggta atgcagctaa cgctgaattt attcaggccg gacgtatagc ggccggcgct 960tatgactaca ctcttggacg tgggccggga agcaactatg gaaactggta tctgagcagt 1020agtaaaaata ctccagaacc aaggcctgat cctgaaccca ctccggaagg gcatgataac 1080aacctgcgcc cggaagccag ctcctatacc gcgaatatag ctgcggcaaa caccatgttt 1140gtgacccgcc ttcatgaacg tctggggcag acgcaatacg tcgatgcaat taccggagaa 1200ccgaaagcaa ccagtatgtg gatgcgccat gaaggaggac ataaccgctg gcgcgacggt 1260tctggtcaac tgaaaactca aagtaatcgt tatgtgattc aactgggtgg 1310 33 645 DNALactobacillus rhamnosus 33 atgaagattt tgattaccgg cgcacaaggc caactaggcaccgaactacg ccacttattg 60 gatgcacgcg gcattactta tcgggcaact gatgccaaagacttagatat taccgatgaa 120 gccgccgtta atcagtactt tgcggactat cagccagacgtggtgtatca ctgtgctgcc 180 tatacagccg ttgataaagc cgaagacgaa gcaaaagcgctcaatcaatt ggtgaacgtt 240 gacggtacgc gtaacttggc taaagcagcg gccaaagttgatgcaacctt ggtttacatc 300 agcaccgatt acgtgtttga tggcgatagt aaggagatttacaccgttga cgatcagccg 360 gcgccacgca atgaatatgg gcgggctaaa tacgaaggcgaacagcaggt gcaaaagtac 420 cttaagaagt actacatcat tcggacttct tgggtctttggtgaatatgg tcacaacttt 480 gtctacacga tgttgaacct cgccaaaacg cataaggaactgaccgtggt ggacgatcat 540 caagaatctt tttccgtctc atcatcacgg acatttgtgaaatatcaaca cgaacacctg 600 atttattccc gacctgtgcc atatcggccc caccttcctggcata 645 34 1920 DNA Lactobacillus rhamnosus misc_feature (1)...(1920)n = A,T,C or G 34 atgcttggag gaaaacagat gcctgaagta aagaaatttg aagccggcacttatgatgtc 60 atcgttgtcg gtgctggtca cgctggnntt gtgaagccgg ccttggctgcagcacgcatg 120 ggcgaaaaga cattattact gacgatcagc ctggaaatgt tggcatttatgccatgtaat 180 cccagcttag gcggtccggc caaaggaatt gtggtccgtg aaattgatgccctcggcggt 240 gaaatgggga agaatattga tcggacctac atccagatgc gcatgctcaacacgggtaaa 300 ggtccggcag tgcgcgcatt acgtgcccaa gcagataaag cggcctatcaccgcagtatg 360 aaacacgtca ttgaagatac gccgcatttg gacttacggc agggactcgctaccgaagtg 420 ctggtcgaag atggtaaggc agtcggcatc gtggctgcta ccggggccatttatcgcgcc 480 aagagtattg tgctaacagc tggcaccagt tcccgcggta aaattattatcggcgaactc 540 atgtatagtt ccggccctaa caatagtctg ccaagcatta agctctctgaaaatctggag 600 cagctgggct ttaagctgcg ccgcttcaaa accggaactc cgccgcgtgttaatggcaat 660 acgattgact tttccaaaac cgaagaacaa ccaggcgata aaacaccgaatcattttagt 720 tttacaacgc cggattcggt ttatctcaaa gatcagttga gttgttggatgacgtacacc 780 aacgcgacga ctcatcagat tattcgggag aatctggatc gcgcgccgatgttttccggc 840 gtgattaagg gagtcgggcc gcgttactgt ccatccattg aagataaaattgttcgcttt 900 gctgataagc cgcggcatca attattcttg gaacctgaag gccgcgacacctcggaatat 960 tatgtggggg acttttcaac gtccatgcct gaggaaatcc agttgaaaatgctgcacagt 1020 gtcgcgggat tggaacatgc cgaactcatg cgcgccggtt atgccatcgagtatgacgtc 1080 atcgagccat ggcagttgaa agcaacgttg gaaactaagg ttgtggagaatttgtatacc 1140 gccggacaaa tgaacggtac cagtggttat gaagaagctg ccggtcagggaattgtggcc 1200 gggattaatg ctgcccgccg cgctcaaggc aaaggaccct tcacgttgaagcgttcagat 1260 gcttatattg gcgtgatgat agatgatctc gtgacaaaag gaacgaatgaaccgtatcgt 1320 ttgttaacca gccgcgccga gtatcggttg ttactgcgtc atgacaatgcggatctgcgc 1380 ttaacgccaa tgggacatga acttggcctg attagtgatc aacgctatgctgtctttttg 1440 gctaagcgtc aagccattac cgatgaatta gcgcgccttg agcacacccgcctgaagccc 1500 aaggatgtca acccgtggct tgaagctcat cattttgcct cccttaaagatggggtctta 1560 gccagtgact tcttgaagcg tccggaaatc aattatcaga cgctagaacagttcttaccg 1620 gaaaacccaa ccttggatca tcgggtgatt gaacaggttg agatccaaatcaaatacgcc 1680 ggctacattg ccaaagaaga agnccagtgt gccaagttga agcggcttgaaggcaaaaag 1740 attccggcac gtatcaacta cgaagcaatc aatggcttag caaccgaggcacggcaaaag 1800 ctggttaaga ttcaaccgga aaccattgcc caagcaagcc gaatcagtggcgttaatccg 1860 gctgatgtcg ctattttgtc cgtgtatatt gaacagggac gaattagtaaggtggcacag 1920 35 762 DNA Lactobacillus rhamnosus 35 cccctgtcaaccatgatgtt ggctgggatt cgcgatatct tggtcatttc aacgccgaga 60 gatattgatcgttttcagga tctgttaaaa gacggtaaac aactgggact caatattagt 120 tacaaaatacaggaaaagcc aaatggcctg gcggaagctt tcattgtcgg ggctgacttc 180 attggcgatgattctgtgtg cttgattctc ggcgacaata tcttttatgg cagcggcttg 240 tccaagctggtgcagcgctc ggcggctaaa acaaccgggg caacggtgtt cggctatcaa 300 gtcaatgaccctgagcgttt tggcgtagta gcctttgacg agcagcatca tgtgcaatcg 360 attgtcgaaaagccagagca tccggagagt aactttgcgg ttaccggcat gtatttctat 420 gacaaccaagtggtggacat tgctaagaac cttaaaccgt caccacgagg cgaactagag 480 attacggatgtgaacaaagc gtatctcgaa cgtggccaac ttgatgttga gctgttgggt 540 cgaggatttgcttggttaga tactggcacc catgaatcct tacatgaggc ggctagtttt 600 attgagaccgttcagaagcg gcagaatctt aaaattgcct gtcttgaaga agtagcctac 660 cggatgggttacattgatcg cgatcaatta cgcaaactgg cgcagccgct taagaagaat 720 gattacggtcagtacatttt gcgcttagca gacgaagaag ac 762 36 936 DNA Lactobacillusrhamnosus 36 atggcaatta acctagttgg gattaatgac gcgaatttaa cgttaattgaagaaggcctg 60 aacgtccgga tttcgccgtt tggggacgaa ttacgcatca gcggcgaaaccgaagcggtc 120 agcttgacac tacagctgct tgaggcggca actaagttat tagcacaaggcatcaaactg 180 tcacctcagg atattgctag tgcggttgca atggcaaaac ggggtacactcgaatatttt 240 gcggatatgt atagtgagac cttgttacgc gacgccaagg ggcaaccgattcggattaag 300 aattttggcc agcgtcaata tgttgatgcc atcaaacaca acgatattacttttggcatt 360 ggcccagccg gtaccgggaa aacttttctc gccgtggtga tggcagttgcagccatgaag 420 gccggccaag tcgagcggat tattttgacg cgtccggccg tggaagcaggcgaaagtctt 480 ggctttctcc ctggtgatct caaggaaaag gttgatcctt atttgcgtccggtttatgac 540 gctttatatg ccgttttggg gaaagaacac accgatcgcc taatggatcgcggcgtcatc 600 gaaattgcgc cattagcgta tatgcgtggt cgtacgttgg acaatgcgtttgcgattttg 660 gatgaagccc agaatacgac tcaggcccag atgaaaatgt ttctgacgcgcctgggcttt 720 ggctcgaaaa tgattgtcaa tggtgatgtg acgcaaattg acttgccgcataatgccaaa 780 agtggcttat tgcaagcgga acagttatta aaagggatta gtcatattgccttcacgcaa 840 ttttccgcac aggatgttgt gcgccaccca gttgtcgcca agattatcgaagcttatggc 900 aaacatgatt tacagctgca aaagcaaacg aaggag 936 37 840 DNALactobacillus rhamnosus 37 atgaagaagt tcgacaagat gatggactgg ttagcagatgtctatgtcaa tgccctgaat 60 gttattcact acatgcacga caagtactac tatgaagctgcgcagttggc attgaaggat 120 acacgtctga atcggacgtt tgctaccggg atttccggtttgtcacatgc ggttgattcc 180 atcagtgcca tcaagtatgg ccacgtgaag gcaattcgggatgaaaatgg cgttgcaatc 240 gactttgttg ctgacaatga tgactatccg cgttatggtaacaatgatga ccgggcagac 300 aacattgcta agtggttggt taagaccttc tacaacaagatgaatacaca tcatctgtat 360 cgcggtgcta agctcagtac cagtgttctg accattacatcaaatgtggt ttatggtaag 420 aataccggga caacaccaaa cggccgtcag aagggcgaaccattctcacc tggagccaac 480 ccagcgtatg gcgctgaaaa gaacggtgct ttagcttccttgatgtcaac cgccaagatc 540 ccatatcact atgcaacaga cgggatcagc aatacctttggggtaacacc gaacacctta 600 ggccatgacg atgaaactcg taaggacacc ttggttcacatggttgacgg ctacatggaa 660 aatagcggca tgcaccttaa catcaacgtc ttcaataaagaaacgttgat tgatgcccag 720 aagcacccag aagaatatcc aactttgact gttcgagtttccggttactg cgtctacttt 780 gcagacctga ccaaagaaca gcaagacgat gttatcgctcggaccttctt cgacgaaatg 840 38 1341 DNA Lactobacillus rhamnosus 38atggcatttt caaaagaaac ccgcacccag acgattgatc aattgaagca gaccgaactc 60gacttactca ttgttggcgg tggtattacc ggtgccggcg tagcaattca ggcagcagca 120agcggtttaa aaaccggctt gattgaaatg caggatttcg cggaaggaac cagttcccgc 180tcgaccaaat tggttcatgg cggcattcgt tacctcaaga cgtttgatgt cggcgttgtc 240gccgataccg ttaaagaacg tgccgtagtt caaggaattg cgcctcacat tccgcgacca 300ttcccgatgt tgttgccgat ttatcaggaa gccggcagta cttttgacat gttcagtatc 360aaaatcgcca tggatctcta tgatcgtctg gcaaacgttg aaggttccca atacgccaac 420tacaccgtca ccaaagatga aattctgcaa cgtgaaccgc atttagcctc tgatggcctc 480caaggcggcg gcgtgtacct cgattttgtc aacaacgatg cccggcttgt tattgaaaac 540atcaaagaag cagcagaatt aggcggactg atggctagtc gggttcaagc cattggcgtt 600ttgcatgatg atgcaggtca ggttaatggc ttacaggtta aggatctttt ggatggcagc 660gtttttgaca ttcatgccaa actcgtgatc aatacgaccg gaccttggtc tgacaagttc 720aaggcgttgg atcaagccga agatcaaacg ccaacattgc ggccaacgaa aggggttcac 780ttggttgtcg atggttctcg actgccggta ccacagccaa cgtatatgga tactggcttg 840aacgacggcc ggatgttctt cgtggtgcca cgggaaggca agacttactt cggcaccacc 900gataccgatt accatggcga tttcaaccat ccgcaagtcg aacaagccga tgtcgattat 960ctcttgaaag tcatcaacaa gcgctatccg caaagccata tcacgcttga cgatatcgaa 1020gcgagctggg caggattgcg accgctgatt gccaacaacg gcagctccga ttacaacggc 1080ggcggtgcga acaccggtaa agtttcggat gattcctttg aagcgttaat ccgtgtcgtt 1140gatgattacg aagacaacca ggctacccgc gctgacgttg aacatgcgat ctccaaacta 1200gaaacagccc acgccgaagc tgctttgagc ccatcacagg tttcccgcgg cagctcactt 1260cgccaagccg atgatggcat gatcaccttg tccggcggga aaatcacgga ttatcggaaa 1320atggcagcgg gcgcgcttgc t 1341 39 726 DNA Lactobacillus rhamnosus 39gatctcttct gcccagatat cacggcagat attctgactc gtaaagacga tcttggtagt 60gacaagccga tcgttgatgt gattttggac cgcgctggca acaaagggac cggcaaatgg 120tcttcacaat ctgctcttga gctaggtgtt ccgcaaagtg tgattaccga atccgtctat 180gcgcgttaca ttagtgcgat gaagcaggag cgggttgcgg caagtaaagt tctgccaaag 240ccggtcggaa atgtcacgat tgacaaaaaa gaagctatcg agatgattcg taaggcgtta 300tacttcagca agctgatgtc ctatgctcaa ggctttgaac aaatgcgcgt tgcatcggat 360aactacgact ggaacctgca gtacggtgaa ttggccaaga tttggcgtgc aggttgcatc 420attcgcgcac gtttcttgca aaatatcacc gatgcctacg ataagaagcc agatttacag 480aacttgttgt tagacgatta cttcctgaat attgctaaga actatcagga aagtgttcgt 540gacttggtcg gcttggcagt taaagccggt gttccggtgc cgggcttctc agcggcgatc 600agttactacg actcttatcg cgcccctgtt ctgccggcca acctgactca ggctcaacgc 660gactactttg gtgcccacac atatgaacgt actgatcgtg atggcatttt ccattacacc 720tggtac 726 40 972 DNA Lactobacillus rhamnosus 40 gaagatttct ttatacagataagcgctacc cagcatcact gaatccccga ctgctgtgat 60 cagattccga ccggtgacttttcattcttc gataacactt tggacgttgc aaatctttta 120 aacattgtac ccaagcgctaccaagacctg aacttatcac cgcttgacac ctactttgcc 180 caagcgcgtg gctatcaaggagaggccggc gatgttaaag ctctggcgat gaaaaagtgg 240 ttcaacacca actatcattacttggtacct gaattcgatc gcgataccaa gatccaagta 300 acggattggc agcttttcgtgcaatttgaa gaagctaaag cgctaggcat taacggacgt 360 ccgactttga ttggaccgtatacgttactg aaattgtctc gcttcattga tgttgtgcct 420 gatgactttg tagccgacctgatttctgcg tacacgacca tcattgatcg cttgcatgac 480 gccggagcag actgggtacaacttgacgaa ccggcgctgg tttatgatca aaccgatgcc 540 gacctcgcct tattcgagcggctttatacc ccgattttga cccaaaaaaa agctgccaaa 600 atcctggttc agacttattttggtgattta accgattcgt ttgaccgtat tcaaaagttg 660 ccatttgacg gcttcgggctggattttgtc gaaggatatg ccaatcttga tctgctcaaa 720 caacacggct tcccagcgcacgctacctta tttgccggaa tcgtgaatgg taagaacatt 780 tggcggacac attatgccgatgccttggca acgatcaaac aactggcaac cattacggac 840 aagctggtat taagcacctcgacctcactc ttgcatgtcc catatacact tcgcaatgaa 900 acccatctga aacctgaagaaaagcaatat ttggcctttg ccgaagaaaa actcaacgaa 960 ttgcatgagt ta 972 41 888DNA Lactobacillus rhamnosus 41 gggccggcga ttttcggctt tattccgatgcaggatggct cgcccgcgcc ggggctgagt 60 aatatcacgg cagaaggctg gttcccgcacggtggcttac cgattttgat gactatggtg 120 gcagtgaact ttgctttttc gggtaccgagcttatcggca ttgccgccgg tgaaacggaa 180 aacccgcgca aagttatccc ggtagcgattcgtactacca tcgcgcgact gattattttc 240 tttatcggca ccgtgtttgt gctggcagcgctgatcccga tgcagcaggt gggcgtggag 300 aaaagcccgt ttgtgctggt atttgagaaagtagggatcc cgtacgccgc tgatattttt 360 aacttcgtga tcctgacggc tattctttgtgcagcgaact ccgggttata tgcctccggg 420 cgcatgctgt ggtcgttgtc gaatgaacgtacgctaccgg cctgttttgc gcgagtaacg 480 aaaaacggcg tgccactgac ggcgctgtcggtcagtatgc tcggtggtgt gctggcgctg 540 ttttccagcg tggtggcccc gaacacggtatttgttgcgc tgtcggcaat ctccgggttt 600 gcggtggtag cggtgtggct gagtatctgcgcctcgcatt ttgtttttcg tcgccgtcat 660 ctgcaacaag gtaaggcatt gagtgaattacattatcgcg cgccgtggta tccgctggtg 720 ccagtattag gttttgtgct gtgcctggtggcctgtgttg ggctggcatt cgatccagcg 780 cagagaattg cgttgtggtg cgggttaccgtttgttgcgt tgtgctatgg tgcttatttc 840 cttactcaac cccgaaacgc aaaacaggagccagaacatg tcgcagaa 888 42 1422 DNA Lactobacillus rhamnosus 42atgcgtaaac aattacccaa ggactttgta atcggtggcg caactgctgc ttaccaagtt 60gaaggggcaa ccaaagaaga cggaaaaggt cgagttcttt gggatgattt tctggaaaaa 120caagggcggt ttagtcctga ccccgccgct gatttttatc atcgctatga tgaggatttg 180gcgttagcag aagcatatgg tcatcaagta atacggcttt caattgcctg gtcgcgaatt 240ttttcggatg gtgccggggc ggtggaatct cgtggcgttg ctttctatca tcggctcttt 300gctgcctgtg ccaagcatca tcttatcccg tttgtaacgt tgcatcattt tgatacacca 360gaacggttac acgagattgg tgactggctg agtcaagaaa tgctggaaga ttttgtcgag 420tacgcgcggt tttgcttcga agaatttccg gaaatcaaac actggattac gatcaatgaa 480ccaacgtcca tggcagtgca acaatatacg agcggtactt ttccaccagc ggaaaccggt 540cattttgata aaacatttca agccgaacat aatcaaatcg ttgcccatgc gcgtattgtt 600aatttgtaca agtcaatggg gctagacggt gaaatcggta tcgtgcatgc cttgcagaca 660ccttatccat atagtgattc gtcggaagat cagcatgccg ctgatttaca ggatgcgttg 720gaaaatcggc tgtatttaga tggcacactg gcaggagatt atgcccctaa gaccttggct 780ttgatcaaag aaattctggc agccaatcaa caaccgatgt ttaagtacac tgatgaagag 840atggcggcta ttaagaaggc ggcgcaccag cttgattttg ttggcgttaa taattacttc 900agcaaatggc tgcgcgctta tcacggcaag tcggaaacga ttcataatgg tgatggctca 960aagggatcgt cagttgcccg ccttcacggt atcggcgagg agaagaagcc agccgggatt 1020gagacaacgg attgggactg gtccatctat ccgcgtggta tgtatgacat gttgatgcgg 1080attcaccaag attatccgtt agtaccagcc atctatgtca ccgaaaacgg tattggattg 1140aaagaatcct taccagcaga agtgacgcca aatacggtca tcgcggatcc caaacgcatt 1200gattatttga aaaaatattt aagtgccatt gccgatgcga ttcaggctgg cgcgaatgta 1260aaaggctact ttgtctggtc actgcaggat cagttttcct ggacaaatgg ttatagcaaa 1320cggtacggat tgtttttcgt cgactttccg acgcaaaaac gttatgtcaa gcaaagtgcc 1380gaatggttaa aacaggttag ccaaacgcat gtgattcccg aa 1422 43 774 DNALactobacillus rhamnosus 43 atgacgacct tgaaatcatt ccgggtgatc aataaggttgacttgcctag tgcccagcct 60 gatgttgtta aagaagagat tgaagagatg atcggcttagacgcttctga cgccattttg 120 gccagtggta aaaccggctt gggagttcct gaaattcttgagcgcatcgt ctcagacatt 180 ccggctcctt ctggcgatgt taacgcgccg ttgcaagcgttgatctttga ttccgtttat 240 gatgattatc gcggtgttgt ccttgatgtt cgggttaaagaaggacaagt taaggtcggc 300 gatacgatcc agctgatgag caatggcaag cagtttcaggttactgaagt cggcgtgatg 360 tcacctaaag cagtgaaacg cgattttcta atggtcggggatgtcggtta tatcacggcc 420 tcaattaaga cgattcagga tacgcgcgtg ggggacacggtgacgctggc agaccgcccg 480 gcagcagccc cgctgaaagg gtatcgtaaa atcacgccaatggtctattc aggcctgttt 540 ccagtagata acgcgaaatt caatgacttg cgcgaggcattggaaaagtt gcaactcaac 600 gatgctgcgc ttgaatttga gccggaaacg tcccaggcactcgggtttgg gttccgttgt 660 gggttcttgg gcctcttgca tatggatgtg gttcaggaacggctggaacg cgattacggt 720 ctcgatttga tcatgaccgc accgagcgtg gactatcaggtagcgttaac agat 774 44 1254 DNA Lactobacillus rhamnosus 44 atggatgtcaccacgattga tctggaacaa atgggccgag cagcaaaggc cgcggcgact 60 gtcttgagccagttgacgac cgcacagaaa aatgccgggt tgttggccat ggttacggct 120 cttgaaacgcatactgaaac aattttggga gctaatcatg aagatctaaa agcggcagca 180 agcttgccggctaagttcac ggatcgattg gtactgacag ccgagcggat tgctgacatg 240 gcagcaggggttcgccaagt tgccgcctta cctgatccaa ccgcccagac ggataaggcc 300 tgggtgaatcacgcaggact gaatattgcg caaaaacggg tacctttagg ggtggtcggg 360 atgatttatgaggcccggcc aaatgtaacc gttgatgctg ctgcgttaac ttttaaaagt 420 ggcaatgcggtcattctccg tggcggtaaa gaggcgctgc acagcaattt ggccttggcg 480 accgttttacaggctgcatt gaccgcacaa ggattgccaa aagacgcgat tcaattaatc 540 acggacccgaagcgagaagt cgcgaatcag atgatgcacc tgaatggcta cattgatgtg 600 ctgattccgcgtggtggccg agggttgatt aaagcagtcg ttgaacaggc taccgtaccg 660 gtcattgaaaccggggcggg caattgtcac atttatgttg atgcgtatgc gcaagcccag 720 atggcaatcgacattgttgt caacgccaaa gttcagcggc cgtctgtttg caatgcggcc 780 gagaaacttttaatccacgc tgatgttgca aacgcgcagc tgcctttaat tgctgcggca 840 ctgcaagcgcatggtgtcga attgcgcggt gatgaacggg cgcgggcaat tgtgccgaac 900 atgcagatcgccacggaaga agactgggat accgaatata atgacttaat tatggcggtc 960 aaggtggtggattccgagga agaagcgatt gcgcatatca acgcacacaa cacgaagcac 1020 agcgaggccatcattacgga taactaccaa aatagtcagc aattcctcca acaggtagat 1080 gcggctgttgtctatgtgaa tgcctcaact cggtttacag acggcttcga gttcggtttc 1140 ggcgcagagatcggtattag tacgcaaaaa ttacacgcac gcggaccgat ggggttagcg 1200 gcgttgacgacgattaagta tcaggtgctg ggtaacggac aggtacgcga aggt 1254 45 489 DNALactobacillus rhamnosus 45 atgaccgcat ttttatgggc acaggatcgc gatggcttaattggcaaaga tggtcatttg 60 ccatggcatt taccggatga tttacattat ttccgggcgcagacagttgg taagatcatg 120 gtcgttggcc ggcgcaccta tgaaagtttt cctaaacgtcctttacctga gcgaaccaat 180 gttgttttga cccatcagga agactatcaa gcgccaggtgccgtggtcgt gcatgatgtt 240 gcggcggttt ttgcttatgc taagcagcat cccgatcaggaactggtcat tgctggcggt 300 gcgcaggtct ttacagcgtt taaagatgat gtcgatacgttattggtgac acggttggct 360 ggcagttttg aaggcgacac gaaaatgatt ccattaaactgggatgattt taccaaagtc 420 tccagccgca ccgttgaaga taccaatccg gcgctgacgcacacttatga ggtttggcaa 480 aagaaggct 489 46 285 DNA Lactobacillusrhamnosus 46 caagggtgca acctttacgg tatcgcaact gctttggcac ggatttccaaagcaattctt 60 aacgatgaaa atgcggtact cccattgtcc gtttacatgg acggccaatatggcttgaac 120 gacatctaca ttggtacacc tgctgtgatc aaccgcaatg gtattcagaacattctggaa 180 atcccattga ccgaccacga agaagaatcc atgcagaagt cggcttcacaattgaagaaa 240 gttctgaccg atgcgtttgc taagaacgac atcgaaacac gtcag 285 47969 DNA Lactobacillus rhamnosus 47 atgttgacga aacggcaatt gctcgtcttaaaagaaatca tccgcctgtt tactgaaagt 60 gggcagccgg tcggttccaa gacgttaatgcaggaactgc cggttcatgt cagttccgcc 120 acgatccgca atgatatggc atcgctggaagacgccggtt tgatcaccaa gactcatagc 180 agttccggtc gagtaccctc gactcaaggctatcgctact atcttgatca tctggtcgag 240 ccagtgcgtg tttcccaccg tgaactagccacgatcaagc aagcattcgg tcaacgctac 300 aataagatgg atgaaattgt ggcgcaaagtgcgcagattt tatccaatct gaccagttac 360 acggcgatca gcttagggcc agaagtgaataacattaaat tgaccggatt tcgccttgta 420 ccgttgggca atcaccaggt tatggcgattttagtgacga acaacggcaa tgttgaaaat 480 caggttttca ccgttcctga aagcatctcatctgacgagc tggaaaaggc gattcgcatt 540 gtcaatgatc agctggtcgg tttgccgcttattcaagttg ctcagcggct aaagactgat 600 gttccgtcga tgctgatgca gtatctgaccagtcctgaag gcttcctgga tatcttcggt 660 aatgtcttaa agtccgccgc ttcagagcgtttctatgtgg gtggccgctt aaatttaatg 720 gactatctcg gcgactcgga tattcacgagttgaaaaaga ttatgtcctt gattgatgct 780 gatcatggtg atcttactga actgcttggcggaccggttc gccaaacgcc ggttacggtt 840 cgtctaggcc cggagttaaa gccaattgatctggccaatc tcaagctgat taccgccagt 900 tatgatgtcg gtgaccacgg cacgggaatgattgccctat tagggccaac ccaaatgccg 960 ttttccaag 969 48 1336 DNALactobacillus rhamnosus 48 ctaattcacc gtatacagcg gcgaataaac tgaagatgacggtggctaag acgctggaca 60 agtttcagct ggatgtgacc acaggcaacg ggcttaaggcgattaacatt ttcatgaatg 120 acagcacgaa ggaaaatgtt gaacaatatg aatattggattaacaatttc attgaacgcg 180 gcgtgcttga gccaaaataa gtctctttga aaatcacggtaactctcctt tcgaacaagc 240 ggagattacc gtgctttttt gttgtatgaa tcacggattaaagcgttacg actggtaaag 300 catgagctac atcacggatg cgtggtttaa gtgaggcaaatgtcgtgacc gccaaatcga 360 gcggccaaaa gtggtaagct ggaaacattg aaattttgatatgaaggagg gcttgtatgt 420 ttgaacatgg gtttattgag gtacacgatg ccaaccagaacaacttgcaa catgtgaatg 480 tgaagatacc taaggatgcc attacggttt ttgtgggtcggtctgggtca ggcaaatcat 540 cgttagtgtt cgatacgatt gctgcggagt cacggcgggagttgaacgaa acttttccga 600 gctttaccca gcaatattta ccaaagtatg gccagcctgatgtcggctcg attgatcact 660 tgccggttgc cattgtggtg gagcaaaaac gtatcgggaaaaacgctcgc tcaactttag 720 caacttacac gggcatttac tcactgttac ggctgttgttttcgcgtgcc ggcaagccat 780 tcatcggcta ttcggacaca ttttcgttta atttacctcaagggatgtgc cccacctgcc 840 aaggcttagg ttacgtagat gatattgacg tcagtaagttgattgatccc aataaatcgc 900 ttaaccaaga ggcgatcacc tttgtcagtt ttggaccggatacttggcgt tggcggcgtt 960 atgcttacag cgggttgttt gataatgaca aacccttacgtgactatacg cccgaagaaa 1020 tgaaactgtt actttatgca ccgcaacaga cactgaagcatgcaccggct aaatggccaa 1080 gaacagcgct atatgaaggt gtcgtgcctc gcattaaacgatccattatt ggtaaaaaag 1140 aagcagaaca tcataaggcc gcactggcag aaatcgtaacgcgcaagcct tgtccggatt 1200 gccaagggac acgcctacgt ccggaagtgt taacctgtttgattaatcaa accaatattg 1260 cccaagtgct gcaaatggac ttggtaaacg tacggcattttctaaaaaac attcaagtgc 1320 cgctggttca ggatgt 1336 49 760 DNALactobacillus rhamnosus 49 aatgacacaa tcggccgatc cccacgcgcc gccattgcttgcaaagtggc ggcaatggga 60 tactgaccgc cataagtcat cgccatttga acatcccaatccggaagtgc ctggcgcaag 120 cgatcgttta ttaactgaag agattgcggg cattttcattttaggaacaa atggcgagtc 180 ttacgtctta gcggaagatg aaaagttggc atttgtagaacatgttatcg actatgtcca 240 tggtcgaacc aaagtactgg taggaacagg tttgaacggaacagcggaaa ccattcgctt 300 cagtcaaaag gtagcgtctt taaaaccaga cgcaattaccttagttgctc cttcatttgt 360 tgctccctcg caacaggaac ttgttgatca cgttgctgccataattcacg cggacgatat 420 acctgttcta ctgtacaata tgccagcaaa aacgggcattaacattgagc cagcttcatt 480 aaaacagttg tcaaaatatg agaacttaat cggtataaaagatagctcgg gaaagtggga 540 gaattttgac ggttatctag ccaatcgccc cgaacgaccattctcagtta ttatgggctc 600 tgacggtcgc attcttgaaa gctttcagca cggtggtaatgcggctattg caagtacagc 660 caatctcctg acggctaaca atgtagcgtt gtatcaagcatttgttaatg acaatattga 720 aaaagcccag aaatttcagg atcggattca gccccttaga760 50 1056 DNA Lactobacillus rhamnosus 50 aagacagttg ccaaaaccccaatcacgaga ctgacaagaa cggctaatac agcagccagc 60 gtacccgtag ttggccaataaggcgcaatc cccgtttcaa tcagaatgac caccgccgcg 120 gcgacaatgc cacaaaccgcgtaataaacc tcaacctcaa aatgttttag taaataaccc 180 attagtttgg caatggcaatgagcccgatc aacgcaccta ccataaaagg taccagtaag 240 gcgacagatc aaggtgtcgactggagccgg taccaaggag ataacggtgt ctttggttac 300 tccactgaca agttcggcatctctcaaatc ggtggctata gcggctacgg cacgtacgag 360 caaaccacgt ataagacacaggttgcatcg ttgattgccg ctggcaagcg agcacacacc 420 tatatctggt ggcagaatatcgacaacacc aattttgcca agcaagtact agatcatttc 480 ttgccagaga ttcaaacaccaaaagggtcg attgttgcgc ttgattacga ggccggttca 540 acaaatacgg caactttgctgtgggcactc gactatatcc gtgatgctgg ttacacgcca 600 atgctgtacg gctataagagcttcttgatg agtcacattg acttgtcaca gattgccagt 660 cgctaccagc tatggcttgcggaatatcct gattacaatg tcactactgt tccgaattat 720 ggctacttcc cgagttttgataatgtaggt atcttccagt tcacttccac ctatcgcgct 780 ggcggccttg atggcaacgttgatcggtct ccgcgaacgc tcacaaaacc tcgctgttgt 840 caatgaagct atcgagaacacagtttcccg tccagcttac gtaacctttg acgacaataa 900 gaaggaaggt tcccttgttcgcttaccaga acgtggcgaa ctcgaaccag aagttgacga 960 atcactggtt gttgaatactataaccagaa actttaatac ttcacagcta cagccaactc 1020 tttgcggagt tggctgtttttatttggaat cagatc 1056 51 1310 DNA Lactobacillus rhamnosus 51 cgccggtgaacgcgtttcag accagagcgt gaacaggagc gcttagaaat cggagtgtaa 60 gtggcctcagacgtgatgac ccgggctttg gccattgcgt tccaggtctt tacacgcaga 120 cccatgcgccggtgaacgcg ttttagaacg tgaaccggcg cgcacacgta acccccccta 180 aaaaaccaccaccccaaacc taacgctcct tagattcaaa cctccaaaca cctcacttgg 240 cactccccatttacagcatt agaatccgcc ctgtttttaa ggtatactaa ggcgtactag 300 accaaaatttaaggtgggac aagcacttga aaaagttcgt caatcgggtc aagacgcttg 360 ggactcgattttgtcgctgg ttcacgcaat tcgtgacccg gcatcccgat tccaaatcag 420 ataccaatcaacaactaacg ggtaaagctg ccgttgtcta ttatggcaac gtcacacttc 480 aaagcatcaaaacaactgtg ttactattta ttaggcgttt taggcattgc tgtcgtgttt 540 ggccttggcttgtttggcgg ctattttgtg tcgattattg acgcaacgcc aattccaact 600 gaaaccgctatgaaagcaac gttgtccaat accagccgca cttccagcat gtattttgcc 660 cacaacgtcaagcttagcga tgttaaaagc gacctgtact caaccaaagt caacctcaat 720 gagatgtcgccatggctcac caaggcgatt atcgccactg aagatgaaga cttctatcgc 780 cacaacggcatcgttcctaa ggcggttatc cgtgcgttct tctccgactt aaccggtatg 840 gggagtcagacgggggggtc aactttaacg cagcaagtgg tcaaaatgat gtttttaaat 900 tcggagacaacctttaaacg taaggctgct gaaattatgc tggcccggcg cttgaacaat 960 cacttcagtaaaaacaccat tctggcaact tatctcaatg ttgctacgct tggtcgtaat 1020 aacaaaggccaaaatattgc cggagttgaa gcagcggctc aaggactgtt tggggtttcg 1080 gctaaggaagttaacctgcc ggaagcggct ttcatcgctg gcctgccgca aagtccattt 1140 gtctataccccttacacggc cgatggtaaa ttgaaaacca gtctcaaagc cggtatcaat 1200 cgccaacaaaccgtcctgtt ccgcatgtat cgggctggcg ttatcagcca tcgtcaatat 1260 gttgctgccaaatcatttga tccactagtg tcgacctgca ggcgcgcgag 1310 52 1859 DNALactobacillus rhamnosus 52 ccttgcagcg atccggttat ggggtttatg ttccgcttttttccgggcat gggacagtgg 60 agccgttaga tattttgaca aaaggcaacc cggatatttggtgggcagaa agtagtgccg 120 cggttgcgca tatgaccgca aaatacgcca aggtgtttgtttttggctta tcactgggag 180 gtatttttgc gatgaaggcg ctagaaacct tgccagggattacagcaggc ggtgtttttt 240 catccccgat tttgccgggc aaacatcact tagtaccgggttttttaaag tatgccgagt 300 atatgaatcg gttagcaggc aaatcagatg aaagcacacagattctggca tatttgccgg 360 gacagttggc cgcaatcgat cagtttgcca cgacggttgctgctgattta aatttagtca 420 aacagccgac ttttattgga caagccggtc aggatgaattagttgatggt cgattagcgt 480 atcaattacg cgatgcctta atcaatgctg cacgcgttgattttcattgg tatgatgatg 540 ccaagcatgt cattaccgtt aactcggccc atcacgcattagaagaagac gtaatcgcat 600 ttatgcaaca agaaaacgag ggatagcatg accacagttggccatattcg taatgaacta 660 ttagcaacat ttcgtaagaa tccgaacatt gattattcggttcaaacact cagtcgcgca 720 cttaagttaa gtgaaggcgg cgattttaaa gtactcgtccaggcgttaaa cggtatggaa 780 aatgataacc tgattcacgc caatcacgaa ggacgttatgcattgggcgg cgcgcctaaa 840 gtcttgaccg gcactttccg cggcaacgaa aaaggctttggcttcgtggc agttgagggc 900 ttggacaatg atgtttatgt accggcgatg aacaccgattttgcgcttga tggcgatacc 960 gttgaagtgc ggatcgttcg tgaagcccgt cccaatgatagtcgcggacc tgaaggcgaa 1020 atcactaaga ttgtgcagcg cagtttaacc acactggttggtgaattcaa accattttcc 1080 gataaagatc gggctaagtc tggatttatc ggaatggtggttagtcatga aaagaaactg 1140 aagaattttc cggtttatgt taaagatacc ggtaatattccgcaactcgg cgatatgacc 1200 gtgacggaga ttactgaatt tccaaccgaa tatcatcccaagttgatgta tgggatcgtt 1260 gtcgagacgt taggcaacaa gaatgatcct ggcgttgatattatgtcgct ggtcatgcaa 1320 aaccatatca aaacggaatt tccggatgaa gtgatggatcagaccaatgc cattcccgat 1380 cacgttacgc cagaagaacg agttggtcgc aaagatattaccgatcaggc tgttgtcacg 1440 attgacgggg atgacagtaa agactttgac gatgccgtggtggtttggaa attaccaaat 1500 ggaaacttcc atcttggggt tcacattgcc gatgtttcgcattatgtgac ggaaggctct 1560 gcgttggatc aagaagcatt tgatcgtggt accagtacgtacttggttga tcgcgtcatt 1620 caatgctgcc atttcgacta tccaatggca tttgttcgttaaatccaggt gtagatcgat 1680 tggcaatgtc atgtgatatg gaaatcgatc atgatggtcatgtcgtaaac cacgagattt 1740 atcaaagtgt gatcaagagt catgcccgga tgacctacaacaatgtgaat aaaatcgtga 1800 ccgatcacga cccggaagtc atggccgaat atcaagaactggtacccatg tttgaagac 1859 53 887 DNA Lactobacillus rhamnosus 53cttatgaaaa tgaaattatc atcacgattc gagctggacg caagaaccat cccttgttgc 60tctcagctaa tccacagtat gcgcgggtgc aaattaccca cattccattt acaaatccag 120acgttcctgc aaccttcacg atgacgttgc ggaagtattt taacgcggct acgttaacag 180agattcacca agtgcaaaac gatcgggtac tacactttga attctccacg cgggatgaat 240tgggggatga actggggctg cgcttgatca ttgaaatgat gggtcggcac agtaacatct 300ttttagtcag caagcgcacc ggcaaaatta ttgatctcat tcgccacgtt tctgcggatc 360aaaatcgcta tcgtccgttg atgcccggtg ccccgtatgt cgagccgcct aagcaagata 420aagtggatcc gtttcatgat tcggagcgga tttatcacga acttgaacgt caggtaacac 480cttcattgag tcgcgccgcc ttgctccagc aacattacca aggacttgcc aaggattctg 540cagctgaatt ggccctgcga ctcaatcaag gcgatgccgg ctgggatagc ttttttgcag 600cgctggcaac ccctgaaccg actattacaa cccaagggaa aaaagccgtt tttaccgcga 660tcccgtatca gtctctgacc ggcgagcagc aacattttcc aaccttaagc gcgatgctgg 720atgcctatta tgcgcaaaaa gcggaacatg atcgggtttt gcaacaaggc gggaacctga 780ttcatgtgat caaaaatgtg attgataaag atcgcaaaaa gcagcgcaaa ttaaagcgaa 840cgctggaaga aaccgaaaaa gccgatgatt atcgaattcg attcaag 887 54 999 DNALactobacillus rhamnosus 54 gcctatttgg gattgacaga aaaacaaacc ggcagcctgtatcacctgag cgaaggccag 60 aaaaaaatgg tgcagctgat cgcaatgtta agtctggagcgaactttttt gctgttggat 120 gaaccgttta gcggcttaga tgaacgtgcc tgtgcattctttgccgcgtg gatcaaggaa 180 aaagcagcta agcaggcgtt tttgattgtg acgcaccgactggctccgct agctggcatc 240 agtcaccagc atgtggcact tgccgatcac cggttacacattctacagga gtgacatgat 300 gccagtaaaa aagaccaatg cagtgaatct gagtttatttattctactgc tgacacttga 360 aatatctttc agccatgcgg tgagtcttaa tgtagcgttgatcggactgg caagcggttt 420 tttaatatgg cggcgggcgt ttaaaagtct cgtcgttttggccttgttac cgttgatccc 480 ggctgccagc acgtactggg caattaccct gcatggtacggatacgactt acgctttgct 540 gctgtgggtt cgcacctatg ctttcaccgc gttgggattggtgtttctta tcggagttga 600 cttagagacc ttgctgttat ggctggagca gcataaattatcccctaatt ttgtttacgg 660 attactggtt gtgatccatg ccttgccgca aatcatgcatgaagtggctg ctattcgtga 720 agcgagtctg ttacgtggcc aaaagttgca cgcgtggtcgccgatgattt atgtgaaggt 780 gatttttgtt gccatgtcgt ggcaggacca gtacgtcaaagctatgtatg cccatggtta 840 taccgaagga gcagcgcgaa cggttcacca aaccatccgtagttcatggc gcggcttgat 900 cgccatggtg gggggatttg tccttttaaa tctaattgaccgttagtttg aggatcaaaa 960 agaggtggtc gcgcggtgtt taagcgattg tgcagggac 99955 846 DNA Lactobacillus rhamnosus 55 tttgctttac aaaaaccagc atagcgatcattccttaatt accagcataa tcaatcctta 60 attaatccta aattgtgaaa aggtgcattaatagcttaca ctatcattgg aggaaaggag 120 tgacgatgat ggcgaagata ttaattgttgaagatcatag gatatccagg cacttattga 180 aagatgtact aaccccgact tatacggtcactcaggctta tgacggcatt caggctttaa 240 cggcctttca tcgagaacaa ccggacttgatcattctcga tttaatgctg cccaacgtga 300 ctggtgaaag tgttttaaca accattcgtaaaacatccca agttcccgtg ctggtgttaa 360 cggcgattca ggaaaaagcc aaaaccgttgccctgctgca gcaaggcgca aacgattatt 420 tgaccaaacc gtttgacatt gacgaattactagcacgcat tcaagtccaa ctgcgccaag 480 tcagcggcca accaataacg acgaacgatcaactaaaagt cggcgaaatt caattagacc 540 ctaagcgtca tgtggtgacc gttaatcagcaaaccctaac gctgcctaaa aaagaatatg 600 acatgttggc gttaatgatg cgtgatccccatcaagtctt tgataaaagc caactttatg 660 aacatgtatg gggggagccg tttttaaatgccgataatac cctaaacgtt cacatcagca 720 acttacggac aaaaatcaat gaacttgcccatgatcccaa atacatcatt tcaatctggg 780 gcatcggtgt acgtttgatt tagcaaggagaaatcattat gcttggcttc cttattttac 840 ttatcg 846 56 780 DNA Lactobacillusrhamnosus 56 tacgatttgc ttcagttcct caagatccgg ataacttggc aggaattgattccaaccgca 60 ttgccaagta tcaagaagcg ttcgcaaaag cctacaaacg actcatggaagcaatcagtt 120 ccatgagcat tagctggacg attatcggtg ctgcaagtcc gcgctgggctcaaaaagttt 180 tccctgatgc cgccacccct gaagaagcaa ctgagctact ctgggaggcaattttcaaaa 240 ctacccgaat cgatcaaccc gatccggaag ctgcctggaa agcccacgatcaaaagctgc 300 gggaaaaagc ggcctggtta aacaacgaac aatttgatca gctgcattacatggctccgg 360 gaacggattt ggtagtcggt ttaccaaaga accacatttg ggaaggcgccggcgctttta 420 acccgcgtgg tgaggaattt atggctaaca tgccaaccga ggaagtcttcaccgcccctg 480 attttcggcg catcgacggg accgttgctt ccaccaaacc gcttagttatggcggcaaca 540 tcctcgaaga tatgcacttt actttcaaag acgggcaaat cgtggaagcccacgccaaac 600 aaggcgatga cgtcttacaa aacctgctaa aaacgccggg tgctcgttcattaggcgaag 660 tgtcgttggt tccggatcct tcttccatct cacaatcggg ccttattttcttcaatacgc 720 tggttgacga aaatgcttcc gatcatatgg cactcggtca agcctatccgttctcagtca 780 57 1569 DNA Lactobacillus rhamnosus 57 ttgcatcccgctctgctgcc tagttttccc ggacggcagg gcattaaaga tgcgtttgat 60 tatggcgtcaaagtgaccgg cgtgacggtt cattacgtag atgccggaat cgacaccggc 120 gaaatcatcgcacaggatcc ggtccgcgta agcccgggga tgacgctggc acaattagaa 180 gcagccattcaccatcagga acatcaaact tttcccgcaa ctgtcaagca actcattgaa 240 gaaggagcgatttaagtgaa gcgtgcatta ttaagtgtct ctgataaaac tggtttggta 300 ccttttgcaaaaggtctggt tgaacgcggt tttgaactga tttcgaccgg tggtacccat 360 cgcgcactggctgaagctgg ggttgcggtg accggcgtag aagcagtaac cggttttcca 420 gaaatgctcgatggtcgagt taagacactg catcctaaga tccatgccgg catcttggcg 480 cggcgggatgatcctgccca tatgcaggca ttggcagatc atgatattca gccaattgat 540 gtggtttgtgtcaatcttta tccgtttgcc gcgaccattc agcgtgcggg tgtgacgcgt 600 gccgaggcgattgaacaaat tgatattggt ggcccgtctg cgttacgtgc ggcagctaaa 660 aatagtgacagcgtctgggc cgtggttgat ccggcagatt atgcggatgt tttagccgga 720 ctcgatcaaaatgatgctga tttacggcaa cggttggcag ccaaagtctt ttccgccact 780 gccgcttacgatgctcaaat cgcccattac ttagatccag agcctttccc agagcagttc 840 acgccaacttatcacaaacg gcaggacttg cgttacgggg aaaacagcca ccaacaagct 900 gccttttatgttgagccgaa tcctgatcca accagtcttg ccgctgctaa gcaattacac 960 ggtaaagagctttcttataa caatatcaag gatgcggatg cagcattggc aatgttgcgg 1020 gaattcaaacaaccggcagc cgtggccgtt aaacatatga acccatgcgg tattggcttg 1080 ggcgatacgcttgaagccgc ttgggacaag gcatatgccg ccgatccgat gtccattttt 1140 ggcggcatcattgctttgaa tcggcgggtt gatcttgcca ctgccgagaa aatgcataaa 1200 cttttccttgagatcatcat ggcaccggca tttgatgatg atgcttatga gattctggcg 1260 aagaagaaaaatgtgcgctt attgacgatt aataccgccg atacgccaga agaattggga 1320 acagaaacaacttcaattta tggtgggttg ttgattcaaa cacgtgacga caaagctgag 1380 acaccagccgatatgacggt ggtgacggag gtcaagccga ctgaagctca actcaaggca 1440 ttggcatttgctcagacggt ggtcaaacat gttaagagta atgcgattgt cgtggctcag 1500 gctgatcagacgttagggat tggcgccggt caaatgaatc ggattggctc ggttgaattg 1560 gcgttaacc1569 58 1112 DNA Lactobacillus rhamnosus 58 gatcgtgtca gtggctttagtggcaggttg tgttggcctg catcgattcg ttgctgaact 60 tgttcttccg ataacccttgatcaggtgaa atcttttgct ctgccatcgc atgccctcca 120 gtacttttca tccattttaccaatccagac aacaagatgc ccgaatatca cacttgtttt 180 tacaaaactt tgaagttgttggcgtggttg ggttatggtt agggagtcat gaaaccgtca 240 taatgagatg aaagttatagaaagaagtgc tctcatggta aaacgaaacc caaatggaac 300 ccgatttatc acattacctaatggctacca cttgtggacc cagacattag cagcggccga 360 ttcattactg acgttgcacggcggaccagg cggcacgaat gaagtgtttg aaaatttcgc 420 tactgaactg gcatcttttggtgtccgtgt ctcacggtac gaccaactgg gttcattttt 480 ctctgatcaa ccggacttttccgatccggc taatcaaaag cgttttctca atatcgccta 540 ttatgttgac gaagtggaaaatgttcggca acagctgggc cttgatcatt tttacctatt 600 aggtcagtcc tggggcggtgtgttggcgat tgaatatggc ttgaaatatt cgcagcatct 660 taagggactt attttgagctcgatgattga taatttggat gagtatttgg ttaacattaa 720 caagattcgg gagaccatgttttctagtga tgacgtggca tacatgcaac ggattgaagc 780 ccagcacgcg tttacggatgccaaatacca gcaattggtg cgtgagttag gggagcaata 840 tcttcatcat gccaaagatccccagccgcg ccatttaatt agcacgttgg cgacccccgt 900 gtatcatcat tttcaaggtgataatgaatt tgtgatggta ggcgcactta gggactggga 960 tcgacgggct gatatacaccgtctgacaat gccgacttat ctgacattcg gtggacatga 1020 aaccatgccg ttatcagctgccaagcgaat ggctcggaca ataccaaatg ccactttgca 1080 tgtcacacct aatgccggtcatggtcagat gt 1112 59 1570 DNA Lactobacillus rhamnosus 59 ataggggatgagtaggcgat ataggcagtt tgatgctaac cgcgccggct cgcgctcacg 60 aggaggattaaatttgattt cactaggaat ttacgaaaaa gcactgccca ggaccgaatc 120 ttgggttgaacggttgaaaa tggttcggga tttgggtttc aactttttgg agttatcggt 180 tgatgaaagtgacgaacggt tggcccggtt ggattggacg gcagcgaaac gggcgaaggt 240 gcgtgatgcttgttggcaaa caggggtgcg gatccataca ttgatgttaa gcggccatcg 300 tcggtttccattaggctcgg cagacccggc gatccgtgaa aaaagtctga cgatgttatg 360 taaggcgattgatttggcta gtgatctggg cgttcgcaat gttcaactag ccggttatga 420 cgtttactatgagccgaaaa ccttagcttc acgagaatat ttcatcgaga atttgaagcg 480 cggtgtggcctatgctgcgg ctaaagaagt gatgctggca attgagacga tggacgatcc 540 gtttttgaattcactttcca agatcaagac gattaaagat gagattccga gtccgtggtt 600 gcaagcatatccggatctgg gcaacttgtc agcctggccg gaaaacaatg tcggtcgcga 660 gcttgaactgggaattgcaa acatcgtctc ggttcatttg aaggataccc aagcggtgac 720 ggtaaaaagcaaagggcaat tccgcgatgt cccgtttggc gctggcgtgg tggacttttc 780 cggctgcctgcgtacgctca aacgccttga ctacagcggt gcctttacga tcgagatgtg 840 gacggaaaaggccgctgatc caatccaaga agtgaagcag gccaaggact ttttcgatcc 900 gctgtttgtgcaggccggtt ttgttcagga gccagtggca aaaaccaatg tcccatcatg 960 aagcgcctgctgacctgcac acgaaaaaag tcgccaattg cttgccggct ttagacgatt 1020 catttaactataggcgaatg gcagtggggc tgatctggaa gtaatcgatt aaatagttag 1080 caaccccgtcatcatcattt gagcccacgg taatgtccgc aatttgtttg atttcaggaa 1140 tcgcgttacccatggccacc ccgatgccgg catactccag catgtctttg tcattttcct 1200 catcgccaaatgcggccagt tgttcgcgcg ggatgcggta aaagttgagt gcggctttta 1260 aaccgttcatcttattgaca tttggcggca ggatttcaag cagtgtgcgc cgggatcgcg 1320 tgacggtcagctgcttgagt gactgggcag cgtctgatgc ggcatcgatc taagtgcaac 1380 gcgattggcaaatcagtatg atttgccgca tcaataactt gtgccacaaa actgtcgcgg 1440 agaaattctaattctgtggg atgaatcgcc ataataaatg gtgcctgcag ttcttccgcc 1500 ttttccaacgtggcagtgaa gagttgtcct gagcctgcgt tgaaagcagg aatggcaaaa 1560 tggtgttcct1570 60 948 DNA Lactobacillus rhamnosus 60 aactagtgga tcaaacatgacagatcccat tgcgtttttg caaaaactaa tccaaattga 60 ctctgcaaat ggaaacgaacttgcagtagc ccgcgttttg caagctgaac tcgaagcggc 120 cgatattcca accaaattgatcccatacaa agatgatcgg gtcaatttag tcgcccagct 180 caatcacggt gaccgcgtattaggcttcac cggccatgaa gacgtggtga gtcccggcga 240 tgagaacgcc tggacctatccgcctttttc cggaaagatc gtgaacaaca ccatgtacgg 300 tcgcggcacc gatgatatgaaaagtgggct agcagccatg accttggcac tgatccacct 360 taagcaaagc ggctttgcccatccgctgcg tttcatggcc acggtcggtg aagagtttgg 420 tgcaatggga gcgcgccaactcaccgaaca aggttatgcc gatgacttaa ccggactcgt 480 ggtaggcgaa ccaacgaacaaattgctaaa atacgcacac ggcggaacgg ttaactacga 540 aattgacagc gaaggcgtttccgtccacag ctcccggccg gaaaaaggcg ttaacgcaat 600 tgaagggttg gtggcattttctactcccga accgcacgcc tttgatcagg cccctgatga 660 tcctgacctt ggtccattccgccactccat taccgtgatc aaaggcggcg atcaagtcaa 720 caccattcct gcccatgcatacttacgcgg caacttgcgc ccgactcctg cagcaaatat 780 cgaattagtc gtcgggttattggaaaaatt agtcgatcag gcaaataaag ccaccgccgc 840 taacttgacg ttgaacgttttacatcgttt tttgccggta cactctgaca aaaacgggca 900 tctcgtgaca accgctaacgaagccattgc cgctgtgact ggtaagcc 948 61 1188 DNA Lactobacillus rhamnosus61 ggttagaagg aattggcgag tttgcgcagt gaatgctttt gtattccaat atcatcgcca 60aagcctgatt aataaatcga ttttgtatct taaataataa atatgtacac gttttcatga 120atatgagaac gtgtttttta tttaaaaaag ataaagcgct tgcataagag ataaatggct 180tttatattta acttgttcac agaggtaccc tgtgatacac ggttgttcta tgatgttcgt 240aaactaatag aaagatggcg ggaaaatgaa aattgatatt gacaaaacgt ctatgattcc 300agtttacgaa caaattgcaa atagtttgcg agacatgatg tatggcggaa gtctacagga 360tggagaccgt ttagactctg agcagaagat gtgtcgcaac cttaatgtca gccgtggaac 420tgttagaaaa gctattgata ttctactgaa ggagggtatg gtcaaaaaga ttcatgggaa 480aggaaccttt gtcagtaacc caaacgttga gtactcgttg aatgatcagt taatgtcatt 540cgctgagtcg ctcgataatc aacacttaag ttacacaaca caagttattc agcaggaact 600acgacccgcg accgcgaaaa ttgctgatat gctcaagatc cctattgata gtcagtactt 660gtatttagaa cgattgcgat cagttgccga tgataagtta atgttaatcg aaaatcgcat 720taatattacg ctctgtccgg gaattgagaa ggtcaatttt aacaacatta gcctttttaa 780tgagattgaa gaactagcaa aaagaaagat tagctttgcc cgcagtacct atgaagcact 840tacgattggc acggaacgtg ggaaactttt agagcttcct tcttccacgc cggctttaaa 900aatgcaacag acagtgtatc tttctgaaaa agaaccagtc gaatacggtt ccgtctggtt 960aaaaggaaat aagtactttc tcacaacaac tttgcaaaga cgataggagg aattgaacca 1020atgccattag taaatggatt cgatttgatc aaaattatta aggatcggca cgttgtcgca 1080ggagctttca acacaactaa tctggagaca accatgggta tacttcgagc ggttgaaaaa 1140agcggcatac catctttcat tcaaattgcc ccgacaaaca tccccgtt 1188 62 1637 DNALactobacillus rhamnosus 62 ctagtggatc ccttcttatt tacaccgcaa ctgaccatcgaagaagtcaa aaaagccggc 60 tgggcctacc cggtattcgg ttatcttgat cacgaggacccgtttgcaaa actggcgagt 120 catattaaaa ccgtcaaccc taatccgaca aaatgggcaatcgaaaaaga taatctcgcc 180 gttttcaaat ttgaagcgat tatgaagcag ttcccggacgctaccttccc gattgatgct 240 tctcgtttta ttgaaaaaca gcgcctgatc aaaaccgcttcagagatcaa acagatggaa 300 gccgctggtg ctcaagccga tcgggcattt caggcaggattcaatgccat taaagccgga 360 gcaaccgaac aagaagtcgc cgctgaaatc gattatgccatgatgaaaga aggcgtcatg 420 cacatgagct tcggcaccat tgtccaagct ggtgtcgatgctgccaaccc gcatggcgaa 480 ccgatgggaa caaaactcgc acctaacgaa ttggttttgttcgatctggg caccgacaat 540 catgggtata tgtccgatgc gacccgcaca gttgcttttggtcaagtcac tggtaagcca 600 cgggaaattt ttgacatctg tcttgaggct aatttaaccgcgatggacgc tgttaagcca 660 ggacttaaag catccgaact ggacaaaatt gcgcgtgatatcattactaa agcgggctat 720 ggcgagtact tcaatcatcg gctcggccac ggcatcggtatgtcgacgca cgaattcccg 780 tccatcatgg aaggcaatga tatgatcgtg ggcgaagattttgggatgcg tgtcagtgtc 840 cttgccagca gcagctctgg taacgcaacc tatattgaaacgcctggtca caaggtgtta 900 gtggatgccg gtttatcagg caagaaaatt gaagcgctgatgaagagcat cggcagagat 960 ctaaccgatg ttgacagtgt ttttatcacg catgaacatagcgatcatgt gcgtggtgta 1020 ggcgtgttgg cgcgacgtta tccgcagctc aacgtttatgcgaatgccaa gacgtttgca 1080 gctttaccaa aaagtgtggg caaaattcct gaagcacagctgcggttgtt tgatatgggg 1140 acaactttga cgttaggtga tttagatgtg gaaagttttggcgtttcgca tgatgctgcc 1200 gcgcctcagt tttaccaatt tcatcatgac ggcaaggccttcactatcct aaccgacacg 1260 ggctacgttt cagatcgggt tgccgggacg attcgcgatgccgatgcgta tgtgatggaa 1320 tgcaatcacg accttgaaat gttgcggaca ggtccgtatccgtggccgtt gaagcaacgg 1380 attttaagcg atcaagggca cctgtccaat gaggatggtgcggatgcttt gatggatgtc 1440 atcggtttgc ggacgaagcg gatttatttg gggcatttatcaccgcataa caacaataaa 1500 gccactggcg catttaaccg tggcgtcgtt gttggcacaacaaggtctgg cggtggatca 1560 tgactttcat atttatgaca ctgacccggc agttgccgacccattgtttg ttgtgtgaag 1620 cggtttgaaa gcagttt 1637 63 224 PRTLactobacillus rhamnosus 63 Leu Arg Gly Leu Cys Ile Gly Ile Val Ala CysGlu Phe Phe Glu Ile 1 5 10 15 Pro Leu Thr Pro Ser Glu Ser Ala Asp AsnGly Ile Gln Lys Arg Asn 20 25 30 Asp Val His Gln Trp Leu Val Ile Phe ArgArg Asp Leu Leu Ala Asp 35 40 45 Leu Lys His Phe Asp Asn Gly Asp Arg GlySer Lys Gly Cys Val Phe 50 55 60 Asp Gln Ala Asp Glu Thr Ile Gln Trp ArgAsp Gly Arg Ser Cys Leu 65 70 75 80 Arg Asn Asn Asp Phe Ala Gln His GlnSer Pro Arg Gln Ser Asn Cys 85 90 95 Ile Ser Arg Phe Pro Leu Pro Gly IleAsn Arg Gln Gln Arg Gly Ala 100 105 110 Gly Arg Phe Gly Thr Ile Arg ProArg Val Lys Glu Cys Tyr Asn Ser 115 120 125 Arg Gly Arg Gly Ile Leu AspAla Met Arg Glu Asn Thr Arg Asp Asp 130 135 140 Glu Ala Gly Ala Glu GluAsn Asp Glu Leu His Gln Gln Arg Arg Ala 145 150 155 160 Lys Glu Pro AsnVal Lys Asn Gly Asp Ser Phe Cys Asp Ser Asp Gln 165 170 175 Asn Thr PheThr Asn Arg Tyr Ala Arg Gln Cys Gly Tyr Lys Cys Asp 180 185 190 Asp GlnAla Asp Arg Lys His Glu Cys Asn Trp Asn Gly Val Phe Asn 195 200 205 AlaGly Cys Tyr His Leu Arg Asn Cys Ile Gly Asn Asn Phe Pro His 210 215 22064 475 PRT Lactobacillus rhamnosus 64 Leu Ile Cys Lys Gly Arg Ser LeuLys Pro Phe Gly His Phe Ile Asp 1 5 10 15 Ala Ile Thr Val Asn Arg GluHis Val Leu Thr Thr Ala Ala Glu Ala 20 25 30 Leu Ile Ala Ser Ala Gly AspAla Leu Asn Ala Ser His Ala Thr Phe 35 40 45 Asn Val Leu Asn Asn Ser AspLeu Gln Phe Gly Phe Val Glu Asn Glu 50 55 60 Asp Gly Glu Thr Val Gln LeuSer Asn Gly Leu Tyr Gly Gln Leu Ile 65 70 75 80 Arg Ser Thr Asn Arg LysLeu Arg Lys Glu Ala Phe Glu Ala Leu Leu 85 90 95 Arg Ala Tyr Glu Ser LeuLys Asn Thr Phe Ala Gln Thr Leu Ser Gly 100 105 110 Gln Val Lys Ala HisAsn Phe Asn Ala Thr Ala His His Tyr Lys Asn 115 120 125 Ala Arg Ala AlaAla Met Ala Ser Asn His Ile Pro Glu Ser Val Tyr 130 135 140 Thr Thr LeuIle Asp Gln Val Asn Thr His Leu Pro Leu Leu His Arg 145 150 155 160 TyrVal Ala Leu Arg Lys Lys Val Leu Ala Val Asp Gln Leu His Met 165 170 175Tyr Asp Ile Tyr Thr Pro Leu Thr Gly Gln Pro Pro Leu Thr Tyr Thr 180 185190 Leu Glu Gln Ala Lys Ala Glu Ala Leu Lys Ala Leu Ala Pro Leu Gly 195200 205 Asp Asp Tyr Leu Glu His Val Arg Glu Ile Phe Asp Asn Arg Tyr Ile210 215 220 Asp Val Val Glu Asn Lys Gly Lys Arg Ser Gly Ala Tyr Ser GlyGly 225 230 235 240 Ala Tyr Asp Thr Asn Pro Phe Ile Leu Leu Asn Trp HisAsp Ala Val 245 250 255 Asp Glu Leu Tyr Thr Leu Val His Glu Thr Gly HisSer Val His Ser 260 265 270 Trp Tyr Thr Arg His Asn Gln Pro Tyr Val TyrGly Asp Tyr Pro Ile 275 280 285 Phe Val Ala Glu Ile Ala Ser Thr Thr AsnGlu Asn Leu Leu Thr Asp 290 295 300 Tyr Phe Leu Thr His Ser Asp Asp ProLys Val Arg Ala Tyr Ile Leu 305 310 315 320 Asn Tyr Tyr Leu Asp Gly PheLys Gly Thr Val Phe Arg Gln Thr Gln 325 330 335 Phe Ala Glu Phe Glu HisTrp Ile His Gln Gln Asp Gln Gln Gly Glu 340 345 350 Pro Leu Thr Ala ThrSer Met Ser Gln Tyr Tyr Ala Asp Leu Asn Ala 355 360 365 Arg Tyr Tyr GlyPro Glu Val Ala Arg Asp Pro Glu Ile Ala Phe Glu 370 375 380 Trp Ala ArgIle Pro His Phe Tyr Tyr Asn Tyr Tyr Val Tyr Gln Tyr 385 390 395 400 AlaThr Gly Phe Ala Ala Ala Ser Thr Leu Ala Ala Gly Ile Ser Ser 405 410 415Gly Glu Pro Asp Ala Ala Ala His Tyr Leu Asp Tyr Leu Lys Ser Gly 420 425430 Ser Ser Lys Tyr Ala Ile Asp Thr Met Lys Thr Ala Gly Val Asp Met 435440 445 Thr Lys Pro Asp Tyr Leu Glu Ala Ala Phe Ser Val Phe Glu Gln Arg450 455 460 Leu Thr Glu Leu Glu Lys Ile Leu Gln Lys Gly 465 470 475 65345 PRT Lactobacillus rhamnosus 65 Glu Glu Leu Gly Leu Pro Gln Leu ValArg Met Ser Ala Asn Glu Asn 1 5 10 15 Pro Phe Gly Thr Ser Val Lys ValGln Gln Ala Val Thr Asn Trp Asn 20 25 30 Phe Thr Gln Ser Arg Asp Tyr ProAsp Gly Tyr Ala Ser Gln Leu Arg 35 40 45 Thr Ala Val Ala Lys His Leu AspVal Ala Ala Glu Gln Leu Val Phe 50 55 60 Gly Asn Gly Leu Asp Glu Val IleAla Leu Ile Ala Arg Thr Phe Leu 65 70 75 80 Ser Pro Gly Asp Glu Val IleGlu Pro Trp Pro Thr Phe Ser Glu Tyr 85 90 95 Arg Leu His Ala Gln Ile GluGly Ala Thr Val Ile Asp Val Pro Val 100 105 110 Thr Glu Thr Gly Asn PheAsp Leu Ser Ala Met Ala Gln Ala Leu Thr 115 120 125 Ala Lys Thr Lys LeuIle Trp Val Cys Asn Pro Asn Asn Pro Thr Gly 130 135 140 Thr Leu Leu SerIle Ala Thr Leu Thr Glu Trp Leu Arg Gln Ile Pro 145 150 155 160 Lys AspVal Leu Val Leu Met Asp Glu Ala Tyr Ile Glu Phe Thr Asp 165 170 175 AspTyr Pro Ala Thr Ser Ala Ile Ser Leu Leu Ser Lys Phe Pro Asn 180 185 190Leu Val Val Leu Arg Thr Phe Ser Lys Ile Tyr Gly Leu Ala Asn Phe 195 200205 Arg Val Gly Phe Gly Val Phe Pro Lys Gln Leu Val Asn Tyr Leu Gln 210215 220 Thr Val Arg Leu Pro Tyr Asn Leu Ser Ser Ile Ala Gln Val Ser Ala225 230 235 240 Gln Ala Ala Leu Ala Asp Gln Asp Phe Val Ala Met Thr ArgLys Arg 245 250 255 Val Gln Gln Ala Arg Asp Ser Trp Glu Arg Phe Leu ThrGln Thr Gly 260 265 270 Leu Pro His Thr Arg Ser Gln Thr Asn Phe Gln PhePhe Gln Ala Pro 275 280 285 Lys Met Gln Ala Ser Ala Leu Lys Lys Arg LeuLeu Gln Gln Gly Phe 290 295 300 Leu Val Arg Asp Gly Leu Lys Pro Gly TrpLeu Arg Val Thr Phe Gly 305 310 315 320 Thr Glu Val Gln Asn Thr Ala ValGln Arg Ile Ile Glu Thr Phe Gln 325 330 335 Ala Glu Leu Thr Gly Pro AsnAla Leu 340 345 66 369 PRT Lactobacillus rhamnosus 66 Val His Leu AlaLys Arg Ile Leu Asn Val Ala Pro Ser Ala Thr Leu 1 5 10 15 Ala Leu SerAsn Gln Thr Lys Asp Leu Lys Ala Lys Gly Ala Asp Val 20 25 30 Ile Asp LeuSer Ile Gly Gln Pro Asp Phe Ser Thr Pro Lys Ala Ile 35 40 45 Asp Asp AlaAla Ile Ala Ala Ile Gln Ala Gly Asn Ala Ser Phe Tyr 50 55 60 Thr Ala AlaThr Gly Ile Pro Glu Leu Lys Gln Ala Ile Ser Asp Arg 65 70 75 80 Ile PheAla Gln Asp Gly Ile Arg Tyr Asp His Arg Gln Ile Val Ala 85 90 95 Thr ThrGly Ala Lys Phe Ala Leu Tyr Ala Leu Phe Gln Val Phe Leu 100 105 110 AsnPro Gly Asp Glu Val Leu Ile Pro Val Pro Tyr Trp Val Ser Tyr 115 120 125Glu Glu Gln Ile Lys Leu Ala Ser Gly Val Pro His Leu Val Met Pro 130 135140 Ala Val Gly His Lys Val Ser Val Asp Asp Leu Glu Ala Ala Arg Thr 145150 155 160 Asp Lys Thr Arg Ala Leu Ile Ile Asn Ser Pro Gln Asn Pro SerGly 165 170 175 Val Val Tyr Asp Arg Thr Glu Leu Thr Leu Ile Gly Asn TrpAla Leu 180 185 190 Lys His His Ile Leu Val Val Thr Asp Asp Ile Tyr ArgAsp Leu Ile 195 200 205 Tyr Asn Gly Thr Thr Tyr Thr Ser Met Ile Ser IleAsp Pro Asp Ile 210 215 220 Ala Ala Asn Thr Val Leu Ile Ser Gly Val SerLys Ser Tyr Ala Met 225 230 235 240 Thr Gly Trp Arg Ile Gly Tyr Ala AlaGly Pro Glu Lys Leu Ile Gln 245 250 255 Ala Met Ala Thr Phe Ile Ser HisThr Thr Ser Asn Pro Ala Ala Val 260 265 270 Ser Glu Tyr Ala Ala Val AlaAla Leu Thr Gly Asp Gln Gln Val Val 275 280 285 Glu Lys Met Arg Arg AlaPhe Glu Glu Arg Leu Asn Leu Phe Tyr Asp 290 295 300 Leu Leu Ala Asp IlePro Gly Phe Asp Met Gly Asp Lys Pro Gln Gly 305 310 315 320 Ala Phe TyrLeu Phe Pro Asn Ile Lys Arg Ala Ala Gln Leu Ser His 325 330 335 Tyr GlyThr Val Asp Asp Phe Ile Ser Ala Leu Leu Thr Glu Thr Gly 340 345 350 ValAla Ile Val Pro Gly Arg Ala Phe Gly His Ala Gly Ser Cys Ala 355 360 365Asp 67 390 PRT Lactobacillus rhamnosus 67 Met Thr Leu Gln Pro Leu AsnGlu Gln Leu Pro Ala Ile Glu Val Ser 1 5 10 15 Glu Ile Arg Gln Phe AspGlu Ser Val Ser Asp Ile Pro Gly Ile Leu 20 25 30 Lys Leu Thr Leu Gly GluPro Asp Phe Asn Thr Pro Glu His Val Lys 35 40 45 Gln Ala Gly Ile Lys AlaIle Gln Glu Asn Tyr Ser His Tyr Thr Gly 50 55 60 Met Val Gly Asp Pro GluLeu Arg Glu Ala Ala Gln His Phe Phe Lys 65 70 75 80 Thr Lys Tyr Ala ThrAsp Tyr Arg Ala Thr Asp Glu Ile Leu Val Thr 85 90 95 Val Gly Ala Thr GluAla Leu Ala Thr Ala Ile Thr Thr Ile Ser Asp 100 105 110 Pro Gly Asp AlaMet Leu Val Pro Ser Pro Ile Tyr Pro Gly Tyr Ile 115 120 125 Pro Leu LeuThr Leu Asn His Val Thr Pro Leu Tyr Met Asp Thr Ser 130 135 140 Lys ThrAsp Phe Val Leu Thr Pro Glu Leu Ile Glu Ala Thr Ile Thr 145 150 155 160Ala Asn Pro Asp Ala Lys Ile Lys Gly Ile Ile Leu Asn Tyr Pro Ser 165 170175 Asn Pro Thr Gly Val Thr Tyr Arg Ala Ala Glu Val Lys Ala Ile Ala 180185 190 Asp Ile Ala Ala Lys His Asn Leu Tyr Ile Ile Cys Asp Glu Ile Tyr195 200 205 Ser Glu Leu Thr Tyr Gly Glu Pro His Val Ser Met Gly Gln PheAla 210 215 220 Tyr Asp Arg Thr Phe Ile Val Asn Gly Leu Ser Lys Ser HisAla Met 225 230 235 240 Thr Gly Trp Arg Ile Gly Phe Leu Met Gly Pro GlnGln Leu Ile Ala 245 250 255 Gln Ala Lys Lys Val His Gln Tyr Leu Val ThrAla Ala Thr Thr Ile 260 265 270 Ala Gln Arg Ala Gly Ile Glu Ala Leu ThrAsn Gly Ala Asp Asp Ala 275 280 285 Gln Val Met Lys Ala Ala Tyr Val LysArg Arg Asp Phe Val Tyr Ala 290 295 300 Ala Leu Ile Asp Met Gly Phe SerVal Ala Arg Pro Asp Gly Ala Phe 305 310 315 320 Tyr Leu Phe Ala Lys IlePro Thr Gln Leu His Leu Ser Ser Arg Glu 325 330 335 Phe Thr His Ala LeuAla His Glu Gln Lys Leu Ala Leu Ile Ser Gly 340 345 350 Thr Ala Phe GlyPro Gly Gly Glu Gly Tyr Ile Arg Ile Ser Tyr Ala 355 360 365 Ala Ser MetThr Asp Leu Gln Glu Ala Val Lys Arg Leu Arg Ala Phe 370 375 380 Met AlaSer His Ile Gly 385 390 68 297 PRT Lactobacillus rhamnosus 68 Val AlaArg Leu Met Leu Asp Pro Gly Asp Gly Leu Val Val Glu Ala 1 5 10 15 ProThr Tyr Leu Gly Ala Leu Ala Ala Phe Asn Ala Tyr Gln Pro Thr 20 25 30 TyrTyr Glu Ile Pro Met Gln Asp Asp Gly Met Asp Ile Asn Ala Leu 35 40 45 GlnArg Val Leu Met Ser His Lys Val Lys Phe Ile Tyr Thr Val Pro 50 55 60 AspPhe Gln Asn Pro Thr Gly Val Val Met Ser Val Ala Lys Arg Gln 65 70 75 80Ala Leu Ile Arg Leu Ala Asn Gln Tyr Asp Val Met Ile Leu Glu Asp 85 90 95Asn Pro Tyr Arg Asp Leu Arg Tyr Asp Gly Lys Pro Leu Pro Thr Ile 100 105110 Lys Ser Phe Asp Thr Gln Gly Arg Val Val Tyr Leu Gly Ser Phe Ser 115120 125 Lys Ile Leu Ser Pro Ser Leu Arg Met Gly Trp Leu Val Ala Ala Pro130 135 140 Asp Leu Leu Gln Glu Leu Leu Ala Leu Lys Gly Gly Ser Asp LeuGlu 145 150 155 160 Ser Ser Asn Leu Thr Met His Gly Ile Asp Ala Tyr MetAla Glu Asn 165 170 175 Asp Leu Asp Ala His Ile Thr Glu Ile Gln Asn CysCys Arg Glu Lys 180 185 190 Lys Asn Ala Met Val Ala Ala Met Asn Arg TyrLeu Pro Asp Glu Ala 195 200 205 His Phe Thr Asn Pro Asp Gly Gly Phe PheLeu Trp Leu Thr Met Pro 210 215 220 Ala Gly Phe Asp Met Gly Ala Phe MetLys Gln His Leu Leu Pro Glu 225 230 235 240 Ser Asn Ile Ser Tyr Val ProSer Ala Asn Leu Tyr Ala Thr Ser Ala 245 250 255 Gln Val Asn Gly Ala ArgLeu Asn Phe Thr Gly Pro Thr Leu Glu Gln 260 265 270 Ile Asp Thr Gly IleLys Ala Leu Gly Asp Ala Leu Lys Thr Ala Leu 275 280 285 Gln His His LeuVal Ala Glu Gln Ala 290 295 69 390 PRT Lactobacillus rhamnosus 69 MetLys Leu Thr Ile Tyr Asp Phe Asp His Val Ile Asp Arg Arg Gly 1 5 10 15Thr Phe Ser Thr Gln Trp Asp Tyr Ile Ala Asp Arg Phe Gly Arg Asn 20 25 30Asp Ile Leu Pro Phe Ser Ile Ser Asp Thr Asp Phe Pro Val Pro Val 35 40 45Glu Val Gln Asp Ala Leu Lys Glu Arg Leu Thr His Pro Ile Tyr Gly 50 55 60Tyr Thr Arg Trp Asn His Ala Thr Tyr Lys Asp Ser Ile Val His Trp 65 70 7580 Phe Glu Arg Asp Gly His Thr Lys Ile Asn Pro Asp Trp Ile Val Tyr 85 9095 Ser Pro Ser Val Val Phe Thr Ile Ala Thr Leu Ile Arg Met Lys Ser 100105 110 Asp Pro Gly Asp Gly Val Ala Val Phe Thr Pro Met Tyr Asp Ala Phe115 120 125 Tyr Gly Thr Ile Lys Gln Asn Asp Arg Val Leu Ile Pro Ile ArgLeu 130 135 140 Ala Ala Ala Asp Glu Gly Tyr Val Ile Asp Trp Asp Ser LeuAla Thr 145 150 155 160 Val Leu Ala Glu Lys Gln Thr Lys Ile Phe Leu LeuThr Asn Pro His 165 170 175 Asn Pro Thr Gly His Val Phe Thr Lys Ser GluLeu Ala Arg Leu Tyr 180 185 190 Asp Leu Cys Gln Ala Ala His Val Phe LeuIle Ser Asp Asp Ile His 195 200 205 Arg Asp Ile Val Tyr Pro Gly His SerTyr Glu Pro Met Thr Asn Val 210 215 220 Gly Thr Ser Asp Val Ala Leu CysCys Ser Gly Ser Lys Thr Phe Asn 225 230 235 240 Thr Pro Gly Leu Ile GlySer Tyr Ala Phe Leu Pro Asp His Asp Val 245 250 255 Arg Ala Gln Phe LeuThr Glu Leu Lys Gln Lys Asn Ala Leu Ser Ser 260 265 270 Val Ser Ile PheGly Met Leu Ala Gln Ile Ala Ala Tyr Asn Gly Ser 275 280 285 Glu Asp TyrVal Glu Gln Leu Thr Ala Tyr Thr Lys Asn Asn Met Glu 290 295 300 Leu ValAla Ser Tyr Leu Glu Glu Asn Leu Pro Glu Leu Gln Phe Ser 305 310 315 320Leu Pro Asp Ala Thr Tyr Leu Ala Trp Ile Asn Val Ser Lys Leu Arg 325 330335 Leu Thr Ser Glu Glu Leu Gln His Arg Leu Val Asn Gly Gly His Val 340345 350 Gly Ile Met Ala Gly Lys Thr Tyr Gly Asp Thr Arg Tyr Leu Arg Met355 360 365 Asn Ile Ala Cys Pro Lys Lys Lys Leu Val Met Gly Leu Glu ArgLeu 370 375 380 Lys Lys Gly Ile Arg Gly 385 390 70 386 PRT Lactobacillusrhamnosus VARIANT (1)...(386) Xaa = Any Amino Acid 70 Met Ile Tyr PheAsp Asn Ser Ala Thr Thr Lys Ile Ser Pro Asp Ala 1 5 10 15 Leu Ala ThrTyr Asn Lys Val Ser Thr Asp Phe Phe Gly Asn Pro Ser 20 25 30 Ser Leu HisAla Leu Gly Thr Lys Ala Asn Glu Val Leu Gln Ser Ser 35 40 45 Arg Ala GlnIle Ala Lys Leu Ile Gly Ala Lys Pro Asp Glu Ile Tyr 50 55 60 Phe Thr SerGly Gly Thr Glu Arg Asp Asn Trp Val Xaa Leu Lys Gly 65 70 75 80 Thr AlaTrp Leu Asn Ala Asn Leu Ala Arg Ile Leu Ile Thr Thr Ser 85 90 95 Ile GluPro Pro Ala Val Ile Asn Thr Met Lys Gln Leu Glu Lys Leu 100 105 110 GlyPhe Glu Val Thr Tyr Leu Pro Val Asp Arg Arg Gly Phe Ile His 115 120 125Ile Asp Asp Leu Lys Ala Ala Ile Arg Lys Asp Thr Ile Leu Val Ser 130 135140 Ile Met Ala Val Asn Asn Glu Ile Gly Ser Met Gln Pro Ile Val Gln 145150 155 160 Ala Ala Arg Val Leu Asp Asn Tyr Pro Asn Ile His Phe His ValAsp 165 170 175 Ala Val Gln Ala Val Gly Lys Gly Leu Asp Ala Ala Leu GlnAsp Pro 180 185 190 Arg Ile Asp Phe Leu Ser Phe Ser Gly His Lys Phe HisAla Pro Arg 195 200 205 Gly Thr Gly Phe Ile Tyr Ala Lys Glu Gly Arg MetLeu Asp Pro Leu 210 215 220 Leu Thr Gly Gly Gly Gln Glu His Asp Trp ArgSer Gly Thr Glu Asn 225 230 235 240 Val Pro Ala Ile Ala Ala Met Ala LysSer Leu Arg Leu Leu Leu Ala 245 250 255 Asn Glu Asp Ala Asn Val Ala ArgGln Gln Ala Val Arg Lys Arg Ile 260 265 270 Phe Glu His Val Ser Gln LysPro Lys Val Thr Met Phe Ser Gln Leu 275 280 285 Thr Pro Asp Phe Ala ProHis Val Leu Cys Phe Ala Ile Ala Gly Val 290 295 300 Arg Gly Glu Thr IleVal His Ala Phe Glu Asp His Gln Ile Tyr Ile 305 310 315 320 Ser Thr ThrSer Ala Cys Ser Ser Lys Lys Gly Thr Glu Ser Ser Thr 325 330 335 Leu AlaAla Met His Thr Asp Pro Lys Ile Ala Thr Ser Ala Ile Arg 340 345 350 ValSer Leu Asp Glu Ala Asn Thr Leu Asp Glu Ala Asp Ala Phe Asn 355 360 365Ala Ala Phe Asp Thr Ile Tyr Ala Lys Phe Ala Lys Leu Asp Lys Ala 370 375380 Thr Val 385 71 262 PRT Lactobacillus rhamnosus 71 Met Pro Thr LysIle Gly Leu His Tyr Asn Lys Ile Gly Val Gly Lys 1 5 10 15 Thr Ile TyrPhe Leu His Gly Met Gly Leu Asp Gly His Ser Met Ala 20 25 30 Ala Phe TyrGlu Pro Arg Phe Thr Ser Glu Glu Arg His Phe Ala Arg 35 40 45 Leu Tyr ProAsp Leu Pro Gly Met Gly Asn Ser Pro Ala Thr Ser Ala 50 55 60 Leu Gln SerAla Asp Asp Val Leu Ala Gln Val His Ala Phe Ile Gln 65 70 75 80 Ala ThrSer Glu Gly Pro Cys Tyr Leu Val Gly His Ser Tyr Gly Gly 85 90 95 Tyr LeuAla Leu Gly Leu Leu Ala Arg Phe Pro Asp Glu Phe Ser Gly 100 105 110 AlaPhe Leu Thr Ala Pro Val Val Leu Ala Glu Lys Thr Ala Arg Thr 115 120 125Val Ala Thr Leu Lys His Leu Ile Ser Ala Pro Val Thr Ser Gln Ser 130 135140 Pro Glu Phe Thr Asp Tyr Gln His Met Asn Val Val Ile Asn Pro Ser 145150 155 160 Thr Trp Arg Gln Tyr Gln Glu Leu Ile Leu Pro Gly Leu Lys ThrPhe 165 170 175 Asn Arg Asp Phe Trp Val Ala Met Lys Asn Arg His Ala TyrArg Leu 180 185 190 Ser Ile Glu Ser Arg Leu Thr Ser Leu Ile Lys Ser ProVal Thr Leu 195 200 205 Val Leu Gly Glu Asn Asp Asn Glu Val Gly Tyr GlnAsp Gln Val Val 210 215 220 Phe Ala His Lys Gly Ala His Met Thr Thr ThrVal Ile Pro Asn Ala 225 230 235 240 Gly His Asn Leu Met Ile Asp Ala ProGlu Ala Val Met Thr Ala Phe 245 250 255 His Gln Phe Leu His Lys 260 72309 PRT Lactobacillus rhamnosus 72 Met Val Thr Ala Ala Asp Asn Ile ThrGly Leu Ile Gly Asn Thr Pro 1 5 10 15 Leu Leu Lys Leu Asn Arg Val ValPro Glu Gly Ala Ala Asp Val Tyr 20 25 30 Val Lys Leu Glu Phe Phe Asn ProGly Gly Ser Val Lys Asp Arg Ile 35 40 45 Ala Leu Ala Met Ile Glu Asp AlaGlu Tyr Lys Gly Val Leu Lys Pro 50 55 60 Gly Gly Thr Ile Val Glu Pro ThrSer Gly Asn Thr Gly Ile Gly Leu 65 70 75 80 Ala Leu Val Ala Ala Ala LysGly Tyr His Leu Ile Ile Thr Met Pro 85 90 95 Glu Thr Met Ser Val Glu ArgArg Ala Leu Met Arg Gly Tyr Gly Ala 100 105 110 Glu Leu Ile Leu Thr ProGly Ala Asp Gly Met Pro Gly Ala Ile Lys 115 120 125 Lys Ala Glu Ala LeuSer Lys Glu Asn Gly Tyr Phe Leu Pro Met Gln 130 135 140 Phe Gln Asn ProAla Asn Pro Asp Val His Glu Arg Thr Thr Gly Gln 145 150 155 160 Glu IleIle Arg Ser Phe Asp Gly Gly Thr Pro Asp Ala Phe Val Ala 165 170 175 GlyVal Gly Thr Gly Gly Thr Leu Thr Gly Val Gly Arg Ala Leu Arg 180 185 190Lys Ile Asn Pro Asp Val Gln Ile Tyr Ala Leu Glu Ala Ala Glu Ser 195 200205 Pro Met Leu Lys Glu Gly His Gly Gly Lys His Lys Ile Gln Gly Ile 210215 220 Ser Ala Gly Phe Ile Pro Asp Val Leu Asp Thr Asn Leu Tyr Gln Asp225 230 235 240 Ile Ile Glu Val Thr Ser Asp Gln Ala Ile Asp Met Ala ArgHis Val 245 250 255 Ser His Glu Glu Gly Phe Leu Pro Gly Ile Ser Ala GlyAla Asn Ile 260 265 270 Phe Gly Ala Ile Glu Ile Ala Lys Lys Leu Gly LysGly Lys Ser Val 275 280 285 Ala Thr Val Ala Pro Asp Asn Gly Glu Arg TyrLeu Ser Thr Asp Leu 290 295 300 Phe Lys Phe Asp Asp 305 73 270 PRTLactobacillus rhamnosus 73 Met Leu Lys Lys Lys Leu Trp Phe Leu Leu ProLeu Val Ala Leu Val 1 5 10 15 Thr Phe Thr Leu Thr Ala Cys Thr Ser AlaSer Ser Asp Thr Ser Lys 20 25 30 Asn Ser Asp Val Thr Ala Glu Leu Ile AsnLys Asn Glu Leu Thr Ile 35 40 45 Gly Leu Glu Gly Thr Tyr Ala Pro Phe SerTyr Arg Lys Asp Gly Lys 50 55 60 Leu Glu Gly Phe Glu Val Glu Leu Gly LysAla Leu Ala Lys Lys Ile 65 70 75 80 Gly Val Lys Ala Lys Phe Val Pro ThrGln Trp Asp Ser Leu Ile Ala 85 90 95 Gly Leu Gly Ser Gln Lys Phe Asp LeuVal Leu Asn Asp Ile Ser Glu 100 105 110 Thr Pro Ala Arg Lys Lys Val TyrAsn Phe Thr Thr Pro Tyr Met Tyr 115 120 125 Ser Arg Tyr Ala Leu Ile ThrArg Ser Asp Asn Thr Thr Ile Lys Ser 130 135 140 Leu Ala Asp Ile Lys GlyLys Thr Phe Val Glu Gly Thr Gly Thr Pro 145 150 155 160 Asn Ala Ala LeuAla Lys Lys Tyr Gly Ala Lys Ile Thr Pro Ser Gly 165 170 175 Asp Phe ThrVal Ser Leu Ser Leu Val Lys Glu Lys Arg Ala Asp Gly 180 185 190 Thr IleAsn Ala Ser Ala Ala Trp Tyr Ala Phe Ala Lys Asn Asn Ser 195 200 205 ThrAla Gly Leu Lys Ser Gln Thr Leu Lys Asp Ser Val Val Lys Pro 210 215 220Asp Glu Val Ala Gly Met Val Ser Lys Lys Ser Pro Lys Leu Gln Ala 225 230235 240 Ala Leu Ser Lys Gly Ile Gln Glu Leu Arg Lys Asp Gly Thr Leu Lys245 250 255 Lys Leu Ser Gln Lys Tyr Phe Gly Thr Asp Leu Thr Thr Lys 260265 270 74 474 PRT Lactobacillus rhamnosus 74 Ile Cys Lys Gly Arg SerLeu Lys Pro Phe Gly His Phe Ile Asp Ala 1 5 10 15 Ile Thr Val Asn ArgGlu His Val Leu Thr Thr Ala Ala Glu Ala Leu 20 25 30 Ile Ala Ser Ala GlyAsp Ala Leu Asn Ala Ser His Ala Thr Phe Asn 35 40 45 Val Leu Asn Asn SerAsp Leu Gln Phe Gly Phe Val Glu Asn Glu Asp 50 55 60 Gly Glu Thr Val GlnLeu Ser Asn Gly Leu Tyr Gly Gln Leu Ile Arg 65 70 75 80 Ser Thr Asn ArgLys Leu Arg Lys Glu Ala Phe Glu Ala Leu Leu Arg 85 90 95 Ala Tyr Glu SerLeu Lys Asn Thr Phe Ala Gln Thr Leu Ser Gly Gln 100 105 110 Val Lys AlaHis Asn Phe Asn Ala Thr Ala His His Tyr Lys Asn Ala 115 120 125 Arg AlaAla Ala Met Ala Ser Asn His Ile Pro Glu Ser Val Tyr Thr 130 135 140 ThrLeu Ile Asp Gln Val Asn Thr His Leu Pro Leu Leu His Arg Tyr 145 150 155160 Val Ala Leu Arg Lys Lys Val Leu Ala Val Asp Gln Leu His Met Tyr 165170 175 Asp Ile Tyr Thr Pro Leu Thr Gly Gln Pro Pro Leu Thr Tyr Thr Leu180 185 190 Glu Gln Ala Lys Ala Glu Ala Leu Lys Ala Leu Ala Pro Leu GlyAsp 195 200 205 Asp Tyr Leu Glu His Val Arg Glu Ile Phe Asp Asn Arg TyrIle Asp 210 215 220 Val Val Glu Asn Lys Gly Lys Arg Ser Gly Ala Tyr SerGly Gly Ala 225 230 235 240 Tyr Asp Thr Asn Pro Phe Ile Leu Leu Asn TrpHis Asp Ala Val Asp 245 250 255 Glu Leu Tyr Thr Leu Val His Glu Thr GlyHis Ser Val His Ser Trp 260 265 270 Tyr Thr Arg His Asn Gln Pro Tyr ValTyr Gly Asp Tyr Pro Ile Phe 275 280 285 Val Ala Glu Ile Ala Ser Thr ThrAsn Glu Asn Leu Leu Thr Asp Tyr 290 295 300 Phe Leu Thr His Ser Asp AspPro Lys Val Arg Ala Tyr Ile Leu Asn 305 310 315 320 Tyr Tyr Leu Asp GlyPhe Lys Gly Thr Val Phe Arg Gln Thr Gln Phe 325 330 335 Ala Glu Phe GluHis Trp Ile His Gln Gln Asp Gln Gln Gly Glu Pro 340 345 350 Leu Thr AlaThr Ser Met Ser Gln Tyr Tyr Ala Asp Leu Asn Ala Arg 355 360 365 Tyr TyrGly Pro Glu Val Ala Arg Asp Pro Glu Ile Ala Phe Glu Trp 370 375 380 AlaArg Ile Pro His Phe Tyr Tyr Asn Tyr Tyr Val Tyr Gln Tyr Ala 385 390 395400 Thr Gly Phe Ala Ala Ala Ser Thr Leu Ala Ala Gly Ile Ser Ser Gly 405410 415 Glu Pro Asp Ala Ala Ala His Tyr Leu Asp Tyr Leu Lys Ser Gly Ser420 425 430 Ser Lys Tyr Ala Ile Asp Thr Met Lys Thr Ala Gly Val Asp MetThr 435 440 445 Lys Pro Asp Tyr Leu Glu Ala Ala Phe Ser Val Phe Glu GlnArg Leu 450 455 460 Thr Glu Leu Glu Lys Ile Leu Gln Lys Gly 465 470 75256 PRT Lactobacillus rhamnosus 75 Ser Tyr Ala Pro Thr Ile Thr Leu GluGln Ala Lys Glu Asp Ile Lys 1 5 10 15 Asn Ala Thr Ala Leu Met Gly GlnAsp Tyr Gln Ala Gln Met Met Gln 20 25 30 Ala Phe Ser Glu Arg Trp Ile AspPhe Pro Ala Asn Gln Gly Lys Asp 35 40 45 Ser Gly Ala Tyr Thr Ala Gly ProTyr Gly Val His Pro Tyr Val Glu 50 55 60 Met Thr Trp Ser Asn Thr Leu ProAla Val Tyr Thr Leu Ile His Glu 65 70 75 80 Leu Gly His Thr Ala Gln MetVal Arg Ser Gln Glu Ala His Asn Val 85 90 95 Leu Asp Ala Asp Phe Asn AlaTyr Leu Val Glu Ser Pro Ser Thr Phe 100 105 110 Asn Glu Leu Leu Leu ThrHis Tyr Leu Glu Glu Asn Ala Lys Asp Pro 115 120 125 Arg Met Lys Arg PheAla Leu Ser Arg Leu Leu Asn Asp Thr Tyr Phe 130 135 140 His Asn Phe ValThr His Leu Leu Glu Ala Ala Phe Gln Arg Glu Val 145 150 155 160 Tyr AsnLeu Ile Asp Asn Gly Glu Thr Phe Asp Ala Ala Arg Leu Asn 165 170 175 AlaIle Thr Arg Lys Val Leu Thr Asp Phe Trp Gly Ser Ala Val Glu 180 185 190Leu Glu Pro Gly Ala Glu Leu Thr Trp Met Arg Gln Ser His Tyr Tyr 195 200205 Met Gly Leu Tyr Ser Tyr Ser Tyr Ser Ala Gly Leu Thr Val Ala Thr 210215 220 Gln Ala Phe Gln Ala Ile Glu Gln Gln Gly Gln Pro Ala Val Asp Arg225 230 235 240 Trp Leu Arg Tyr Leu Ser Leu Gly Asp Ser Leu Asp Pro ValGlu Ala 245 250 255 76 641 PRT Lactobacillus rhamnosus 76 Leu Leu GlyGln Phe Gly Val Asp Leu Thr Glu Gln Ala Arg Lys Gly 1 5 10 15 Gln IleAsp Pro Val Ile Gly Arg Asp Lys Glu Ile Ser Arg Val Ile 20 25 30 Glu IleLeu Asn Arg Arg Thr Lys Asn Asn Pro Val Leu Ile Gly Glu 35 40 45 Ala GlyVal Gly Lys Thr Ala Val Val Glu Gly Leu Ala Leu Lys Ile 50 55 60 Ala AsnGly Asp Val Pro Ala Lys Leu Gln Asp Arg His Val Ile Arg 65 70 75 80 LeuAsp Val Val Ser Leu Val Gln Gly Thr Gly Ile Arg Gly Gln Phe 85 90 95 GluGln Arg Met Gln Gln Leu Ile Asp Glu Leu Lys Gln Asn Lys Asn 100 105 110Ile Ile Leu Phe Ile Asp Glu Ile His Glu Ile Val Gly Ala Gly Asn 115 120125 Ala Glu Gly Gly Met Asp Ala Gly Asn Val Leu Lys Pro Ala Leu Ala 130135 140 Arg Gly Glu Leu Gln Leu Val Gly Ala Thr Thr Ser Asn Glu Tyr Arg145 150 155 160 Gln Ile Glu Lys Asp Ser Ala Leu Ala Arg Arg Leu Gln ProVal Met 165 170 175 Val Glu Glu Pro Ser Val Asp Glu Thr Ile Lys Ile LeuLys Gly Leu 180 185 190 Gln Pro Arg Tyr Gln Asp Phe His His Val Lys TyrThr Glu Gly Ala 195 200 205 Ile Glu Ala Ala Ala Thr Leu Ser Asn Arg TyrIle Gln Asp Arg Phe 210 215 220 Leu Pro Asp Lys Ala Ile Asp Leu Leu AspGlu Ala Gly Ser Arg Lys 225 230 235 240 Asn Leu Thr Ile Ala Thr Val AspPro Glu Thr Ile Lys Ala Lys Ile 245 250 255 Ala Asp Ala Glu Lys Gln LysGln Ala Ala Leu Lys Gln Glu Asp Tyr 260 265 270 Glu Lys Ala Ala Phe TyrArg Asp Gln Val Thr Lys Leu Glu Asp Met 275 280 285 Ala Lys Lys Gln SerAsn Leu Pro Asp Asn Glu Ile Pro Thr Val Thr 290 295 300 Glu Lys Asp MetGlu Lys Ile Val Glu Glu Lys Thr Asn Ile Pro Val 305 310 315 320 Gly GluLeu Lys Ala Gln Glu Gln Ala Gln Leu Lys Asn Leu Ala Ser 325 330 335 AspLeu Glu Gln His Val Ile Gly Gln Asn Glu Ala Val Asp Lys Val 340 345 350Ala Arg Ala Ile Arg Arg Asn Arg Ile Gly Phe Asn Lys Thr Gly Arg 355 360365 Pro Ile Gly Ser Phe Leu Phe Val Gly Pro Thr Gly Val Gly Lys Thr 370375 380 Glu Leu Ala Lys Gln Leu Ala Lys Glu Leu Phe Gly Ser Glu Asp Ala385 390 395 400 Met Ile Arg Phe Asp Met Ser Glu Tyr Met Glu Lys Phe SerVal Ser 405 410 415 Lys Leu Ile Gly Ser Pro Pro Gly Tyr Val Gly Tyr GluGlu Ala Gly 420 425 430 Gln Leu Thr Glu Lys Val Arg Arg Asn Pro Tyr SerLeu Ile Leu Leu 435 440 445 Asp Glu Ile Glu Lys Ala His Pro Asp Val MetAsn Met Phe Leu Gln 450 455 460 Ile Leu Asp Asp Gly Arg Leu Thr Asp SerGln Gly Arg Thr Val Ser 465 470 475 480 Phe Lys Asp Thr Ile Ile Ile MetThr Ser Asn Ala Gly Ser Thr Asp 485 490 495 Ala Glu Ala Asn Val Gly PheGly Ala Thr Leu Ser Gly Lys Thr His 500 505 510 Ser Val Leu Asp Gln LeuGly Asn Tyr Phe Lys Pro Glu Phe Leu Asn 515 520 525 Arg Phe Asp Asp IleVal Glu Phe Lys Pro Leu Ser Lys Asp Asp Leu 530 535 540 Leu Lys Ile ValSer Leu Met Ile Asn Asp Thr Asn Asn Asn Leu Lys 545 550 555 560 Ser GlnGly Leu Thr Ile His Val Thr Asp Pro Val Lys Glu Lys Leu 565 570 575 ValThr Leu Gly Tyr Asn Pro Ser Met Gly Ala Arg Pro Leu Arg Arg 580 585 590Val Ile Gln Glu Gln Ile Glu Asp Arg Val Ala Asp Phe Tyr Leu Asp 595 600605 His Pro Asn Ala Lys Glu Leu Glu Ala Arg Ile Ser Asn Gly Glu Ile 610615 620 Thr Val Gly Glu Pro Ala Lys Ala Glu Ala Ser Ser Lys Thr Ala Lys625 630 635 640 Lys 77 481 PRT Lactobacillus rhamnosus 77 Thr Lys SerVal Val Gly Val Ala Pro Glu Ser Gln Leu Leu Ala Met 1 5 10 15 Lys ValPhe Thr Asn Ser Asp Thr Ser Ala Thr Thr Gly Ser Ser Thr 20 25 30 Leu ValSer Ala Ile Glu Asp Ser Ala Lys Leu Gly Ala Asp Val Leu 35 40 45 Asn MetSer Leu Gly Ser Val Ser Gly Asn Gln Thr Leu Glu Asp Pro 50 55 60 Glu IleAla Ala Val Gln Asn Ala Asn Glu Ser Gly Thr Ala Ala Val 65 70 75 80 IleSer Ala Gly Asn Ser Gly Thr Ser Gly Ser Gly Thr Glu Gly Val 85 90 95 AsnLys Asp Tyr Tyr Gly Leu Gln Asp Asn Glu Thr Val Gly Thr Pro 100 105 110Gly Thr Ser Arg Gly Ala Thr Thr Val Ala Ser Ala Glu Asn Thr Asp 115 120125 Val Ile Asn Gln Ala Val Thr Ile Thr Asp Gly Ser Gly Leu Lys Leu 130135 140 Gly Pro Glu Thr Val Gln Leu Ser Ser Asn Asp Phe Val Asp Ser Phe145 150 155 160 Asp Gln Lys Lys Phe Tyr Val Val Lys Asp Ala Ser Gly LysLeu Ser 165 170 175 Thr Gly Asp Ala Gly Asp Tyr Thr Ala Asp Ala Lys GlyLys Ile Ala 180 185 190 Ile Val Lys Arg Gly Ser Leu Thr Phe Thr Asp LysGln Lys Tyr Ala 195 200 205 Glu Ala Ala Gly Ala Ala Gly Leu Ile Ile ValAsn Asn Asp Gly Thr 210 215 220 Ser Thr Pro Leu Thr Ser Ile Ser Leu ThrAla Thr Phe Pro Thr Phe 225 230 235 240 Gly Leu Ser Asn Thr Thr Gly GlnLys Leu Val Asp Trp Val Thr Ala 245 250 255 His Pro Asn Asp Ser Leu GlyVal Lys Ile Ala Leu Ala Leu Leu Pro 260 265 270 Asn Gln Asn Tyr Lys AlaAsp Arg Met Ser Ser Phe Thr Ser Tyr Gly 275 280 285 Pro Val Ser Asp LeuSer Phe Lys Pro Asp Ile Thr Ala Pro Gly Gly 290 295 300 Asn Ile Trp SerThr Gln Asn Asn Asn Gly Tyr Thr Asn Met Ser Gly 305 310 315 320 Thr SerMet Ala Ser Pro Phe Ile Ala Gly Ser Gln Ala Leu Leu Lys 325 330 335 GlnAla Leu Asn Asn Lys Asp Asn Glu Phe Tyr Ala Asp Tyr Lys Gln 340 345 350Leu Lys Gly Thr Ala Leu Thr Asp Phe Leu Lys Thr Val Glu Met Asn 355 360365 Thr Ala Lys Pro Ile Asn Asp Ile Asn Tyr Asp Asn Val Ile Val Ser 370375 380 Pro Arg Arg Gln Gly Ala Gly Leu Val Asp Val Lys Ala Ala Ile Asp385 390 395 400 Ala Leu Glu Lys Asn Pro Ser Thr Val Val Ser Glu Asn GlyTyr Pro 405 410 415 Ala Val Glu Leu Lys Asp Phe Thr Ser Thr Thr Lys ThrPhe Lys Leu 420 425 430 Thr Phe Thr Asn Arg Thr Lys His Gln Leu Thr TyrGln Met Thr Ser 435 440 445 Asn Glu Asp Thr Asn Ala Val Tyr Thr Ser AlaThr Asp Leu Glu Ser 450 455 460 Phe Ile Gln Ser Ser Lys Met Ala Lys LeuIle His Glu Arg Gly Ala 465 470 475 480 Ala 78 331 PRT Lactobacillusrhamnosus 78 Met Thr Ile Asn Trp Gln Gln Glu Val Glu Lys Leu Glu Pro GlnLeu 1 5 10 15 Leu Ser Asp Leu Thr Thr Leu Leu Lys Ile Asn Ser Glu ArgAsp Thr 20 25 30 Asp His Gln Thr Asp Glu Tyr Pro Leu Gly Pro Gly Pro AlaLys Ala 35 40 45 Leu Glu Ala Phe Leu Ala Ile Ala Gln Arg Asp Gly Phe LysThr Leu 50 55 60 Asn Val Asp His Val Ala Gly Arg Ile Glu Leu Gly Asp GlyAsp Glu 65 70 75 80 Ile Phe Gly Leu Phe Gly His Val Asp Val Val Pro AlaGly Pro Gly 85 90 95 Trp Gln Thr Asp Pro Phe Asp Pro Val Ile Arg Asp GlyLys Ile Tyr 100 105 110 Gly Arg Gly Thr Ser Asp Asp Lys Gly Pro Ser IleAla Ala Tyr Tyr 115 120 125 Ala Leu Lys Leu Ile Arg Asp Leu Lys Leu ProIle Asn Lys Lys Ile 130 135 140 His Phe Ile Leu Gly Thr Asp Glu Glu SerAsp Trp Val Gly Ile His 145 150 155 160 Arg Tyr Leu Glu Thr Glu Pro AlaPro Asp Phe Gly Phe Ser Pro Asp 165 170 175 Ala Glu Phe Pro Ile Ile AsnGly Glu Lys Gly Ile Ala Ser Phe Glu 180 185 190 Ile Val Gln Lys Pro IleAla Ala Ala Thr Ala Asp Leu Thr Leu Asn 195 200 205 His Phe Ser Ala GlyIle Arg Pro Asn Met Val Pro Gln Glu Ala Lys 210 215 220 Ala Val Leu SerGly Pro Leu Pro Glu Ala Phe Val Thr Gln Ala Glu 225 230 235 240 Lys TrpAla Ala Glu Gln Glu Val Thr Leu Thr Leu Thr Leu Gly Asn 245 250 255 ProThr Thr Ile Glu Leu Ile Gly Lys Gly Ala His Ala Gln Glu Pro 260 265 270Lys Asp Gly Lys Asn Ala Ala Thr Tyr Leu Ala Thr Leu Leu Ala Asp 275 280285 Leu Pro Phe Asp Pro Ala Gly Lys Ala Tyr Leu Thr Met Ile Ala Asn 290295 300 His Leu His Leu Asp Ser Arg Gly His His Leu Gly Ile Asn Tyr Thr305 310 315 320 Asp Lys Leu Met Gly Asp Leu Thr Ala Ser Pro 325 330 79344 PRT Lactobacillus rhamnosus VARIANT (1)...(344) Xaa = Any Amino Acid79 Gly Lys Met Ser Leu Tyr Ala Gly Gly Pro Asp Glu Arg Leu Thr Pro 1 510 15 Leu Ile Asp Gly Arg Arg His Val Thr Asp Phe Ala Leu Thr Pro Asp 2025 30 His Arg Gly Val Val Phe Thr Glu Ser Thr Met Thr Ile Pro Ser Arg 3540 45 Leu Val Tyr Phe Asp Leu Ala Ser Glu Glu Glu Gln Val Leu Tyr Asp 5055 60 Pro Asn Arg Gln Val Thr Arg His Leu Gly Leu Val Thr Pro Gln Thr 6570 75 80 Phe Asn Phe Gln Arg Asp Gly Phe Glu Ile Glu Gly Trp Tyr Phe Pro85 90 95 Pro Gln Gln Ala Ser Ser Ser His Pro Ala Ile Leu Tyr Val His Gly100 105 110 Gly Pro Ala Val Gly Tyr Gly Tyr Thr Phe Phe His Glu Met GlnTyr 115 120 125 Leu Ala Ala Lys Gly Tyr Gly Val Ile Cys Arg Asn Pro ArgGly Gly 130 135 140 Leu Gly Tyr Arg Glu Ala Phe Thr Gly Ala Val Ile LysHis Xaa Pro 145 150 155 160 Ala Gly Asp Tyr Glu Asp Cys Leu Ala Ser GlyGlu Glu Ala Leu Lys 165 170 175 Leu Asp Thr Thr Ile Asp Pro Gln Arg LeuPhe Val Thr Gly Gly Ser 180 185 190 Tyr Gly Gly Phe Met Thr Asn Trp IleVal Thr His Thr His Arg Phe 195 200 205 Lys Ala Ala Val Thr Gln Arg SerIle Ser Asn Trp Leu Ser Met Tyr 210 215 220 Gly Thr Ser Asp Ile Gly TyrTyr Phe Thr Pro Trp Glu Leu Glu Gly 225 230 235 240 Lys Trp Thr Gly AspLeu Ser Asp Val Gln Gly Leu Trp Asp Phe Ser 245 250 255 Pro Leu Ala HisIle Asp His Ala Arg Thr Pro Thr Leu Val Met His 260 265 270 Ser Glu AsnAsp Glu Arg Cys Pro Ile Gly Pro Ser Arg Lys Val Asp 275 280 285 His ArgSer Gln Thr Ala Trp Cys Xaa Asn Gln Val His Ala Phe Pro 290 295 300 LysVal Lys Ser Xaa Phe Val Pro Ala Ala Gly Leu Pro Asn Leu Arg 305 310 315320 Val Ala Arg Leu Gln Ala Ile Val Asp Trp Phe Asp Ala His Gln Ala 325330 335 Gln Pro Gln Met Ala Lys Gly Glu 340 80 558 PRT Lactobacillusrhamnosus 80 His Leu Ile Gly Ala Thr Thr Leu Asp Glu Tyr Arg Glu Asn IleGlu 1 5 10 15 Lys Asp Lys Ala Leu Glu Arg Arg Phe Gln Arg Val Leu ValGln Glu 20 25 30 Pro Thr Val Glu Asp Thr Ile Ser Ile Leu Arg Gly Leu LysGlu Arg 35 40 45 Phe Glu Ile Phe His Lys Val Arg Ile His Asp Ser Ala LeuVal Ala 50 55 60 Ala Ala Thr Leu Ser Asn Arg Tyr Ile Thr Asp Arg Phe LeuPro Asp 65 70 75 80 Lys Ala Ile Asp Leu Val Asp Glu Ala Cys Ala Thr IleAsn Val Glu 85 90 95 Met Asn Ser Arg Pro Thr Glu Leu Asp Val Ala Glu ArgLys Gln Met 100 105 110 Gln Leu Glu Ile Glu Gln Gln Ala Leu Lys Asn GluSer Asp Pro Ala 115 120 125 Ser Lys Lys Arg Leu Glu Asn Ala Asn Ala GluLeu Ala Asn Leu Lys 130 135 140 Glu Lys Thr Asn Lys Leu Lys Ala Gln TrpGlu Ala Glu Lys Lys Asp 145 150 155 160 Ile Arg Gln Leu Asn Glu Lys LysSer Ala Ile Asp Lys Ala Lys His 165 170 175 Glu Leu Glu Asp Ala Gln SerArg Tyr Asp Leu Glu Thr Ala Ala Arg 180 185 190 Leu Gln His Gly Thr IlePro Gln Leu Glu Lys Glu Leu Gln Ala Met 195 200 205 Glu His Ser Asp ArgPro Gln Ser Trp Leu Val Gln Glu Ser Val Thr 210 215 220 Ala Asn Glu IleAla Ala Val Ile Ser Arg Glu Thr Gly Ile Pro Val 225 230 235 240 Ala LysLeu Val Glu Gly Asp Arg Gln Lys Leu Leu His Leu Ala Gly 245 250 255 AsnLeu His Gln Arg Val Ile Gly Gln Asp Glu Ala Val Thr Ala Val 260 265 270Ser Asp Ala Val Leu Arg Ser Arg Ala Gly Leu Gln Asp Pro Ser Arg 275 280285 Pro Leu Gly Ser Phe Leu Phe Leu Gly Pro Thr Gly Val Gly Lys Thr 290295 300 Glu Leu Ala Lys Ala Leu Ala Glu Asp Leu Phe Asp Ser Glu Lys His305 310 315 320 Met Val Arg Ile Asp Met Ser Glu Tyr Met Glu Lys Ala SerVal Ser 325 330 335 Arg Leu Val Gly Ala Ala Pro Gly Tyr Val Gly Tyr GluGln Gly Gly 340 345 350 Gln Leu Thr Glu Ala Val Arg Arg Asn Pro Tyr ThrIle Val Leu Leu 355 360 365 Asp Glu Ile Glu Lys Ala Asn Pro Asp Val PheAsn Ile Leu Leu Gln 370 375 380 Val Leu Asp Asp Gly Arg Leu Thr Asp GlyGln Gly Arg Thr Val Asp 385 390 395 400 Phe Lys Asn Thr Ile Ile Ile MetThr Ser Asn Leu Gly Ser Glu Tyr 405 410 415 Leu Leu Asp Gly Val Gln LysAsp Gly Thr Val Ser Gln Gln Ala Lys 420 425 430 Asp Gln Val Arg Gln LeuIle Gly Lys Ala Phe Lys Pro Glu Phe Leu 435 440 445 Asn Arg Ile Asp AspIle Ile Met Phe His Pro Leu Ser Leu Asp Asp 450 455 460 Val Lys Lys IleAla Val Lys Asp Leu His Glu Leu Gly Thr Arg Leu 465 470 475 480 Ala AspGln Gln Ile Ser Leu Asp Ile Thr Pro Glu Ala Gln Thr Trp 485 490 495 LeuAla Asp Lys Gly Tyr Asp Pro Ala Phe Gly Ala Arg Pro Leu Gln 500 505 510Arg Leu Ile Thr Ser Ala Val Glu Thr Pro Leu Ala Lys Glu Leu Ile 515 520525 Arg Gly Thr Ile Gln Pro Gly Gln Glu Val Val Ile Thr Val Ala Asp 530535 540 Asp Gln Leu Gln Phe Lys Ala Lys Gln Val Val Ala Lys Ala 545 550555 81 292 PRT Lactobacillus rhamnosus 81 Ile Ser Ala Ile Ile Val IleVal Glu Glu Asn Asn Val Ala Ala Arg 1 5 10 15 Glu Leu Ile Leu Ala PheGlu Ser Ser Cys Asp Glu Thr Ser Val Ala 20 25 30 Val Val Glu Asn Gly ThrLys Ile Leu Ser Asn Ile Ile Ala Thr Gln 35 40 45 Ile Lys Ser His Gln ArgPhe Gly Gly Val Val Pro Glu Val Ala Ser 50 55 60 Arg His His Val Glu GlnIle Thr Leu Val Thr Asp Ala Ala Leu Lys 65 70 75 80 Glu Ala Gly Val ThrTyr Thr Asp Leu Thr Ala Val Ala Val Thr Tyr 85 90 95 Gly Pro Gly Leu ValGly Ala Leu Leu Ile Gly Val Arg Ala Ala Lys 100 105 110 Pro Ile Ala TyrAla His His Leu Pro Leu Ile Pro Val Asn His Met 115 120 125 Ala Gly HisIle Tyr Ala Ala Arg Phe Val Lys Pro Leu Val Tyr Pro 130 135 140 Leu LeuAla Leu Ala Val Ser Gly Gly His Thr Glu Leu Val Tyr Met 145 150 155 160Arg Ala Ala Gly Glu Phe Glu Ile Ile Gly Asp Thr Arg Asp Asp Ala 165 170175 Ala Gly Glu Ala Tyr Asp Lys Val Gly Arg Ile Leu Gly Ile Pro Tyr 180185 190 Pro Ala Gly Lys Glu Val Asp Arg Leu Ala His Leu Gly His Asp Thr195 200 205 Phe His Phe Pro Arg Ala Met Asp Lys Glu Asp Asn Leu Asp PheSer 210 215 220 Phe Ser Gly Leu Lys Ser Ala Val Ile Asn Thr Val His HisAla Asp 225 230 235 240 Gln Ile Gly Glu Ser Leu Ser Arg Glu Asp Leu SerAla Ser Ser Gln 245 250 255 Ala Ser Val Val His Val Met Val Leu Lys SerGln Ser Ala Ile Ala 260 265 270 Glu Tyr Pro Val Ile Gln Val Val Ile AlaGly Gly Val Ala Asp Asn 275 280 285 Gln Gly Leu Lys 290 82 244 PRTLactobacillus rhamnosus 82 Met Ile Phe Arg Lys Pro Gln Pro Phe Glu TyrGlu Gly Thr Asp Thr 1 5 10 15 Gly Val Val Leu Leu His Ala Tyr Thr GlySer Pro Asn Asp Met Asn 20 25 30 Phe Met Ala Arg Ala Leu Gln Arg Ser GlyTyr Gly Val Tyr Val Pro 35 40 45 Leu Phe Ser Gly His Gly Thr Val Glu ProLeu Asp Ile Leu Thr Lys 50 55 60 Gly Asn Pro Asp Ile Trp Trp Ala Glu SerSer Ala Ala Val Ala His 65 70 75 80 Met Thr Ala Lys Tyr Ala Lys Val PheVal Phe Gly Leu Ser Leu Gly 85 90 95 Gly Ile Phe Ala Met Lys Ala Leu GluThr Leu Pro Gly Ile Thr Ala 100 105 110 Gly Gly Val Phe Ser Ser Pro IleLeu Pro Gly Lys His His Leu Val 115 120 125 Pro Gly Phe Leu Lys Tyr AlaGlu Tyr Met Asn Arg Leu Ala Gly Lys 130 135 140 Ser Asp Glu Ser Thr GlnIle Leu Ala Tyr Leu Pro Gly Gln Leu Ala 145 150 155 160 Ala Ile Asp GlnPhe Ala Thr Thr Val Ala Ala Asp Leu Asn Leu Val 165 170 175 Lys Gln ProThr Phe Ile Gly Gln Ala Gly Gln Asp Glu Leu Val Asp 180 185 190 Gly ArgLeu Ala Tyr Gln Leu Arg Asp Ala Leu Ile Asn Ala Ala Arg 195 200 205 ValAsp Phe His Trp Tyr Asp Asp Ala Lys His Val Ile Thr Val Asn 210 215 220Ser Ala His His Ala Leu Glu Glu Asp Val Ile Ala Phe Met Gln Gln 225 230235 240 Glu Asn Glu Gly 83 433 PRT Lactobacillus rhamnosus 83 Leu GlyIle Phe Phe Phe Lys Arg Phe Arg Lys Leu His Leu Phe Asp 1 5 10 15 ProLeu Asn Tyr Pro Glu Glu Thr Phe Gln Ser Phe Asp Ser Ala Phe 20 25 30 AsnAsn Gly Ala Asp Tyr Val Glu Leu Asp Val His Glu Ser Ala Asp 35 40 45 GlyVal Ile Val Ile Gln His Asp Thr Thr Ile Gln Arg Thr Thr Gly 50 55 60 AlaAsn Leu Ala Ile Ala Lys Thr Asn Phe Ala Gln Leu Gln Gln Tyr 65 70 75 80His Thr Lys Asn Gly Glu Pro Ile His Ser Leu Glu Glu Leu Phe Ala 85 90 95His Glu Gln Gln Thr Lys His Lys Phe Leu Ile Glu Thr Lys Ile Val 100 105110 Lys Gly Glu Pro His Pro His Leu Glu Asp Lys Val Ala Ala Leu Ile 115120 125 Lys Gln Tyr His Met Glu Asn Arg Val Met Phe His Ser Phe Ser Ala130 135 140 Ala Ser Leu Lys Arg Leu Gln Ala Ala Leu Pro Asn Ile Pro ArgIle 145 150 155 160 Leu Ile Val Gly Ser Leu Lys Arg Ile Asn Phe Asp ValLeu Thr Tyr 165 170 175 Val Asp Gly Ile Asn Leu Ser Ser Asp Leu Val ThrPro Gln Leu Val 180 185 190 Thr Gln Leu His Asp Leu Gly Lys Lys Val TyrVal Trp Asp Glu Met 195 200 205 Asn Glu Asp Arg Ala Lys Trp Thr Trp LeuVal Asn Leu Asn Ile Asp 210 215 220 Gly Val Val Thr Asn Tyr Thr Ser LeuGly His Glu Phe Gln Thr Leu 225 230 235 240 Lys Ala Ala Ala Val Thr ThrSer Ile Asn Asp Leu Gly Ala Asn Ser 245 250 255 Ser Leu Ala Ala Leu ProVal Tyr Glu Asn Pro Tyr Gln Pro Leu Leu 260 265 270 Arg Ser Glu Arg LeuAla Pro Gln Thr Pro Ile Met Ile Ser Ser Met 275 280 285 Val Ser Leu AlaGly Ser Thr Tyr Tyr Gln Ile Gly Asp Asn Ala Phe 290 295 300 Val Pro AlaGlu Thr Ile Asn Leu Ala Pro Glu Ala Gly Trp Ala Ser 305 310 315 320 LeuPhe Leu His Gln Arg Ile Val Ile Thr Ser Arg His Phe Lys Val 325 330 335Pro Val His Ala Asp Pro Leu His Gln Gln Ala Ile Thr Gly His Val 340 345350 Gly Asn His Lys Cys Tyr Arg Val Leu Ala Ala Arg Tyr Gln Ser Gly 355360 365 Gln Leu Tyr Leu Lys Thr Lys Ile Gly Trp Leu Asn Ala Lys Asp Leu370 375 380 Gln Val Leu Pro Thr Ala Glu Asn Met Arg Ile Trp Leu Thr LeuTyr 385 390 395 400 Arg Ser Ile Pro Glu Asn Gln Lys Pro Leu Leu His TrpAla Leu Gly 405 410 415 Asp Thr Ala Phe Asp Thr Pro Leu Leu Asn Ala SerVal Leu Asn Ile 420 425 430 Gly 84 448 PRT Lactobacillus rhamnosus 84Met Glu Leu Ala Lys Leu Ala Val Asp Glu Thr Gly Arg Gly Val Trp 1 5 1015 Glu Asp Lys Ala Ile Lys Asn Met Phe Ala Thr Glu Glu Ile Trp His 20 2530 Ser Ile Lys Asn Asn Lys Thr Val Gly Val Ile Asn Glu Asp Lys Gln 35 4045 Arg Gly Leu Val Ser Ile Ala Glu Pro Ile Gly Val Ile Ala Gly Val 50 5560 Thr Pro Val Thr Asn Pro Thr Ser Thr Thr Ile Phe Lys Ser Glu Ile 65 7075 80 Ser Ile Lys Thr Arg Asn Pro Ile Ile Phe Ala Phe His Pro Gly Ala 8590 95 Gln Lys Ser Ser Ala Arg Ala Leu Glu Val Ile Arg Glu Glu Ala Glu100 105 110 Lys Ala Gly Leu Pro Lys Gly Ala Leu Gln Tyr Ile Pro Val ProSer 115 120 125 Met Glu Ala Thr Lys Thr Leu Met Asp His Pro Gly Ile AlaThr Ile 130 135 140 Leu Ala Thr Gly Gly Pro Gly Met Val Lys Ser Ala TyrSer Ser Gly 145 150 155 160 Lys Pro Ala Leu Gly Val Gly Ala Gly Asn AlaPro Ala Tyr Ile Glu 165 170 175 Ala Ser Ala Asn Ile Lys Gln Ala Val AsnAsp Leu Val Leu Ser Lys 180 185 190 Ser Phe Asp Asn Gly Met Ile Cys AlaSer Glu Gln Gly Ala Ile Val 195 200 205 Asp Ser Ser Ile Tyr Asp Ala AlaLys Lys Glu Phe Glu Ala Gln Gly 210 215 220 Ala Tyr Phe Val Lys Pro LysAsp Met Lys Lys Phe Glu Ser Thr Val 225 230 235 240 Ile Asn Leu Glu LysGln Ser Val Asn Pro Arg Ile Val Gly Gln Ser 245 250 255 Pro Lys Gln IleAla Glu Trp Ala Gly Ile Arg Ile Pro Asp Asp Thr 260 265 270 Thr Ile LeuIle Ala Glu Leu Lys Asp Val Gly Lys Lys Tyr Pro Leu 275 280 285 Ser ArgGlu Lys Leu Ser Pro Val Leu Ala Met Val Lys Ala Asp Gly 290 295 300 HisGlu Asp Ala Phe Lys Lys Cys Glu Thr Met Leu Asp Ile Gly Gly 305 310 315320 Leu Gly His Thr Ala Val Ile His Thr Ala Asp Asp Glu Leu Ala Leu 325330 335 Lys Phe Ala Asp Thr Met Gln Ala Cys Arg Ile Leu Ile Asn Thr Pro340 345 350 Ser Ser Val Gly Gly Ile Gly Asp Leu Tyr Asn Glu Met Ile ProSer 355 360 365 Leu Thr Leu Gly Cys Gly Ser Tyr Gly Gly Asn Ser Ile SerHis Asn 370 375 380 Val Gly Thr Val Asp Leu Leu Asn Ile Lys Thr Met AlaLys Arg Arg 385 390 395 400 Asn Asn Met Gln Trp Met Lys Leu Pro Pro LysIle Tyr Phe Glu Lys 405 410 415 Asn Ser Val Arg Tyr Leu Glu His Met GluSer Ile Lys Arg Ala Phe 420 425 430 Ile Val Ala Asp Arg Ser Met Glu LysAla Gly Phe Arg Gln Asp His 435 440 445 85 158 PRT Lactobacillusrhamnosus 85 Val Leu Val Asn Asn Ala Gly Ile Thr Asp Asp Met Leu Ala MetArg 1 5 10 15 Met Lys Pro Ala Ser Phe Ala Lys Val Val Gln Val Asn LeuAsp Gly 20 25 30 Thr Phe Tyr Val Thr Gln Pro Ala Phe Lys Lys Met Leu LysAla Arg 35 40 45 Ala Gly Val Ile Ile Asn Leu Ala Ser Val Val Gly Leu ThrGly Asn 50 55 60 Ile Gly Gln Ala Asn Tyr Ala Ala Ser Lys Ala Gly Ile IleGly Leu 65 70 75 80 Thr Lys Thr Leu Ala Arg Glu Gly Ala Met Arg Gly ValArg Val Asn 85 90 95 Ala Ile Ala Pro Gly Met Ile Ala Thr Asp Met Thr AlaAla Leu Ser 100 105 110 Gln Ser Ser Gln Asp Gln Ile Leu Ala Glu Ile ProLeu Lys Arg Phe 115 120 125 Gly Gln Pro Glu Glu Ile Ala His Thr Ala ArgPhe Leu Val Glu Asn 130 135 140 Ala Tyr Ile Thr Gly Gln Thr Val Thr ValAla Gly Gly Leu 145 150 155 86 334 PRT Lactobacillus rhamnosus 86 MetTyr His Ala Ala Ala Asp Arg Tyr Glu Lys Met Pro Val Arg His 1 5 10 15Ala Gly Lys Thr Gly Leu Met Leu Pro Val Ile Ser Leu Gly Leu Trp 20 25 30Gln His Tyr Gly Asn Leu Asp Pro Phe Gly Pro Arg Arg Ser Val Ile 35 40 45Leu Asp Ala Phe Asp Arg Gly Val Phe His Phe Asp Val Ala Asn His 50 55 60Tyr Gly Asn Gly Asp Arg Glu Pro Gly Phe Gly Ser Ser Glu Arg Leu 65 70 7580 Leu Gly Gln Ile Leu Ala Thr Asp Leu Lys Pro Tyr Arg Asp Glu Leu 85 9095 Val Ile Ser Thr Lys Val Gly Tyr Glu Ile His Pro Gly Pro Tyr Gly 100105 110 Val Gly Thr Ser Arg Lys Ala Val Ile Gln Gly Leu Asn Asp Ser Leu115 120 125 Lys Arg Leu Gln Leu Asp Tyr Val Asp Ile Tyr Tyr Ala His ArgPhe 130 135 140 Asp Asp Thr Val Ala Leu Glu Glu Thr Val Asn Ala Leu AspGln Thr 145 150 155 160 Val Arg Asp Gly Lys Ala Leu Tyr Ile Gly Ile SerAsn Tyr Asp Thr 165 170 175 Lys Gln Thr Lys Glu Ala Ile Ala Met Phe LysAsp Leu His Thr Pro 180 185 190 Phe Val Leu Asn Gln Tyr Ser Tyr Asn MetPhe Asn Arg Thr Ala Glu 195 200 205 Thr Ser Gly Leu Ile Asp Ala Leu LysAla Asp Gly Ala Gly Leu Ile 210 215 220 Ala Tyr Gly Pro Leu Ser Glu GlyLeu Leu Ser Asp Arg Tyr Leu Lys 225 230 235 240 Gly Ile Pro Asp Thr PheLys Ile His Pro Thr Asn Lys Ala Thr Phe 245 250 255 Ala Lys Gly Lys GluAla Val Val Lys Gln Leu Asn Ala Leu Asn Glu 260 265 270 Ile Ala His AspArg Asp Gln Thr Leu Ser Gln Met Ala Leu Ala Trp 275 280 285 Leu Leu ArgAsp Pro Val Val Thr Ser Val Ile Ile Gly Thr Thr Ser 290 295 300 Val GluHis Leu Gln Asp Asn Leu Lys Ala Thr Glu His Leu Thr Phe 305 310 315 320Thr Ala Glu Glu Ile Gln Gln Ile Asp Asp Ile Leu Asn Ala 325 330 87 413PRT Lactobacillus rhamnosus 87 Ala Val Ala Leu Pro Leu Leu Gly Val LeuAla Ile Ala Ala Thr His 1 5 10 15 Ala Glu Gly Val Tyr Asp Ile Gly ArgPro Leu Gly Arg Phe Phe Ala 20 25 30 Leu Ala Phe Met Val Leu Ile His AlaThr Ile Gly Pro Met Phe Gly 35 40 45 Thr Pro Arg Thr Ala Thr Val Ser PheThr Thr Gly Val Leu Pro Met 50 55 60 Leu Pro Lys Ala Trp Gln Gln Gly GlyLeu Leu Val Phe Ser Ala Leu 65 70 75 80 Phe Phe Gly Ala Ala Phe Phe LeuSer Tyr Lys Glu Arg Lys Ile Thr 85 90 95 Thr Ala Val Gly Lys Val Leu AsnPro Val Phe Leu Leu Leu Leu Phe 100 105 110 Phe Val Phe Phe Ile Gly PheLeu His Pro Met Gly Asn Pro Ala Ala 115 120 125 Gln Thr Val Thr Ala AlaTyr Lys Asn Gly Gly Ser Phe Met Ser Gly 130 135 140 Phe Leu Gln Gly TyrAsn Thr Met Asp Ala Leu Ala Ala Leu Ala Phe 145 150 155 160 Gly Val ThrVal Val Thr Ala Val Arg Gly Leu Gly Leu Lys Asn Asp 165 170 175 Asp HisVal Ala Lys Ala Thr Ala Lys Ala Gly Val Met Ala Thr Ser 180 185 190 TrpIle Ala Leu Ile Tyr Val Ala Leu Ile Val Leu Gly Ser Met Ser 195 200 205Leu Ala His Phe Lys Leu Ser Ala Glu Gly Gly Thr Ala Phe Asn Gln 210 215220 Val Gly Thr Phe Tyr Phe Gly Thr Val Gly His Pro Ala Trp Gln Pro 225230 235 240 Cys Leu Thr Leu Thr Cys Leu Asn Thr Pro Val Gly Phe Val ArgAla 245 250 255 Phe Pro His Asp Phe His Arg His Phe Pro Lys Val Ser TyrGln Val 260 265 270 Trp Leu Gly Leu Thr Ser Phe Leu Ser Phe Leu Thr AlaAsn Phe Gly 275 280 285 Leu Glu Gln Ile Ile Ala Trp Ser Val Pro Met LeuMet Phe Leu Tyr 290 295 300 Pro Phe Ser Met Val Leu Ile Leu Leu Ser ValPhe Gly Lys Ala Phe 305 310 315 320 His His Asp Pro Leu Val Tyr Arg IleVal Val Ala Phe Thr Ile Val 325 330 335 Pro Ala Val Leu Asp Met Phe AlaAla Phe Pro Ala Val Val Ser Gln 340 345 350 Ser Ser Leu Gly Leu Ala LeuHis Ser Phe Gln Leu His Phe Leu Pro 355 360 365 Phe Ser Ala Met Gly LeuGly Trp Leu Val Pro Ala Gly Val Gly Leu 370 375 380 Val Leu Gly Leu ValAla His Ala Val Lys Val Arg Lys Ala Val Ala 385 390 395 400 Ala Thr HisLeu Glu Ala Glu Gln Thr Gln Leu Val His 405 410 88 627 PRT Lactobacillusrhamnosus 88 Met Ala Asp Asn His Lys Ala Gln Thr Thr Lys Gln Pro Ser GlyPro 1 5 10 15 Arg Met Gly Pro Gly Arg Gly Gly Leu Val Glu Lys Pro LysAsn Phe 20 25 30 Trp Gly Thr Thr Ala Arg Leu Phe Gly Tyr Met Arg Asn ArgLeu Ile 35 40 45 Gly Ile Ile Ala Val Leu Val Leu Ala Ile Ala Ser Thr ValPhe Gln 50 55 60 Ile Arg Thr Pro Lys Ile Leu Gly Glu Ala Thr Thr Glu IlePhe Lys 65 70 75 80 Gly Val Met Lys Gly Gln Ala Glu Gln Lys Ala Gly IleAla Val Gly 85 90 95 Asn Tyr Pro Ile Asp Phe Asp Lys Ile Lys Gln Ile IleLeu Ile Val 100 105 110 Leu Val Leu Tyr Leu Gly Ser Ala Leu Phe Ser PheLeu Gln Gln Phe 115 120 125 Ile Met Thr Arg Ile Ser Gln Asn Thr Val TyrGln Leu Arg Lys Asp 130 135 140 Leu Lys His Lys Met Lys Thr Val Pro IleLys Tyr Tyr Asp Thr His 145 150 155 160 Ser Asn Gly Asp Ile Met Ser ArgAla Ile Asn Asp Met Asp Asn Ile 165 170 175 Ala Ser Thr Leu Gln Gln SerLeu Thr Gln Met Val Thr Ser Ala Val 180 185 190 Met Phe Val Gly Thr IleTrp Met Met Leu Thr Ile Ser Trp Lys Leu 195 200 205 Thr Leu Ile Ala LeuVal Thr Ile Pro Leu Gly Leu Ile Val Val Gly 210 215 220 Ile Val Ala ProLys Ser Gln Arg Phe Phe Ala Ala Gln Gln Lys Ala 225 230 235 240 Leu GlyLeu Leu Asn Asn Gln Val Glu Glu Thr Tyr Gly Gly Gln Val 245 250 255 IleIle Lys Ser Phe Asn Arg Glu Asp Asp Glu Val Glu Ala Phe Glu 260 265 270Gly Gln Asn Gln Ala Phe Tyr Asp Ala Ala Trp Lys Ala Gln Phe Val 275 280285 Ser Gly Ile Ile Met Pro Leu Met Ile Phe Leu Asn Asn Ile Gly Tyr 290295 300 Val Phe Val Ala Ile Met Gly Gly Ile Glu Val Ser Asn Gly Thr Ile305 310 315 320 Thr Leu Gly Asn Val Gln Ala Phe Leu Gln Tyr Met Gln GlnPhe Ser 325 330 335 Gln Pro Ile Ser Gln Leu Ala Asn Leu Ala Asn Thr IleGln Ser Thr 340 345 350 Ile Ala Ser Ala Glu Arg Ile Phe Ala Val Leu AspGlu Glu Asp Met 355 360 365 Gln Asp Glu Pro Ser Gly Val Pro Ala Val AlaAsn Asp Pro Asn Lys 370 375 380 Leu Val Met Asp His Val Gln Phe Gly TyrThr Pro Asp Ala Leu Leu 385 390 395 400 Leu Lys Asp Tyr Asn Leu Gln ValLys Pro Gly Glu Met Val Ala Ile 405 410 415 Val Gly Pro Thr Gly Ala GlyLys Thr Thr Ile Ile Asn Leu Leu Glu 420 425 430 Arg Phe Tyr Asp Ile SerGly Gly Ser Ile Arg Leu Asn Gly Thr Asp 435 440 445 Thr Arg Asp Met LysArg Glu Asp Val Arg Ala His Phe Ala Met Val 450 455 460 Leu Gln Asp ThrTrp Leu Phe Thr Gly Thr Ile Trp Asp Asn Leu Lys 465 470 475 480 Tyr GlyArg Glu Asp Ala Thr Asp Asp Glu Val Leu Ala Ala Ala Lys 485 490 495 AlaAla His Val Asp Asn Phe Val Arg Gln Leu Pro Asp Gly Tyr Asn 500 505 510Thr Ile Leu Asn Glu Glu Ala Ser Asn Ile Ser Gln Gly Gln Arg Gln 515 520525 Leu Leu Thr Ile Ala Arg Ala Phe Val Ala Asp Pro Glu Ile Leu Ile 530535 540 Leu Asp Glu Ala Thr Ser Ser Val Asp Thr Arg Thr Glu Ile His Ile545 550 555 560 Gln His Ala Met Asn Arg Leu Leu Thr Asp Arg Thr Ser PheVal Val 565 570 575 Ala His Arg Leu Ser Thr Ile Arg Asp Ala Asp Lys IleIle Val Met 580 585 590 Asn His Gly Ser Ile Val Glu Thr Gly Asn His AspGlu Leu Met Ala 595 600 605 Lys Asn Gly Phe Tyr Ala Asp Leu Tyr Asn SerGln Phe Ser Gly Asn 610 615 620 Val Ala Ile 625 89 202 PRT Lactobacillusrhamnosus 89 Thr Thr Arg Leu Ser Ser Leu Ile Thr Glu Tyr Leu Asp Ser GlnLeu 1 5 10 15 Ala Glu Arg Arg Ser Met His Gly Val Leu Val Asp Ile TyrGly Leu 20 25 30 Gly Val Leu Ile Thr Gly Asp Ser Gly Val Gly Lys Ser GluThr Ala 35 40 45 Leu Glu Leu Val Gln Arg Gly His Arg Leu Ile Ala Asp AspArg Val 50 55 60 Asp Val Tyr Gln Gln Asp Glu Gln Thr Val Val Gly Ala AlaPro Pro 65 70 75 80 Ile Leu Ser His Leu Leu Glu Ile Arg Gly Leu Gly IleIle Asp Val 85 90 95 Met Asn Leu Phe Gly Ala Gly Ala Val Arg Glu Asp ThrThr Ile Ser 100 105 110 Leu Ile Val His Leu Glu Asn Trp Thr Pro Asp LysThr Phe Asp Arg 115 120 125 Leu Gly Ser Gly Glu Gln Thr Gln Met Ile PheAsp Val Pro Val Pro 130 135 140 Lys Ile Thr Ile Pro Val Lys Val Gly ArgAsn Leu Ala Ile Ile Ile 145 150 155 160 Glu Val Ala Ala Met Asn Phe ArgAla Lys Ser Met Gly Tyr Asp Ala 165 170 175 Thr Lys Thr Phe Glu Lys AsnLeu Asn His Leu Ile Glu His Asn Glu 180 185 190 Ala Asn Asp Gln Lys SerSer Glu Glu Lys 195 200 90 341 PRT Lactobacillus rhamnosus VARIANT(1)...(341) Xaa = Any Amino Acid 90 Met Ser Ile Ser Thr Arg Ala Asn LysLeu Asp Gly Val Glu Gln Ala 1 5 10 15 Xaa Val Ala Met Ala Thr Glu MetAsn Lys Gly Val Leu Lys Asn Leu 20 25 30 Gly Leu Leu Thr Pro Glu Leu GluGln Ala Lys Asn Gly Asp Leu Met 35 40 45 Ile Val Ile Asn Gly Lys Ser GlyAla Asp Asn Glu Gln Leu Leu Val 50 55 60 Glu Ile Glu Glu Leu Phe Asn ThrLys Ala Gln Ser Gly Ser His Glu 65 70 75 80 Ala Arg Tyr Ala Thr Ile GlySer Ala Lys Lys His Ile Pro Glu Ser 85 90 95 Asn Leu Ala Val Ile Ser ValAsn Gly Leu Phe Ala Ala Arg Glu Ala 100 105 110 Arg Gln Ala Leu Gln AsnAsp Leu Asn Val Met Leu Phe Ser Asp Asn 115 120 125 Val Ser Val Glu AspGlu Leu Ala Leu Lys Gln Leu Ala His Glu Lys 130 135 140 Gly Leu Leu MetMet Gly Pro Asp Cys Gly Thr Ala Ile Ile Asn Gly 145 150 155 160 Ala AlaLeu Cys Phe Gly Asn Ala Val Arg Arg Gly Asn Ile Gly Ile 165 170 175 ValGly Ala Ser Gly Thr Gly Ser Gln Glu Leu Ser Val Arg Ile His 180 185 190Glu Phe Gly Gly Gly Val Ser Gln Leu Ile Gly Thr Gly Gly Arg Asp 195 200205 Leu Ser Glu Lys Ile Gly Gly Leu Met Met Leu Asp Ala Ile Gly Met 210215 220 Leu Glu Asn Asp Pro Gln Thr Glu Ile Ile Ala Leu Ile Ser Lys Pro225 230 235 240 Pro Ala Pro Ala Val Ala Arg Lys Val Leu Glu Arg Ala ArgAla Cys 245 250 255 Arg Lys Pro Val Val Val Cys Phe Leu Asp Arg Gly GluThr Pro Val 260 265 270 Asp Glu Gln Gly Leu Gln Phe Ala Arg Gly Thr LysGlu Ala Ala Leu 275 280 285 Lys Ala Val Met Leu Ser Gly Val Lys Gln GluAsn Leu Asp Leu His 290 295 300 Thr Leu Asn Gln Pro Leu Ile Ala Asp ValArg Ala Arg Leu Gln Pro 305 310 315 320 Gln Gln Lys Tyr Ile Arg Gly LeuSer Ala Ala Ala Arg Cys Ala Thr 325 330 335 Lys Pro Cys Ser Arg 340 91409 PRT Lactobacillus rhamnosus 91 Gln Ile Leu Asn Asn Pro Phe Leu AsnLys Gly Thr Ala Phe Thr Gln 1 5 10 15 Glu Glu Arg Asn Gln Tyr Gly LeuAsn Gly Leu Leu Pro Pro Ala Val 20 25 30 Gln Thr Leu Asp Gln Gln Val LysGln Ala Tyr Ala Gln Leu Gln Thr 35 40 45 Lys Pro Thr Asp Leu Ala Lys ArgGln Phe Leu Met Thr Leu Phe Asn 50 55 60 Glu Asn His Val Leu Phe Tyr LysLeu Phe Ser Glu His Ile Asn Glu 65 70 75 80 Phe Met Pro Ile Val Tyr AspPro Thr Ile Ala Asp Thr Ile Glu Asn 85 90 95 Tyr Ser Ala Leu Phe Val AsnPro Gln Asn Ala Thr Tyr Leu Ser Ile 100 105 110 Asp Asp Pro Asp His IleGlu Ser Ala Leu Lys His Ser Ala Asp Gly 115 120 125 Arg Asp Ile Arg LeuLeu Val Val Ser Asp Ala Glu Gly Ile Leu Gly 130 135 140 Ile Gly Asp TrpGly Thr Gln Gly Val Asp Ile Ser Val Gly Lys Leu 145 150 155 160 Met ValTyr Thr Ala Ala Ala Gly Ile Asp Pro Ser Gln Val Leu Pro 165 170 175 ValVal Leu Asp Val Gly Thr Asn Asn Glu Gly Leu Leu Asn Asp Asp 180 185 190Leu Tyr Leu Gly Asn Arg His Lys Arg Val Tyr Gly Glu Lys Tyr His 195 200205 His Phe Val Asp Lys Phe Val Ala Ala Ala Glu Lys Leu Phe Pro Asn 210215 220 Leu Tyr Leu His Phe Glu Asp Phe Gly Arg Ser Asn Ala Ala Asp Ile225 230 235 240 Leu Asn Gln Tyr Lys Asp Lys Ile Thr Thr Phe Asn Asp AspIle Gln 245 250 255 Gly Thr Gly Ile Ile Val Leu Ala Gly Leu Leu Gly AlaMet Asn Ile 260 265 270 Ser Lys Gln Lys Leu Thr Asp Gln Val Tyr Leu SerPhe Gly Ala Gly 275 280 285 Thr Ala Gly Ala Gly Ile Ala Ser Arg Val TyrGlu Ala Phe Val Glu 290 295 300 Glu Gly Leu Ser Pro Glu Glu Ala Lys LysHis Phe Tyr Leu Val Asp 305 310 315 320 Lys Gln Gly Leu Leu Phe Asp AspMet Thr Asp Leu Thr Pro Glu Gln 325 330 335 Lys Pro Phe Ala Arg Ser ArgSer Glu Phe Ala Asn Ala Asp Glu Leu 340 345 350 Thr Thr Leu Glu Ala ValVal Lys Ala Val His Pro Thr Val Leu Val 355 360 365 Gly Thr Ser Thr ValPro Gly Thr Phe Thr Glu Ser Ile Val Lys Glu 370 375 380 Met Ala Ala HisThr Asp Arg Pro Ile Ile Phe Pro Leu Ser Asn Pro 385 390 395 400 Thr LysLeu Ala Glu Ala Lys Ala Asp 405 92 386 PRT Lactobacillus rhamnosusVARIANT (1)...(386) Xaa = Any Amino Acid 92 Met Ile Lys Pro Glu Lys ThrIle Asn Gly Thr Lys Trp Ile Glu Thr 1 5 10 15 Ile Gln Ile Asn Ala GluGlu Arg Ala Thr Leu Glu Asp Gln Tyr Gly 20 25 30 Val Asp Glu Asp Ile IleGlu Tyr Val Thr Asp Asn Asp Glu Ser Thr 35 40 45 Asn Tyr Val Tyr Asp IleAsn Glu Asp Asp Gln Leu Phe Ile Phe Leu 50 55 60 Ala Pro Tyr Ala Leu AspLys Asp Ala Leu Arg Tyr Ile Thr Gln Pro 65 70 75 80 Phe Gly Met Leu LeuHis Lys Gly Val Leu Phe Thr Phe Asn Gln Ser 85 90 95 His Ile Pro Glu ValAsn Thr Ala Leu Tyr Ser Ala Leu Asp Asn Pro 100 105 110 Glu Val Lys SerVal Asp Ala Phe Ile Leu Glu Thr Leu Phe Thr Val 115 120 125 Val Asp SerPhe Ile Pro Ile Ser Arg Gly Ile Thr Lys Lys Arg Asn 130 135 140 Tyr LeuAsp Lys Met Leu Asn Arg Lys Thr Lys Asn Ser Asp Leu Val 145 150 155 160Ser Leu Ser Tyr Leu Gln Gln Thr Leu Thr Phe Leu Ser Ser Ala Val 165 170175 Gln Thr Asn Leu Ser Glu Leu Asp Leu Asn Gly Ser Asp Ala Leu Gln 180185 190 Gln Ile Ile Glu Leu Leu Asn Gln His Pro Leu Asp Xaa Ala Pro Asp195 200 205 Glu Lys Gly Ala Tyr Ser Asn Ser Asn Tyr Tyr Leu Leu Gly HisIle 210 215 220 Ile Thr Gln Val Ala Asn Met Pro Leu Ser Asp Phe Leu AsnGln His 225 230 235 240 Phe Phe Glu Pro Leu Ala Met Thr Lys Thr Gln LeuGly Thr Gln His 245 250 255 Ala Asp Ala Asn Ser Tyr Asp Asp Leu Asp PheThr Asn Gly Lys Pro 260 265 270 Val Ala Leu Gly Arg Gly His Tyr Gln GlyGly Asp Gly Ala Val Val 275 280 285 Ser Ser Leu Ala Asp Leu Ala Ile TrpAla Arg Ala Val Leu Gln Arg 290 295 300 Arg Ile Leu Pro Glu Ser Ala TrpAsp Glu Ala Leu Thr Leu Thr His 305 310 315 320 Asp Phe Tyr Gly Met GlyTrp Met Lys Ser Arg Thr Gln His Trp Leu 325 330 335 Ser His Asn Gly HisIle Phe Gly Tyr Trp Ala Phe Phe Asp Val Ser 340 345 350 Phe Glu Lys GlnLeu Ala Gln Ile Thr Leu Thr Asn Met Ser Pro Gly 355 360 365 Val Glu ThrLeu Lys Lys Trp Gln Glu Glu Met Ala Asn Trp Arg Ala 370 375 380 Ser Leu385 93 357 PRT Lactobacillus rhamnosus 93 Leu Asp Asn Gln Asp Ala AspPhe Lys Pro Thr Ile Gln Ile Leu Asp 1 5 10 15 Glu Val Gly Lys Val ValAsn Pro Asp Ile Met Pro Asp Leu Ser Asp 20 25 30 Asp Gln Leu Val Asp LeuMet Ser Lys Met Val Trp Gln Arg Val Leu 35 40 45 Asp Gln Arg Ala Thr AlaLeu Asn Arg Gln Gly Arg Leu Gly Phe Tyr 50 55 60 Ala Pro Ser Ala Gly GluGlu Ala Ser Met Ile Gly Ser His Ala Ala 65 70 75 80 Met Lys Ser Ser AspTrp Leu Leu Pro Ala Tyr Arg Asp Leu Pro Gln 85 90 95 Leu Ile Gln His GlyLeu Pro Leu Asp Lys Ala Phe Leu Trp Ser Arg 100 105 110 Gly His Val AlaGly Asn Glu Tyr Pro Glu Asp Phe His Ala Leu Pro 115 120 125 Pro Gln IleIle Ile Gly Ala Gln Tyr Val Gln Thr Ala Gly Val Ala 130 135 140 Leu GlyLeu Lys Lys Asn Gly Ser Asp Glu Val Ala Phe Thr Tyr Thr 145 150 155 160Gly Asp Gly Gly Thr Ser Gln Gly Asp Phe Tyr Glu Gly Val Asn Phe 165 170175 Ala Gly His Phe Lys Ala Pro Ala Leu Phe Ile Val Gln Asp Asn Gly 180185 190 Phe Ala Ile Ser Val Pro Arg Ala Ser Gln Thr Ala Ala Lys Thr Leu195 200 205 Ala Gln Lys Ala Val Ala Ala Gly Val Pro Gly Val Gln Val AspGly 210 215 220 Met Asp Ala Leu Ala Val Tyr Glu Val Thr Lys Glu Ala ArgAla Trp 225 230 235 240 Ala Ala Ala Gly Asn Gly Pro Val Leu Ile Glu ThrLeu Thr Tyr Arg 245 250 255 Tyr Gly Pro His Thr Leu Ser Gly Asp Asp ProThr Arg Tyr Arg Ser 260 265 270 Lys Glu Thr Asp Glu Leu Trp Gln Lys ArgAsp Pro Leu Ile Arg Met 275 280 285 Arg Asn Tyr Leu Thr Asp Lys Gly LeuTrp Ser Lys Asp Lys Glu Asp 290 295 300 Ala Leu Ile Glu Lys Val Lys AspGlu Ile Lys Asp Ala Ile Asn Lys 305 310 315 320 Ala Asp Lys Ala Pro GlnGln Thr Val Ser Arg Phe Leu Lys Asp Thr 325 330 335 Tyr Glu Val Ala ProGln Asn Val Ala Glu Gln Leu Ala Glu Phe Gln 340 345 350 Gly Lys Glu SerLys 355 94 436 PRT Lactobacillus rhamnosus 94 Ser Val Leu Asn Ile AsnGly Gly Asn Leu Thr Leu Thr Asp Asp Gly 1 5 10 15 Val Ser Ala Gly ThrLeu Thr Gly Gly Gly Phe Leu Asn Ile Ser Gly 20 25 30 Gly Val Leu Asp IleThr Gly Gly Asn His Thr Phe Ala Val Ser Thr 35 40 45 Ile Ile Ala Lys AspAla Thr Val Arg Met Asn Asp Val Ser Gly Leu 50 55 60 Gly Thr Gly Asn IleSer Asn Ala Gly Thr Leu Ser Leu Thr His Ala 65 70 75 80 Ser Gly Leu LeuSer Asn Asn Leu Ser Gly Ser Gly Thr Val Ser Leu 85 90 95 Ile Asn Ser AspThr Gln Ile Ser Gly Asn Asn Ser Asn Tyr Ser Gly 100 105 110 Leu Phe ValVal Asp Thr Ser Ser Gln Leu Thr Ala Thr Gly Ala Gln 115 120 125 Asn LeuGly Ile Ala Ser Val Ser Asn Arg Gly Ile Leu Gln Leu Asn 130 135 140 AsnThr Thr Asp Trp Gln Leu Ile Asn Asn Val Thr Gly Thr Gly Asn 145 150 155160 Val Arg Lys Thr Gly Ser Gly Ser Leu Thr Val Arg Ser Asn Ala Ala 165170 175 Trp Ser Gly Gln Thr Asp Ile Asp Asp Gly Ser Leu Ile Leu Gly Gln180 185 190 Ser Asp Ala Pro Val Met Leu Ala Ser Ser Leu Val Asn Ile AlaLys 195 200 205 Asn Gly Lys Leu Thr Gly Phe Gly Gly Val Val Gly Asn ValThr Asn 210 215 220 Ser Gly Ser Leu Asp Leu Arg Ser Ala Ala Pro Gly AsnIle Leu Thr 225 230 235 240 Ile Gly Gly Asn Tyr Thr Gly Asn Asn Gly ThrLeu Leu Ile Asn Thr 245 250 255 Val Leu Asp Asp Ser Ser Ser Ala Thr AspLys Leu Val Ile Lys Gly 260 265 270 Asp Ala Ser Gly Lys Thr Arg Val AlaVal Thr Asn Val Gly Gly Ser 275 280 285 Gly Ala Asn Thr Leu Asn Ser IleGlu Val Ile His Val Asp Gly Asn 290 295 300 Ala Ala Asn Ala Glu Phe IleGln Ala Gly Arg Ile Ala Ala Gly Ala 305 310 315 320 Tyr Asp Tyr Thr LeuGly Arg Gly Pro Gly Ser Asn Tyr Gly Asn Trp 325 330 335 Tyr Leu Ser SerSer Lys Asn Thr Pro Glu Pro Arg Pro Asp Pro Glu 340 345 350 Pro Thr ProGlu Gly His Asp Asn Asn Leu Arg Pro Glu Ala Ser Ser 355 360 365 Tyr ThrAla Asn Ile Ala Ala Ala Asn Thr Met Phe Val Thr Arg Leu 370 375 380 HisGlu Arg Leu Gly Gln Thr Gln Tyr Val Asp Ala Ile Thr Gly Glu 385 390 395400 Pro Lys Ala Thr Ser Met Trp Met Arg His Glu Gly Gly His Asn Arg 405410 415 Trp Arg Asp Gly Ser Gly Gln Leu Lys Thr Gln Ser Asn Arg Tyr Val420 425 430 Ile Gln Leu Gly 435 95 215 PRT Lactobacillus rhamnosus 95Met Lys Ile Leu Ile Thr Gly Ala Gln Gly Gln Leu Gly Thr Glu Leu 1 5 1015 Arg His Leu Leu Asp Ala Arg Gly Ile Thr Tyr Arg Ala Thr Asp Ala 20 2530 Lys Asp Leu Asp Ile Thr Asp Glu Ala Ala Val Asn Gln Tyr Phe Ala 35 4045 Asp Tyr Gln Pro Asp Val Val Tyr His Cys Ala Ala Tyr Thr Ala Val 50 5560 Asp Lys Ala Glu Asp Glu Ala Lys Ala Leu Asn Gln Leu Val Asn Val 65 7075 80 Asp Gly Thr Arg Asn Leu Ala Lys Ala Ala Ala Lys Val Asp Ala Thr 8590 95 Leu Val Tyr Ile Ser Thr Asp Tyr Val Phe Asp Gly Asp Ser Lys Glu100 105 110 Ile Tyr Thr Val Asp Asp Gln Pro Ala Pro Arg Asn Glu Tyr GlyArg 115 120 125 Ala Lys Tyr Glu Gly Glu Gln Gln Val Gln Lys Tyr Leu LysLys Tyr 130 135 140 Tyr Ile Ile Arg Thr Ser Trp Val Phe Gly Glu Tyr GlyHis Asn Phe 145 150 155 160 Val Tyr Thr Met Leu Asn Leu Ala Lys Thr HisLys Glu Leu Thr Val 165 170 175 Val Asp Asp His Gln Glu Ser Phe Ser ValSer Ser Ser Arg Thr Phe 180 185 190 Val Lys Tyr Gln His Glu His Leu IleTyr Ser Arg Pro Val Pro Tyr 195 200 205 Arg Pro His Leu Pro Gly Ile 210215 96 640 PRT Lactobacillus rhamnosus VARIANT (1)...(640) Xaa = AnyAmino Acid 96 Met Leu Gly Gly Lys Gln Met Pro Glu Val Lys Lys Phe GluAla Gly 1 5 10 15 Thr Tyr Asp Val Ile Val Val Gly Ala Gly His Ala GlyXaa Val Lys 20 25 30 Pro Ala Leu Ala Ala Ala Arg Met Gly Glu Lys Thr LeuLeu Leu Thr 35 40 45 Ile Ser Leu Glu Met Leu Ala Phe Met Pro Cys Asn ProSer Leu Gly 50 55 60 Gly Pro Ala Lys Gly Ile Val Val Arg Glu Ile Asp AlaLeu Gly Gly 65 70 75 80 Glu Met Gly Lys Asn Ile Asp Arg Thr Tyr Ile GlnMet Arg Met Leu 85 90 95 Asn Thr Gly Lys Gly Pro Ala Val Arg Ala Leu ArgAla Gln Ala Asp 100 105 110 Lys Ala Ala Tyr His Arg Ser Met Lys His ValIle Glu Asp Thr Pro 115 120 125 His Leu Asp Leu Arg Gln Gly Leu Ala ThrGlu Val Leu Val Glu Asp 130 135 140 Gly Lys Ala Val Gly Ile Val Ala AlaThr Gly Ala Ile Tyr Arg Ala 145 150 155 160 Lys Ser Ile Val Leu Thr AlaGly Thr Ser Ser Arg Gly Lys Ile Ile 165 170 175 Ile Gly Glu Leu Met TyrSer Ser Gly Pro Asn Asn Ser Leu Pro Ser 180 185 190 Ile Lys Leu Ser GluAsn Leu Glu Gln Leu Gly Phe Lys Leu Arg Arg 195 200 205 Phe Lys Thr GlyThr Pro Pro Arg Val Asn Gly Asn Thr Ile Asp Phe 210 215 220 Ser Lys ThrGlu Glu Gln Pro Gly Asp Lys Thr Pro Asn His Phe Ser 225 230 235 240 PheThr Thr Pro Asp Ser Val Tyr Leu Lys Asp Gln Leu Ser Cys Trp 245 250 255Met Thr Tyr Thr Asn Ala Thr Thr His Gln Ile Ile Arg Glu Asn Leu 260 265270 Asp Arg Ala Pro Met Phe Ser Gly Val Ile Lys Gly Val Gly Pro Arg 275280 285 Tyr Cys Pro Ser Ile Glu Asp Lys Ile Val Arg Phe Ala Asp Lys Pro290 295 300 Arg His Gln Leu Phe Leu Glu Pro Glu Gly Arg Asp Thr Ser GluTyr 305 310 315 320 Tyr Val Gly Asp Phe Ser Thr Ser Met Pro Glu Glu IleGln Leu Lys 325 330 335 Met Leu His Ser Val Ala Gly Leu Glu His Ala GluLeu Met Arg Ala 340 345 350 Gly Tyr Ala Ile Glu Tyr Asp Val Ile Glu ProTrp Gln Leu Lys Ala 355 360 365 Thr Leu Glu Thr Lys Val Val Glu Asn LeuTyr Thr Ala Gly Gln Met 370 375 380 Asn Gly Thr Ser Gly Tyr Glu Glu AlaAla Gly Gln Gly Ile Val Ala 385 390 395 400 Gly Ile Asn Ala Ala Arg ArgAla Gln Gly Lys Gly Pro Phe Thr Leu 405 410 415 Lys Arg Ser Asp Ala TyrIle Gly Val Met Ile Asp Asp Leu Val Thr 420 425 430 Lys Gly Thr Asn GluPro Tyr Arg Leu Leu Thr Ser Arg Ala Glu Tyr 435 440 445 Arg Leu Leu LeuArg His Asp Asn Ala Asp Leu Arg Leu Thr Pro Met 450 455 460 Gly His GluLeu Gly Leu Ile Ser Asp Gln Arg Tyr Ala Val Phe Leu 465 470 475 480 AlaLys Arg Gln Ala Ile Thr Asp Glu Leu Ala Arg Leu Glu His Thr 485 490 495Arg Leu Lys Pro Lys Asp Val Asn Pro Trp Leu Glu Ala His His Phe 500 505510 Ala Ser Leu Lys Asp Gly Val Leu Ala Ser Asp Phe Leu Lys Arg Pro 515520 525 Glu Ile Asn Tyr Gln Thr Leu Glu Gln Phe Leu Pro Glu Asn Pro Thr530 535 540 Leu Asp His Arg Val Ile Glu Gln Val Glu Ile Gln Ile Lys TyrAla 545 550 555 560 Gly Tyr Ile Ala Lys Glu Glu Xaa Gln Cys Ala Lys LeuLys Arg Leu 565 570 575 Glu Gly Lys Lys Ile Pro Ala Arg Ile Asn Tyr GluAla Ile Asn Gly 580 585 590 Leu Ala Thr Glu Ala Arg Gln Lys Leu Val LysIle Gln Pro Glu Thr 595 600 605 Ile Ala Gln Ala Ser Arg Ile Ser Gly ValAsn Pro Ala Asp Val Ala 610 615 620 Ile Leu Ser Val Tyr Ile Glu Gln GlyArg Ile Ser Lys Val Ala Gln 625 630 635 640 97 254 PRT Lactobacillusrhamnosus 97 Pro Leu Ser Thr Met Met Leu Ala Gly Ile Arg Asp Ile Leu ValIle 1 5 10 15 Ser Thr Pro Arg Asp Ile Asp Arg Phe Gln Asp Leu Leu LysAsp Gly 20 25 30 Lys Gln Leu Gly Leu Asn Ile Ser Tyr Lys Ile Gln Glu LysPro Asn 35 40 45 Gly Leu Ala Glu Ala Phe Ile Val Gly Ala Asp Phe Ile GlyAsp Asp 50 55 60 Ser Val Cys Leu Ile Leu Gly Asp Asn Ile Phe Tyr Gly SerGly Leu 65 70 75 80 Ser Lys Leu Val Gln Arg Ser Ala Ala Lys Thr Thr GlyAla Thr Val 85 90 95 Phe Gly Tyr Gln Val Asn Asp Pro Glu Arg Phe Gly ValVal Ala Phe 100 105 110 Asp Glu Gln His His Val Gln Ser Ile Val Glu LysPro Glu His Pro 115 120 125 Glu Ser Asn Phe Ala Val Thr Gly Met Tyr PheTyr Asp Asn Gln Val 130 135 140 Val Asp Ile Ala Lys Asn Leu Lys Pro SerPro Arg Gly Glu Leu Glu 145 150 155 160 Ile Thr Asp Val Asn Lys Ala TyrLeu Glu Arg Gly Gln Leu Asp Val 165 170 175 Glu Leu Leu Gly Arg Gly PheAla Trp Leu Asp Thr Gly Thr His Glu 180 185 190 Ser Leu His Glu Ala AlaSer Phe Ile Glu Thr Val Gln Lys Arg Gln 195 200 205 Asn Leu Lys Ile AlaCys Leu Glu Glu Val Ala Tyr Arg Met Gly Tyr 210 215 220 Ile Asp Arg AspGln Leu Arg Lys Leu Ala Gln Pro Leu Lys Lys Asn 225 230 235 240 Asp TyrGly Gln Tyr Ile Leu Arg Leu Ala Asp Glu Glu Asp 245 250 98 312 PRTLactobacillus rhamnosus 98 Met Ala Ile Asn Leu Val Gly Ile Asn Asp AlaAsn Leu Thr Leu Ile 1 5 10 15 Glu Glu Gly Leu Asn Val Arg Ile Ser ProPhe Gly Asp Glu Leu Arg 20 25 30 Ile Ser Gly Glu Thr Glu Ala Val Ser LeuThr Leu Gln Leu Leu Glu 35 40 45 Ala Ala Thr Lys Leu Leu Ala Gln Gly IleLys Leu Ser Pro Gln Asp 50 55 60 Ile Ala Ser Ala Val Ala Met Ala Lys ArgGly Thr Leu Glu Tyr Phe 65 70 75 80 Ala Asp Met Tyr Ser Glu Thr Leu LeuArg Asp Ala Lys Gly Gln Pro 85 90 95 Ile Arg Ile Lys Asn Phe Gly Gln ArgGln Tyr Val Asp Ala Ile Lys 100 105 110 His Asn Asp Ile Thr Phe Gly IleGly Pro Ala Gly Thr Gly Lys Thr 115 120 125 Phe Leu Ala Val Val Met AlaVal Ala Ala Met Lys Ala Gly Gln Val 130 135 140 Glu Arg Ile Ile Leu ThrArg Pro Ala Val Glu Ala Gly Glu Ser Leu 145 150 155 160 Gly Phe Leu ProGly Asp Leu Lys Glu Lys Val Asp Pro Tyr Leu Arg 165 170 175 Pro Val TyrAsp Ala Leu Tyr Ala Val Leu Gly Lys Glu His Thr Asp 180 185 190 Arg LeuMet Asp Arg Gly Val Ile Glu Ile Ala Pro Leu Ala Tyr Met 195 200 205 ArgGly Arg Thr Leu Asp Asn Ala Phe Ala Ile Leu Asp Glu Ala Gln 210 215 220Asn Thr Thr Gln Ala Gln Met Lys Met Phe Leu Thr Arg Leu Gly Phe 225 230235 240 Gly Ser Lys Met Ile Val Asn Gly Asp Val Thr Gln Ile Asp Leu Pro245 250 255 His Asn Ala Lys Ser Gly Leu Leu Gln Ala Glu Gln Leu Leu LysGly 260 265 270 Ile Ser His Ile Ala Phe Thr Gln Phe Ser Ala Gln Asp ValVal Arg 275 280 285 His Pro Val Val Ala Lys Ile Ile Glu Ala Tyr Gly LysHis Asp Leu 290 295 300 Gln Leu Gln Lys Gln Thr Lys Glu 305 310 99 280PRT Lactobacillus rhamnosus 99 Met Lys Lys Phe Asp Lys Met Met Asp TrpLeu Ala Asp Val Tyr Val 1 5 10 15 Asn Ala Leu Asn Val Ile His Tyr MetHis Asp Lys Tyr Tyr Tyr Glu 20 25 30 Ala Ala Gln Leu Ala Leu Lys Asp ThrArg Leu Asn Arg Thr Phe Ala 35 40 45 Thr Gly Ile Ser Gly Leu Ser His AlaVal Asp Ser Ile Ser Ala Ile 50 55 60 Lys Tyr Gly His Val Lys Ala Ile ArgAsp Glu Asn Gly Val Ala Ile 65 70 75 80 Asp Phe Val Ala Asp Asn Asp AspTyr Pro Arg Tyr Gly Asn Asn Asp 85 90 95 Asp Arg Ala Asp Asn Ile Ala LysTrp Leu Val Lys Thr Phe Tyr Asn 100 105 110 Lys Met Asn Thr His His LeuTyr Arg Gly Ala Lys Leu Ser Thr Ser 115 120 125 Val Leu Thr Ile Thr SerAsn Val Val Tyr Gly Lys Asn Thr Gly Thr 130 135 140 Thr Pro Asn Gly ArgGln Lys Gly Glu Pro Phe Ser Pro Gly Ala Asn 145 150 155 160 Pro Ala TyrGly Ala Glu Lys Asn Gly Ala Leu Ala Ser Leu Met Ser 165 170 175 Thr AlaLys Ile Pro Tyr His Tyr Ala Thr Asp Gly Ile Ser Asn Thr 180 185 190 PheGly Val Thr Pro Asn Thr Leu Gly His Asp Asp Glu Thr Arg Lys 195 200 205Asp Thr Leu Val His Met Val Asp Gly Tyr Met Glu Asn Ser Gly Met 210 215220 His Leu Asn Ile Asn Val Phe Asn Lys Glu Thr Leu Ile Asp Ala Gln 225230 235 240 Lys His Pro Glu Glu Tyr Pro Thr Leu Thr Val Arg Val Ser GlyTyr 245 250 255 Cys Val Tyr Phe Ala Asp Leu Thr Lys Glu Gln Gln Asp AspVal Ile 260 265 270 Ala Arg Thr Phe Phe Asp Glu Met 275 280 100 447 PRTLactobacillus rhamnosus 100 Met Ala Phe Ser Lys Glu Thr Arg Thr Gln ThrIle Asp Gln Leu Lys 1 5 10 15 Gln Thr Glu Leu Asp Leu Leu Ile Val GlyGly Gly Ile Thr Gly Ala 20 25 30 Gly Val Ala Ile Gln Ala Ala Ala Ser GlyLeu Lys Thr Gly Leu Ile 35 40 45 Glu Met Gln Asp Phe Ala Glu Gly Thr SerSer Arg Ser Thr Lys Leu 50 55 60 Val His Gly Gly Ile Arg Tyr Leu Lys ThrPhe Asp Val Gly Val Val 65 70 75 80 Ala Asp Thr Val Lys Glu Arg Ala ValVal Gln Gly Ile Ala Pro His 85 90 95 Ile Pro Arg Pro Phe Pro Met Leu LeuPro Ile Tyr Gln Glu Ala Gly 100 105 110 Ser Thr Phe Asp Met Phe Ser IleLys Ile Ala Met Asp Leu Tyr Asp 115 120 125 Arg Leu Ala Asn Val Glu GlySer Gln Tyr Ala Asn Tyr Thr Val Thr 130 135 140 Lys Asp Glu Ile Leu GlnArg Glu Pro His Leu Ala Ser Asp Gly Leu 145 150 155 160 Gln Gly Gly GlyVal Tyr Leu Asp Phe Val Asn Asn Asp Ala Arg Leu 165 170 175 Val Ile GluAsn Ile Lys Glu Ala Ala Glu Leu Gly Gly Leu Met Ala 180 185 190 Ser ArgVal Gln Ala Ile Gly Val Leu His Asp Asp Ala Gly Gln Val 195 200 205 AsnGly Leu Gln Val Lys Asp Leu Leu Asp Gly Ser Val Phe Asp Ile 210 215 220His Ala Lys Leu Val Ile Asn Thr Thr Gly Pro Trp Ser Asp Lys Phe 225 230235 240 Lys Ala Leu Asp Gln Ala Glu Asp Gln Thr Pro Thr Leu Arg Pro Thr245 250 255 Lys Gly Val His Leu Val Val Asp Gly Ser Arg Leu Pro Val ProGln 260 265 270 Pro Thr Tyr Met Asp Thr Gly Leu Asn Asp Gly Arg Met PhePhe Val 275 280 285 Val Pro Arg Glu Gly Lys Thr Tyr Phe Gly Thr Thr AspThr Asp Tyr 290 295 300 His Gly Asp Phe Asn His Pro Gln Val Glu Gln AlaAsp Val Asp Tyr 305 310 315 320 Leu Leu Lys Val Ile Asn Lys Arg Tyr ProGln Ser His Ile Thr Leu 325 330 335 Asp Asp Ile Glu Ala Ser Trp Ala GlyLeu Arg Pro Leu Ile Ala Asn 340 345 350 Asn Gly Ser Ser Asp Tyr Asn GlyGly Gly Ala Asn Thr Gly Lys Val 355 360 365 Ser Asp Asp Ser Phe Glu AlaLeu Ile Arg Val Val Asp Asp Tyr Glu 370 375 380 Asp Asn Gln Ala Thr ArgAla Asp Val Glu His Ala Ile Ser Lys Leu 385 390 395 400 Glu Thr Ala HisAla Glu Ala Ala Leu Ser Pro Ser Gln Val Ser Arg 405 410 415 Gly Ser SerLeu Arg Gln Ala Asp Asp Gly Met Ile Thr Leu Ser Gly 420 425 430 Gly LysIle Thr Asp Tyr Arg Lys Met Ala Ala Gly Ala Leu Ala 435 440 445 101 242PRT Lactobacillus rhamnosus 101 Asp Leu Phe Cys Pro Asp Ile Thr Ala AspIle Leu Thr Arg Lys Asp 1 5 10 15 Asp Leu Gly Ser Asp Lys Pro Ile ValAsp Val Ile Leu Asp Arg Ala 20 25 30 Gly Asn Lys Gly Thr Gly Lys Trp SerSer Gln Ser Ala Leu Glu Leu 35 40 45 Gly Val Pro Gln Ser Val Ile Thr GluSer Val Tyr Ala Arg Tyr Ile 50 55 60 Ser Ala Met Lys Gln Glu Arg Val AlaAla Ser Lys Val Leu Pro Lys 65 70 75 80 Pro Val Gly Asn Val Thr Ile AspLys Lys Glu Ala Ile Glu Met Ile 85 90 95 Arg Lys Ala Leu Tyr Phe Ser LysLeu Met Ser Tyr Ala Gln Gly Phe 100 105 110 Glu Gln Met Arg Val Ala SerAsp Asn Tyr Asp Trp Asn Leu Gln Tyr 115 120 125 Gly Glu Leu Ala Lys IleTrp Arg Ala Gly Cys Ile Ile Arg Ala Arg 130 135 140 Phe Leu Gln Asn IleThr Asp Ala Tyr Asp Lys Lys Pro Asp Leu Gln 145 150 155 160 Asn Leu LeuLeu Asp Asp Tyr Phe Leu Asn Ile Ala Lys Asn Tyr Gln 165 170 175 Glu SerVal Arg Asp Leu Val Gly Leu Ala Val Lys Ala Gly Val Pro 180 185 190 ValPro Gly Phe Ser Ala Ala Ile Ser Tyr Tyr Asp Ser Tyr Arg Ala 195 200 205Pro Val Leu Pro Ala Asn Leu Thr Gln Ala Gln Arg Asp Tyr Phe Gly 210 215220 Ala His Thr Tyr Glu Arg Thr Asp Arg Asp Gly Ile Phe His Tyr Thr 225230 235 240 Trp Tyr 102 323 PRT Lactobacillus rhamnosus 102 Glu Asp PhePhe Ile Gln Ile Ser Ala Thr Gln His His Ile Pro Asp 1 5 10 15 Cys CysAsp Gln Ile Pro Thr Gly Asp Phe Ser Phe Phe Asp Asn Thr 20 25 30 Leu AspVal Ala Asn Leu Leu Asn Ile Val Pro Lys Arg Tyr Gln Asp 35 40 45 Leu AsnLeu Ser Pro Leu Asp Thr Tyr Phe Ala Gln Ala Arg Gly Tyr 50 55 60 Gln GlyGlu Ala Gly Asp Val Lys Ala Leu Ala Met Lys Lys Trp Phe 65 70 75 80 AsnThr Asn Tyr His Tyr Leu Val Pro Glu Phe Asp Arg Asp Thr Lys 85 90 95 IleGln Val Thr Asp Trp Gln Leu Phe Val Gln Phe Glu Glu Ala Lys 100 105 110Ala Leu Gly Ile Asn Gly Arg Pro Thr Leu Ile Gly Pro Tyr Thr Leu 115 120125 Leu Lys Leu Ser Arg Phe Ile Asp Val Val Pro Asp Asp Phe Val Ala 130135 140 Asp Leu Ile Ser Ala Tyr Thr Thr Ile Ile Asp Arg Leu His Asp Ala145 150 155 160 Gly Ala Asp Trp Val Gln Leu Asp Glu Pro Ala Leu Val TyrAsp Gln 165 170 175 Thr Asp Ala Asp Leu Ala Leu Phe Glu Arg Leu Tyr ThrPro Ile Leu 180 185 190 Thr Gln Lys Lys Ala Ala Lys Ile Leu Val Gln ThrTyr Phe Gly Asp 195 200 205 Leu Thr Asp Ser Phe Asp Arg Ile Gln Lys LeuPro Phe Asp Gly Phe 210 215 220 Gly Leu Asp Phe Val Glu Gly Tyr Ala AsnLeu Asp Leu Leu Lys Gln 225 230 235 240 His Gly Phe Pro Ala His Ala ThrLeu Phe Ala Gly Ile Val Asn Gly 245 250 255 Lys Asn Ile Trp Arg Thr HisTyr Ala Asp Ala Leu Ala Thr Ile Lys 260 265 270 Gln Leu Ala Thr Ile ThrAsp Lys Leu Val Leu Ser Thr Ser Thr Ser 275 280 285 Leu Leu His Val ProTyr Thr Leu Arg Asn Glu Thr His Leu Lys Pro 290 295 300 Glu Glu Lys GlnTyr Leu Ala Phe Ala Glu Glu Lys Leu Asn Glu Leu 305 310 315 320 His GluLeu 103 296 PRT Lactobacillus rhamnosus 103 Gly Pro Ala Ile Phe Gly PheIle Pro Met Gln Asp Gly Ser Pro Ala 1 5 10 15 Pro Gly Leu Ser Asn IleThr Ala Glu Gly Trp Phe Pro His Gly Gly 20 25 30 Leu Pro Ile Leu Met ThrMet Val Ala Val Asn Phe Ala Phe Ser Gly 35 40 45 Thr Glu Leu Ile Gly IleAla Ala Gly Glu Thr Glu Asn Pro Arg Lys 50 55 60 Val Ile Pro Val Ala IleArg Thr Thr Ile Ala Arg Leu Ile Ile Phe 65 70 75 80 Phe Ile Gly Thr ValPhe Val Leu Ala Ala Leu Ile Pro Met Gln Gln 85 90 95 Val Gly Val Glu LysSer Pro Phe Val Leu Val Phe Glu Lys Val Gly 100 105 110 Ile Pro Tyr AlaAla Asp Ile Phe Asn Phe Val Ile Leu Thr Ala Ile 115 120 125 Leu Cys AlaAla Asn Ser Gly Leu Tyr Ala Ser Gly Arg Met Leu Trp 130 135 140 Ser LeuSer Asn Glu Arg Thr Leu Pro Ala Cys Phe Ala Arg Val Thr 145 150 155 160Lys Asn Gly Val Pro Leu Thr Ala Leu Ser Val Ser Met Leu Gly Gly 165 170175 Val Leu Ala Leu Phe Ser Ser Val Val Ala Pro Asn Thr Val Phe Val 180185 190 Ala Leu Ser Ala Ile Ser Gly Phe Ala Val Val Ala Val Trp Leu Ser195 200 205 Ile Cys Ala Ser His Phe Val Phe Arg Arg Arg His Leu Gln GlnGly 210 215 220 Lys Ala Leu Ser Glu Leu His Tyr Arg Ala Pro Trp Tyr ProLeu Val 225 230 235 240 Pro Val Leu Gly Phe Val Leu Cys Leu Val Ala CysVal Gly Leu Ala 245 250 255 Phe Asp Pro Ala Gln Arg Ile Ala Leu Trp CysGly Leu Pro Phe Val 260 265 270 Ala Leu Cys Tyr Gly Ala Tyr Phe Leu ThrGln Pro Arg Asn Ala Lys 275 280 285 Gln Glu Pro Glu His Val Ala Glu 290295 104 474 PRT Lactobacillus rhamnosus 104 Met Arg Lys Gln Leu Pro LysAsp Phe Val Ile Gly Gly Ala Thr Ala 1 5 10 15 Ala Tyr Gln Val Glu GlyAla Thr Lys Glu Asp Gly Lys Gly Arg Val 20 25 30 Leu Trp Asp Asp Phe LeuGlu Lys Gln Gly Arg Phe Ser Pro Asp Pro 35 40 45 Ala Ala Asp Phe Tyr HisArg Tyr Asp Glu Asp Leu Ala Leu Ala Glu 50 55 60 Ala Tyr Gly His Gln ValIle Arg Leu Ser Ile Ala Trp Ser Arg Ile 65 70 75 80 Phe Ser Asp Gly AlaGly Ala Val Glu Ser Arg Gly Val Ala Phe Tyr 85 90 95 His Arg Leu Phe AlaAla Cys Ala Lys His His Leu Ile Pro Phe Val 100 105 110 Thr Leu His HisPhe Asp Thr Pro Glu Arg Leu His Glu Ile Gly Asp 115 120 125 Trp Leu SerGln Glu Met Leu Glu Asp Phe Val Glu Tyr Ala Arg Phe 130 135 140 Cys PheGlu Glu Phe Pro Glu Ile Lys His Trp Ile Thr Ile Asn Glu 145 150 155 160Pro Thr Ser Met Ala Val Gln Gln Tyr Thr Ser Gly Thr Phe Pro Pro 165 170175 Ala Glu Thr Gly His Phe Asp Lys Thr Phe Gln Ala Glu His Asn Gln 180185 190 Ile Val Ala His Ala Arg Ile Val Asn Leu Tyr Lys Ser Met Gly Leu195 200 205 Asp Gly Glu Ile Gly Ile Val His Ala Leu Gln Thr Pro Tyr ProTyr 210 215 220 Ser Asp Ser Ser Glu Asp Gln His Ala Ala Asp Leu Gln AspAla Leu 225 230 235 240 Glu Asn Arg Leu Tyr Leu Asp Gly Thr Leu Ala GlyAsp Tyr Ala Pro 245 250 255 Lys Thr Leu Ala Leu Ile Lys Glu Ile Leu AlaAla Asn Gln Gln Pro 260 265 270 Met Phe Lys Tyr Thr Asp Glu Glu Met AlaAla Ile Lys Lys Ala Ala 275 280 285 His Gln Leu Asp Phe Val Gly Val AsnAsn Tyr Phe Ser Lys Trp Leu 290 295 300 Arg Ala Tyr His Gly Lys Ser GluThr Ile His Asn Gly Asp Gly Ser 305 310 315 320 Lys Gly Ser Ser Val AlaArg Leu His Gly Ile Gly Glu Glu Lys Lys 325 330 335 Pro Ala Gly Ile GluThr Thr Asp Trp Asp Trp Ser Ile Tyr Pro Arg 340 345 350 Gly Met Tyr AspMet Leu Met Arg Ile His Gln Asp Tyr Pro Leu Val 355 360 365 Pro Ala IleTyr Val Thr Glu Asn Gly Ile Gly Leu Lys Glu Ser Leu 370 375 380 Pro AlaGlu Val Thr Pro Asn Thr Val Ile Ala Asp Pro Lys Arg Ile 385 390 395 400Asp Tyr Leu Lys Lys Tyr Leu Ser Ala Ile Ala Asp Ala Ile Gln Ala 405 410415 Gly Ala Asn Val Lys Gly Tyr Phe Val Trp Ser Leu Gln Asp Gln Phe 420425 430 Ser Trp Thr Asn Gly Tyr Ser Lys Arg Tyr Gly Leu Phe Phe Val Asp435 440 445 Phe Pro Thr Gln Lys Arg Tyr Val Lys Gln Ser Ala Glu Trp LeuLys 450 455 460 Gln Val Ser Gln Thr His Val Ile Pro Glu 465 470 105 258PRT Lactobacillus rhamnosus 105 Met Thr Thr Leu Lys Ser Phe Arg Val IleAsn Lys Val Asp Leu Pro 1 5 10 15 Ser Ala Gln Pro Asp Val Val Lys GluGlu Ile Glu Glu Met Ile Gly 20 25 30 Leu Asp Ala Ser Asp Ala Ile Leu AlaSer Gly Lys Thr Gly Leu Gly 35 40 45 Val Pro Glu Ile Leu Glu Arg Ile ValSer Asp Ile Pro Ala Pro Ser 50 55 60 Gly Asp Val Asn Ala Pro Leu Gln AlaLeu Ile Phe Asp Ser Val Tyr 65 70 75 80 Asp Asp Tyr Arg Gly Val Val LeuAsp Val Arg Val Lys Glu Gly Gln 85 90 95 Val Lys Val Gly Asp Thr Ile GlnLeu Met Ser Asn Gly Lys Gln Phe 100 105 110 Gln Val Thr Glu Val Gly ValMet Ser Pro Lys Ala Val Lys Arg Asp 115 120 125 Phe Leu Met Val Gly AspVal Gly Tyr Ile Thr Ala Ser Ile Lys Thr 130 135 140 Ile Gln Asp Thr ArgVal Gly Asp Thr Val Thr Leu Ala Asp Arg Pro 145 150 155 160 Ala Ala AlaPro Leu Lys Gly Tyr Arg Lys Ile Thr Pro Met Val Tyr 165 170 175 Ser GlyLeu Phe Pro Val Asp Asn Ala Lys Phe Asn Asp Leu Arg Glu 180 185 190 AlaLeu Glu Lys Leu Gln Leu Asn Asp Ala Ala Leu Glu Phe Glu Pro 195 200 205Glu Thr Ser Gln Ala Leu Gly Phe Gly Phe Arg Cys Gly Phe Leu Gly 210 215220 Leu Leu His Met Asp Val Val Gln Glu Arg Leu Glu Arg Asp Tyr Gly 225230 235 240 Leu Asp Leu Ile Met Thr Ala Pro Ser Val Asp Tyr Gln Val AlaLeu 245 250 255 Thr Asp 106 418 PRT Lactobacillus rhamnosus 106 Met AspVal Thr Thr Ile Asp Leu Glu Gln Met Gly Arg Ala Ala Lys 1 5 10 15 AlaAla Ala Thr Val Leu Ser Gln Leu Thr Thr Ala Gln Lys Asn Ala 20 25 30 GlyLeu Leu Ala Met Val Thr Ala Leu Glu Thr His Thr Glu Thr Ile 35 40 45 LeuGly Ala Asn His Glu Asp Leu Lys Ala Ala Ala Ser Leu Pro Ala 50 55 60 LysPhe Thr Asp Arg Leu Val Leu Thr Ala Glu Arg Ile Ala Asp Met 65 70 75 80Ala Ala Gly Val Arg Gln Val Ala Ala Leu Pro Asp Pro Thr Ala Gln 85 90 95Thr Asp Lys Ala Trp Val Asn His Ala Gly Leu Asn Ile Ala Gln Lys 100 105110 Arg Val Pro Leu Gly Val Val Gly Met Ile Tyr Glu Ala Arg Pro Asn 115120 125 Val Thr Val Asp Ala Ala Ala Leu Thr Phe Lys Ser Gly Asn Ala Val130 135 140 Ile Leu Arg Gly Gly Lys Glu Ala Leu His Ser Asn Leu Ala LeuAla 145 150 155 160 Thr Val Leu Gln Ala Ala Leu Thr Ala Gln Gly Leu ProLys Asp Ala 165 170 175 Ile Gln Leu Ile Thr Asp Pro Lys Arg Glu Val AlaAsn Gln Met Met 180 185 190 His Leu Asn Gly Tyr Ile Asp Val Leu Ile ProArg Gly Gly Arg Gly 195 200 205 Leu Ile Lys Ala Val Val Glu Gln Ala ThrVal Pro Val Ile Glu Thr 210 215 220 Gly Ala Gly Asn Cys His Ile Tyr ValAsp Ala Tyr Ala Gln Ala Gln 225 230 235 240 Met Ala Ile Asp Ile Val ValAsn Ala Lys Val Gln Arg Pro Ser Val 245 250 255 Cys Asn Ala Ala Glu LysLeu Leu Ile His Ala Asp Val Ala Asn Ala 260 265 270 Gln Leu Pro Leu IleAla Ala Ala Leu Gln Ala His Gly Val Glu Leu 275 280 285 Arg Gly Asp GluArg Ala Arg Ala Ile Val Pro Asn Met Gln Ile Ala 290 295 300 Thr Glu GluAsp Trp Asp Thr Glu Tyr Asn Asp Leu Ile Met Ala Val 305 310 315 320 LysVal Val Asp Ser Glu Glu Glu Ala Ile Ala His Ile Asn Ala His 325 330 335Asn Thr Lys His Ser Glu Ala Ile Ile Thr Asp Asn Tyr Gln Asn Ser 340 345350 Gln Gln Phe Leu Gln Gln Val Asp Ala Ala Val Val Tyr Val Asn Ala 355360 365 Ser Thr Arg Phe Thr Asp Gly Phe Glu Phe Gly Phe Gly Ala Glu Ile370 375 380 Gly Ile Ser Thr Gln Lys Leu His Ala Arg Gly Pro Met Gly LeuAla 385 390 395 400 Ala Leu Thr Thr Ile Lys Tyr Gln Val Leu Gly Asn GlyGln Val Arg 405 410 415 Glu Gly 107 163 PRT Lactobacillus rhamnosus 107Met Thr Ala Phe Leu Trp Ala Gln Asp Arg Asp Gly Leu Ile Gly Lys 1 5 1015 Asp Gly His Leu Pro Trp His Leu Pro Asp Asp Leu His Tyr Phe Arg 20 2530 Ala Gln Thr Val Gly Lys Ile Met Val Val Gly Arg Arg Thr Tyr Glu 35 4045 Ser Phe Pro Lys Arg Pro Leu Pro Glu Arg Thr Asn Val Val Leu Thr 50 5560 His Gln Glu Asp Tyr Gln Ala Pro Gly Ala Val Val Val His Asp Val 65 7075 80 Ala Ala Val Phe Ala Tyr Ala Lys Gln His Pro Asp Gln Glu Leu Val 8590 95 Ile Ala Gly Gly Ala Gln Val Phe Thr Ala Phe Lys Asp Asp Val Asp100 105 110 Thr Leu Leu Val Thr Arg Leu Ala Gly Ser Phe Glu Gly Asp ThrLys 115 120 125 Met Ile Pro Leu Asn Trp Asp Asp Phe Thr Lys Val Ser SerArg Thr 130 135 140 Val Glu Asp Thr Asn Pro Ala Leu Thr His Thr Tyr GluVal Trp Gln 145 150 155 160 Lys Lys Ala 108 95 PRT Lactobacillusrhamnosus 108 Gln Gly Cys Asn Leu Tyr Gly Ile Ala Thr Ala Leu Ala ArgIle Ser 1 5 10 15 Lys Ala Ile Leu Asn Asp Glu Asn Ala Val Leu Pro LeuSer Val Tyr 20 25 30 Met Asp Gly Gln Tyr Gly Leu Asn Asp Ile Tyr Ile GlyThr Pro Ala 35 40 45 Val Ile Asn Arg Asn Gly Ile Gln Asn Ile Leu Glu IlePro Leu Thr 50 55 60 Asp His Glu Glu Glu Ser Met Gln Lys Ser Ala Ser GlnLeu Lys Lys 65 70 75 80 Val Leu Thr Asp Ala Phe Ala Lys Asn Asp Ile GluThr Arg Gln 85 90 95 109 323 PRT Lactobacillus rhamnosus 109 Met Leu ThrLys Arg Gln Leu Leu Val Leu Lys Glu Ile Ile Arg Leu 1 5 10 15 Phe ThrGlu Ser Gly Gln Pro Val Gly Ser Lys Thr Leu Met Gln Glu 20 25 30 Leu ProVal His Val Ser Ser Ala Thr Ile Arg Asn Asp Met Ala Ser 35 40 45 Leu GluAsp Ala Gly Leu Ile Thr Lys Thr His Ser Ser Ser Gly Arg 50 55 60 Val ProSer Thr Gln Gly Tyr Arg Tyr Tyr Leu Asp His Leu Val Glu 65 70 75 80 ProVal Arg Val Ser His Arg Glu Leu Ala Thr Ile Lys Gln Ala Phe 85 90 95 GlyGln Arg Tyr Asn Lys Met Asp Glu Ile Val Ala Gln Ser Ala Gln 100 105 110Ile Leu Ser Asn Leu Thr Ser Tyr Thr Ala Ile Ser Leu Gly Pro Glu 115 120125 Val Asn Asn Ile Lys Leu Thr Gly Phe Arg Leu Val Pro Leu Gly Asn 130135 140 His Gln Val Met Ala Ile Leu Val Thr Asn Asn Gly Asn Val Glu Asn145 150 155 160 Gln Val Phe Thr Val Pro Glu Ser Ile Ser Ser Asp Glu LeuGlu Lys 165 170 175 Ala Ile Arg Ile Val Asn Asp Gln Leu Val Gly Leu ProLeu Ile Gln 180 185 190 Val Ala Gln Arg Leu Lys Thr Asp Val Pro Ser MetLeu Met Gln Tyr 195 200 205 Leu Thr Ser Pro Glu Gly Phe Leu Asp Ile PheGly Asn Val Leu Lys 210 215 220 Ser Ala Ala Ser Glu Arg Phe Tyr Val GlyGly Arg Leu Asn Leu Met 225 230 235 240 Asp Tyr Leu Gly Asp Ser Asp IleHis Glu Leu Lys Lys Ile Met Ser 245 250 255 Leu Ile Asp Ala Asp His GlyAsp Leu Thr Glu Leu Leu Gly Gly Pro 260 265 270 Val Arg Gln Thr Pro ValThr Val Arg Leu Gly Pro Glu Leu Lys Pro 275 280 285 Ile Asp Leu Ala AsnLeu Lys Leu Ile Thr Ala Ser Tyr Asp Val Gly 290 295 300 Asp His Gly ThrGly Met Ile Ala Leu Leu Gly Pro Thr Gln Met Pro 305 310 315 320 Phe SerLys 110 306 PRT Lactobacillus rhamnosus 110 Met Phe Glu His Gly Phe IleGlu Val His Asp Ala Asn Gln Asn Asn 1 5 10 15 Leu Gln His Val Asn ValLys Ile Pro Lys Asp Ala Ile Thr Val Phe 20 25 30 Val Gly Arg Ser Gly SerGly Lys Ser Ser Leu Val Phe Asp Thr Ile 35 40 45 Ala Ala Glu Ser Arg ArgGlu Leu Asn Glu Thr Phe Pro Ser Phe Thr 50 55 60 Gln Gln Tyr Leu Pro LysTyr Gly Gln Pro Asp Val Gly Ser Ile Asp 65 70 75 80 His Leu Pro Val AlaIle Val Val Glu Gln Lys Arg Ile Gly Lys Asn 85 90 95 Ala Arg Ser Thr LeuAla Thr Tyr Thr Gly Ile Tyr Ser Leu Leu Arg 100 105 110 Leu Leu Phe SerArg Ala Gly Lys Pro Phe Ile Gly Tyr Ser Asp Thr 115 120 125 Phe Ser PheAsn Leu Pro Gln Gly Met Cys Pro Thr Cys Gln Gly Leu 130 135 140 Gly TyrVal Asp Asp Ile Asp Val Ser Lys Leu Ile Asp Pro Asn Lys 145 150 155 160Ser Leu Asn Gln Glu Ala Ile Thr Phe Val Ser Phe Gly Pro Asp Thr 165 170175 Trp Arg Trp Arg Arg Tyr Ala Tyr Ser Gly Leu Phe Asp Asn Asp Lys 180185 190 Pro Leu Arg Asp Tyr Thr Pro Glu Glu Met Lys Leu Leu Leu Tyr Ala195 200 205 Pro Gln Gln Thr Leu Lys His Ala Pro Ala Lys Trp Pro Arg ThrAla 210 215 220 Leu Tyr Glu Gly Val Val Pro Arg Ile Lys Arg Ser Ile IleGly Lys 225 230 235 240 Lys Glu Ala Glu His His Lys Ala Ala Leu Ala GluIle Val Thr Arg 245 250 255 Lys Pro Cys Pro Asp Cys Gln Gly Thr Arg LeuArg Pro Glu Val Leu 260 265 270 Thr Cys Leu Ile Asn Gln Thr Asn Ile AlaGln Val Leu Gln Met Asp 275 280 285 Leu Val Asn Val Arg His Phe Leu LysAsn Ile Gln Val Pro Leu Val 290 295 300 Gln Asp 305 111 253 PRTLactobacillus rhamnosus 111 Met Thr Gln Ser Ala Asp Pro His Ala Pro ProLeu Leu Ala Lys Trp 1 5 10 15 Arg Gln Trp Asp Thr Asp Arg His Lys SerSer Pro Phe Glu His Pro 20 25 30 Asn Pro Glu Val Pro Gly Ala Ser Asp ArgLeu Leu Thr Glu Glu Ile 35 40 45 Ala Gly Ile Phe Ile Leu Gly Thr Asn GlyGlu Ser Tyr Val Leu Ala 50 55 60 Glu Asp Glu Lys Leu Ala Phe Val Glu HisVal Ile Asp Tyr Val His 65 70 75 80 Gly Arg Thr Lys Val Leu Val Gly ThrGly Leu Asn Gly Thr Ala Glu 85 90 95 Thr Ile Arg Phe Ser Gln Lys Val AlaSer Leu Lys Pro Asp Ala Ile 100 105 110 Thr Leu Val Ala Pro Ser Phe ValAla Pro Ser Gln Gln Glu Leu Val 115 120 125 Asp His Val Ala Ala Ile IleHis Ala Asp Asp Ile Pro Val Leu Leu 130 135 140 Tyr Asn Met Pro Ala LysThr Gly Ile Asn Ile Glu Pro Ala Ser Leu 145 150 155 160 Lys Gln Leu SerLys Tyr Glu Asn Leu Ile Gly Ile Lys Asp Ser Ser 165 170 175 Gly Lys TrpGlu Asn Phe Asp Gly Tyr Leu Ala Asn Arg Pro Glu Arg 180 185 190 Pro PheSer Val Ile Met Gly Ser Asp Gly Arg Ile Leu Glu Ser Phe 195 200 205 GlnHis Gly Gly Asn Ala Ala Ile Ala Ser Thr Ala Asn Leu Leu Thr 210 215 220Ala Asn Asn Val Ala Leu Tyr Gln Ala Phe Val Asn Asp Asn Ile Glu 225 230235 240 Lys Ala Gln Lys Phe Gln Asp Arg Ile Gln Pro Leu Arg 245 250 112217 PRT Lactobacillus rhamnosus 112 Met Ala Met Ser Pro Ile Asn Ala ProThr Ile Lys Gly Thr Ser Lys 1 5 10 15 Ala Thr Asp Gln Gly Val Asp TrpSer Arg Tyr Gln Gly Asp Asn Gly 20 25 30 Val Phe Gly Tyr Ser Thr Asp LysPhe Gly Ile Ser Gln Ile Gly Gly 35 40 45 Tyr Ser Gly Tyr Gly Thr Tyr GluGln Thr Thr Tyr Lys Thr Gln Val 50 55 60 Ala Ser Leu Ile Ala Ala Gly LysArg Ala His Thr Tyr Ile Trp Trp 65 70 75 80 Gln Asn Ile Asp Asn Thr AsnPhe Ala Lys Gln Val Leu Asp His Phe 85 90 95 Leu Pro Glu Ile Gln Thr ProLys Gly Ser Ile Val Ala Leu Asp Tyr 100 105 110 Glu Ala Gly Ser Thr AsnThr Ala Thr Leu Leu Trp Ala Leu Asp Tyr 115 120 125 Ile Arg Asp Ala GlyTyr Thr Pro Met Leu Tyr Gly Tyr Lys Ser Phe 130 135 140 Leu Met Ser HisIle Asp Leu Ser Gln Ile Ala Ser Arg Tyr Gln Leu 145 150 155 160 Trp LeuAla Glu Tyr Pro Asp Tyr Asn Val Thr Thr Val Pro Asn Tyr 165 170 175 GlyTyr Phe Pro Ser Phe Asp Asn Val Gly Ile Phe Gln Phe Thr Ser 180 185 190Thr Tyr Arg Ala Gly Gly Leu Asp Gly Asn Val Asp Arg Ser Pro Arg 195 200205 Thr Leu Thr Lys Pro Arg Cys Cys Gln 210 215 113 282 PRTLactobacillus rhamnosus 113 Met Ala Thr Ser His Phe Lys Ala Ser Lys GlnLeu Cys Tyr Tyr Leu 1 5 10 15 Leu Gly Val Leu Gly Ile Ala Val Val PheGly Leu Gly Leu Phe Gly 20 25 30 Gly Tyr Phe Val Ser Ile Ile Asp Ala ThrPro Ile Pro Thr Glu Thr 35 40 45 Ala Met Lys Ala Thr Leu Ser Asn Thr SerArg Thr Ser Ser Met Tyr 50 55 60 Phe Ala His Asn Val Lys Leu Ser Asp ValLys Ser Asp Leu Tyr Ser 65 70 75 80 Thr Lys Val Asn Leu Asn Glu Met SerPro Trp Leu Thr Lys Ala Ile 85 90 95 Ile Ala Thr Glu Asp Glu Asp Phe TyrArg His Asn Gly Ile Val Pro 100 105 110 Lys Ala Val Ile Arg Ala Phe PheSer Asp Leu Thr Gly Met Gly Ser 115 120 125 Gln Thr Gly Gly Ser Thr LeuThr Gln Gln Val Val Lys Met Met Phe 130 135 140 Leu Asn Ser Glu Thr ThrPhe Lys Arg Lys Ala Ala Glu Ile Met Leu 145 150 155 160 Ala Arg Arg LeuAsn Asn His Phe Ser Lys Asn Thr Ile Leu Ala Thr 165 170 175 Tyr Leu AsnVal Ala Thr Leu Gly Arg Asn Asn Lys Gly Gln Asn Ile 180 185 190 Ala GlyVal Glu Ala Ala Ala Gln Gly Leu Phe Gly Val Ser Ala Lys 195 200 205 GluVal Asn Leu Pro Glu Ala Ala Phe Ile Ala Gly Leu Pro Gln Ser 210 215 220Pro Phe Val Tyr Thr Pro Tyr Thr Ala Asp Gly Lys Leu Lys Thr Ser 225 230235 240 Leu Lys Ala Gly Ile Asn Arg Gln Gln Thr Val Leu Phe Arg Met Tyr245 250 255 Arg Ala Gly Val Ile Ser His Arg Gln Tyr Val Ala Ala Lys SerPhe 260 265 270 Asp Pro Leu Val Ser Thr Cys Arg Arg Ala 275 280 114 418PRT Lactobacillus rhamnosus 114 Met Thr Thr Val Gly His Ile Arg Asn GluLeu Leu Ala Thr Phe Arg 1 5 10 15 Lys Asn Pro Asn Ile Asp Tyr Ser ValGln Thr Leu Ser Arg Ala Leu 20 25 30 Lys Leu Ser Glu Gly Gly Asp Phe LysVal Leu Val Gln Ala Leu Asn 35 40 45 Gly Met Glu Asn Asp Asn Leu Ile HisAla Asn His Glu Gly Arg Tyr 50 55 60 Ala Leu Gly Gly Ala Pro Lys Val LeuThr Gly Thr Phe Arg Gly Asn 65 70 75 80 Glu Lys Gly Phe Gly Phe Val AlaVal Glu Gly Leu Asp Asn Asp Val 85 90 95 Tyr Val Pro Ala Met Asn Thr AspPhe Ala Leu Asp Gly Asp Thr Val 100 105 110 Glu Val Arg Ile Val Arg GluAla Arg Pro Asn Asp Ser Arg Gly Pro 115 120 125 Glu Gly Glu Ile Thr LysIle Val Gln Arg Ser Leu Thr Thr Leu Val 130 135 140 Gly Glu Phe Lys ProPhe Ser Asp Lys Asp Arg Ala Lys Ser Gly Phe 145 150 155 160 Ile Gly MetVal Val Ser His Glu Lys Lys Leu Lys Asn Phe Pro Val 165 170 175 Tyr ValLys Asp Thr Gly Asn Ile Pro Gln Leu Gly Asp Met Thr Val 180 185 190 ThrGlu Ile Thr Glu Phe Pro Thr Glu Tyr His Pro Lys Leu Met Tyr 195 200 205Gly Ile Val Val Glu Thr Leu Gly Asn Lys Asn Asp Pro Gly Val Asp 210 215220 Ile Met Ser Leu Val Met Gln Asn His Ile Lys Thr Glu Phe Pro Asp 225230 235 240 Glu Val Met Asp Gln Thr Asn Ala Ile Pro Asp His Val Thr ProGlu 245 250 255 Glu Arg Val Gly Arg Lys Asp Ile Thr Asp Gln Ala Val ValThr Ile 260 265 270 Asp Gly Asp Asp Ser Lys Asp Phe Asp Asp Ala Val ValVal Trp Lys 275 280 285 Leu Pro Asn Gly Asn Phe His Leu Gly Val His IleAla Asp Val Ser 290 295 300 His Tyr Val Thr Glu Gly Ser Ala Leu Asp GlnGlu Ala Phe Asp Arg 305 310 315 320 Gly Thr Ser Thr Tyr Leu Val Asp ArgVal Ile Pro Met Leu Pro Phe 325 330 335 Arg Leu Ser Asn Gly Ile Cys SerLeu Asn Pro Gly Val Asp Arg Leu 340 345 350 Ala Met Ser Cys Asp Met GluIle Asp His Asp Gly His Val Val Asn 355 360 365 His Glu Ile Tyr Gln SerVal Ile Lys Ser His Ala Arg Met Thr Tyr 370 375 380 Asn Asn Val Asn LysIle Val Thr Asp Pro Asp Pro Glu Val Met Ala 385 390 395 400 Glu Tyr GlnGlu Leu Val Pro Met Phe Glu Asp Met Val Glu Leu His 405 410 415 Gln Ile115 295 PRT Lactobacillus rhamnosus 115 Tyr Glu Asn Glu Ile Ile Ile ThrIle Arg Ala Gly Arg Lys Asn His 1 5 10 15 Pro Leu Leu Leu Ser Ala AsnPro Gln Tyr Ala Arg Val Gln Ile Thr 20 25 30 His Ile Pro Phe Thr Asn ProAsp Val Pro Ala Thr Phe Thr Met Thr 35 40 45 Leu Arg Lys Tyr Phe Asn AlaAla Thr Leu Thr Glu Ile His Gln Val 50 55 60 Gln Asn Asp Arg Val Leu HisPhe Glu Phe Ser Thr Arg Asp Glu Leu 65 70 75 80 Gly Asp Glu Leu Gly LeuArg Leu Ile Ile Glu Met Met Gly Arg His 85 90 95 Ser Asn Ile Phe Leu ValSer Lys Arg Thr Gly Lys Ile Ile Asp Leu 100 105 110 Ile Arg His Val SerAla Asp Gln Asn Arg Tyr Arg Pro Leu Met Pro 115 120 125 Gly Ala Pro TyrVal Glu Pro Pro Lys Gln Asp Lys Val Asp Pro Phe 130 135 140 His Asp SerGlu Arg Ile Tyr His Glu Leu Glu Arg Gln Val Thr Pro 145 150 155 160 SerLeu Ser Arg Ala Ala Leu Leu Gln Gln His Tyr Gln Gly Leu Ala 165 170 175Lys Asp Ser Ala Ala Glu Leu Ala Leu Arg Leu Asn Gln Gly Asp Ala 180 185190 Gly Trp Asp Ser Phe Phe Ala Ala Leu Ala Thr Pro Glu Pro Thr Ile 195200 205 Thr Thr Gln Gly Lys Lys Ala Val Phe Thr Ala Ile Pro Tyr Gln Ser210 215 220 Leu Thr Gly Glu Gln Gln His Phe Pro Thr Leu Ser Ala Met LeuAsp 225 230 235 240 Ala Tyr Tyr Ala Gln Lys Ala Glu His Asp Arg Val LeuGln Gln Gly 245 250 255 Gly Asn Leu Ile His Val Ile Lys Asn Val Ile AspLys Asp Arg Lys 260 265 270 Lys Gln Arg Lys Leu Lys Arg Thr Leu Glu GluThr Glu Lys Ala Asp 275 280 285 Asp Tyr Arg Ile Arg Phe Lys 290 295 116231 PRT Lactobacillus rhamnosus 116 Met Trp His Leu Pro Ile Thr Gly TyrThr Phe Tyr Arg Ser Asp Met 1 5 10 15 Met Pro Val Lys Lys Thr Asn AlaVal Asn Leu Ser Leu Phe Ile Leu 20 25 30 Leu Leu Thr Leu Glu Ile Ser PheSer His Ala Val Ser Leu Asn Val 35 40 45 Ala Leu Ile Gly Leu Ala Ser GlyPhe Leu Ile Trp Arg Arg Ala Phe 50 55 60 Lys Ser Leu Val Val Leu Ala LeuLeu Pro Leu Ile Pro Ala Ala Ser 65 70 75 80 Thr Tyr Trp Ala Ile Thr LeuHis Gly Thr Asp Thr Thr Tyr Ala Leu 85 90 95 Leu Leu Trp Val Arg Thr TyrAla Phe Thr Ala Leu Gly Leu Val Phe 100 105 110 Leu Ile Gly Val Asp LeuGlu Thr Leu Leu Leu Trp Leu Glu Gln His 115 120 125 Lys Leu Ser Pro AsnPhe Val Tyr Gly Leu Leu Val Val Ile His Ala 130 135 140 Leu Pro Gln IleMet His Glu Val Ala Ala Ile Arg Glu Ala Ser Leu 145 150 155 160 Leu ArgGly Gln Lys Leu His Ala Trp Ser Pro Met Ile Tyr Val Lys 165 170 175 ValIle Phe Val Ala Met Ser Trp Gln Asp Gln Tyr Val Lys Ala Met 180 185 190Tyr Ala His Gly Tyr Thr Glu Gly Ala Ala Arg Thr Val His Gln Thr 195 200205 Ile Arg Ser Ser Trp Arg Gly Leu Ile Ala Met Val Gly Gly Phe Val 210215 220 Leu Leu Asn Leu Ile Asp Arg 225 230 117 225 PRT Lactobacillusrhamnosus 117 Met Met Ala Lys Ile Leu Ile Val Glu Asp His Arg Ile SerArg His 1 5 10 15 Leu Leu Lys Asp Val Leu Thr Pro Thr Tyr Thr Val ThrGln Ala Tyr 20 25 30 Asp Gly Ile Gln Ala Leu Thr Ala Phe His Arg Glu GlnPro Asp Leu 35 40 45 Ile Ile Leu Asp Leu Met Leu Pro Asn Val Thr Gly GluSer Val Leu 50 55 60 Thr Thr Ile Arg Lys Thr Ser Gln Val Pro Val Leu ValLeu Thr Ala 65 70 75 80 Ile Gln Glu Lys Ala Lys Thr Val Ala Leu Leu GlnGln Gly Ala Asn 85 90 95 Asp Tyr Leu Thr Lys Pro Phe Asp Ile Asp Glu LeuLeu Ala Arg Ile 100 105 110 Gln Val Gln Leu Arg Gln Val Ser Gly Gln ProIle Thr Thr Asn Asp 115 120 125 Gln Leu Lys Val Gly Glu Ile Gln Leu AspPro Lys Arg His Val Val 130 135 140 Thr Val Asn Gln Gln Thr Leu Thr LeuPro Lys Lys Glu Tyr Asp Met 145 150 155 160 Leu Ala Leu Met Met Arg AspPro His Gln Val Phe Asp Lys Ser Gln 165 170 175 Leu Tyr Glu His Val TrpGly Glu Pro Phe Leu Asn Ala Asp Asn Thr 180 185 190 Leu Asn Val His IleSer Asn Leu Arg Thr Lys Ile Asn Glu Leu Ala 195 200 205 His Asp Pro LysTyr Ile Ile Ser Ile Trp Gly Ile Gly Val Arg Leu 210 215 220 Ile 225 118259 PRT Lactobacillus rhamnosus 118 Arg Phe Ala Ser Val Pro Gln Asp ProAsp Asn Leu Ala Gly Ile Asp 1 5 10 15 Ser Asn Arg Ile Ala Lys Tyr GlnGlu Ala Phe Ala Lys Ala Tyr Lys 20 25 30 Arg Leu Met Glu Ala Ile Ser SerMet Ser Ile Ser Trp Thr Ile Ile 35 40 45 Gly Ala Ala Ser Pro Arg Trp AlaGln Lys Val Phe Pro Asp Ala Ala 50 55 60 Thr Pro Glu Glu Ala Thr Glu LeuLeu Trp Glu Ala Ile Phe Lys Thr 65 70 75 80 Thr Arg Ile Asp Gln Pro AspPro Glu Ala Ala Trp Lys Ala His Asp 85 90 95 Gln Lys Leu Arg Glu Lys AlaAla Trp Leu Asn Asn Glu Gln Phe Asp 100 105 110 Gln Leu His Tyr Met AlaPro Gly Thr Asp Leu Val Val Gly Leu Pro 115 120 125 Lys Asn His Ile TrpGlu Gly Ala Gly Ala Phe Asn Pro Arg Gly Glu 130 135 140 Glu Phe Met AlaAsn Met Pro Thr Glu Glu Val Phe Thr Ala Pro Asp 145 150 155 160 Phe ArgArg Ile Asp Gly Thr Val Ala Ser Thr Lys Pro Leu Ser Tyr 165 170 175 GlyGly Asn Ile Leu Glu Asp Met His Phe Thr Phe Lys Asp Gly Gln 180 185 190Ile Val Glu Ala His Ala Lys Gln Gly Asp Asp Val Leu Gln Asn Leu 195 200205 Leu Lys Thr Pro Gly Ala Arg Ser Leu Gly Glu Val Ser Leu Val Pro 210215 220 Asp Pro Ser Ser Ile Ser Gln Ser Gly Leu Ile Phe Phe Asn Thr Leu225 230 235 240 Val Asp Glu Asn Ala Ser Asp His Met Ala Leu Gly Gln AlaTyr Pro 245 250 255 Phe Ser Val 119 438 PRT Lactobacillus rhamnosus 119Val Lys Arg Ala Leu Leu Ser Val Ser Asp Lys Thr Gly Leu Val Pro 1 5 1015 Phe Ala Lys Gly Leu Val Glu Arg Gly Phe Glu Leu Ile Ser Thr Gly 20 2530 Gly Thr His Arg Ala Leu Ala Glu Ala Gly Val Ala Val Thr Gly Val 35 4045 Glu Ala Val Thr Gly Phe Pro Glu Met Leu Asp Gly Arg Val Lys Thr 50 5560 Leu His Pro Lys Ile His Ala Gly Ile Leu Ala Arg Arg Asp Asp Pro 65 7075 80 Ala His Met Gln Ala Leu Ala Asp His Asp Ile Gln Pro Ile Asp Val 8590 95 Val Cys Val Asn Leu Tyr Pro Phe Ala Ala Thr Ile Gln Arg Ala Gly100 105 110 Val Thr Arg Ala Glu Ala Ile Glu Gln Ile Asp Ile Gly Gly ProSer 115 120 125 Ala Leu Arg Ala Ala Ala Lys Asn Ser Asp Ser Val Trp AlaVal Val 130 135 140 Asp Pro Ala Asp Tyr Ala Asp Val Leu Ala Gly Leu AspGln Asn Asp 145 150 155 160 Ala Asp Leu Arg Gln Arg Leu Ala Ala Lys ValPhe Ser Ala Thr Ala 165 170 175 Ala Tyr Asp Ala Gln Ile Ala His Tyr LeuAsp Pro Glu Pro Phe Pro 180 185 190 Glu Gln Phe Thr Pro Thr Tyr His LysArg Gln Asp Leu Arg Tyr Gly 195 200 205 Glu Asn Ser His Gln Gln Ala AlaPhe Tyr Val Glu Pro Asn Pro Asp 210 215 220 Pro Thr Ser Leu Ala Ala AlaLys Gln Leu His Gly Lys Glu Leu Ser 225 230 235 240 Tyr Asn Asn Ile LysAsp Ala Asp Ala Ala Leu Ala Met Leu Arg Glu 245 250 255 Phe Lys Gln ProAla Ala Val Ala Val Lys His Met Asn Pro Cys Gly 260 265 270 Ile Gly LeuGly Asp Thr Leu Glu Ala Ala Trp Asp Lys Ala Tyr Ala 275 280 285 Ala AspPro Met Ser Ile Phe Gly Gly Ile Ile Ala Leu Asn Arg Arg 290 295 300 ValAsp Leu Ala Thr Ala Glu Lys Met His Lys Leu Phe Leu Glu Ile 305 310 315320 Ile Met Ala Pro Ala Phe Asp Asp Asp Ala Tyr Glu Ile Leu Ala Lys 325330 335 Lys Lys Asn Val Arg Leu Leu Thr Ile Asn Thr Ala Asp Thr Pro Glu340 345 350 Glu Leu Gly Thr Glu Thr Thr Ser Ile Tyr Gly Gly Leu Leu IleGln 355 360 365 Thr Arg Asp Asp Lys Ala Glu Thr Pro Ala Asp Met Thr ValVal Thr 370 375 380 Glu Val Lys Pro Thr Glu Ala Gln Leu Lys Ala Leu AlaPhe Ala Gln 385 390 395 400 Thr Val Val Lys His Val Lys Ser Asn Ala IleVal Val Ala Gln Ala 405 410 415 Asp Gln Thr Leu Gly Ile Gly Ala Gly GlnMet Asn Arg Ile Gly Ser 420 425 430 Val Glu Leu Ala Leu Thr 435 120 279PRT Lactobacillus rhamnosus 120 Met Val Lys Arg Asn Pro Asn Gly Thr ArgPhe Ile Thr Leu Pro Asn 1 5 10 15 Gly Tyr His Leu Trp Thr Gln Thr LeuAla Ala Ala Asp Ser Leu Leu 20 25 30 Thr Leu His Gly Gly Pro Gly Gly ThrAsn Glu Val Phe Glu Asn Phe 35 40 45 Ala Thr Glu Leu Ala Ser Phe Gly ValArg Val Ser Arg Tyr Asp Gln 50 55 60 Leu Gly Ser Phe Phe Ser Asp Gln ProAsp Phe Ser Asp Pro Ala Asn 65 70 75 80 Gln Lys Arg Phe Leu Asn Ile AlaTyr Tyr Val Asp Glu Val Glu Asn 85 90 95 Val Arg Gln Gln Leu Gly Leu AspHis Phe Tyr Leu Leu Gly Gln Ser 100 105 110 Trp Gly Gly Val Leu Ala IleGlu Tyr Gly Leu Lys Tyr Ser Gln His 115 120 125 Leu Lys Gly Leu Ile LeuSer Ser Met Ile Asp Asn Leu Asp Glu Tyr 130 135 140 Leu Val Asn Ile AsnLys Ile Arg Glu Thr Met Phe Ser Ser Asp Asp 145 150 155 160 Val Ala TyrMet Gln Arg Ile Glu Ala Gln His Ala Phe Thr Asp Ala 165 170 175 Lys TyrGln Gln Leu Val Arg Glu Leu Gly Glu Gln Tyr Leu His His 180 185 190 AlaLys Asp Pro Gln Pro Arg His Leu Ile Ser Thr Leu Ala Thr Pro 195 200 205Val Tyr His His Phe Gln Gly Asp Asn Glu Phe Val Met Val Gly Ala 210 215220 Leu Arg Asp Trp Asp Arg Arg Ala Asp Ile His Arg Leu Thr Met Pro 225230 235 240 Thr Tyr Leu Thr Phe Gly Gly His Glu Thr Met Pro Leu Ser AlaAla 245 250 255 Lys Arg Met Ala Arg Thr Ile Pro Asn Ala Thr Leu His ValThr Pro 260 265 270 Asn Ala Gly His Gly Gln Met 275 121 317 PRTLactobacillus rhamnosus 121 Met Ser Arg Arg Tyr Arg Gln Phe Asp Ala AsnArg Ala Gly Ser Arg 1 5 10 15 Ser Arg Gly Gly Leu Asn Leu Ile Ser LeuGly Ile Tyr Glu Lys Ala 20 25 30 Leu Pro Arg Thr Glu Ser Trp Val Glu ArgLeu Lys Met Val Arg Asp 35 40 45 Leu Gly Phe Asn Phe Leu Glu Leu Ser ValAsp Glu Ser Asp Glu Arg 50 55 60 Leu Ala Arg Leu Asp Trp Thr Ala Ala LysArg Ala Lys Val Arg Asp 65 70 75 80 Ala Cys Trp Gln Thr Gly Val Arg IleHis Thr Leu Met Leu Ser Gly 85 90 95 His Arg Arg Phe Pro Leu Gly Ser AlaAsp Pro Ala Ile Arg Glu Lys 100 105 110 Ser Leu Thr Met Leu Cys Lys AlaIle Asp Leu Ala Ser Asp Leu Gly 115 120 125 Val Arg Asn Val Gln Leu AlaGly Tyr Asp Val Tyr Tyr Glu Pro Lys 130 135 140 Thr Leu Ala Ser Arg GluTyr Phe Ile Glu Asn Leu Lys Arg Gly Val 145 150 155 160 Ala Tyr Ala AlaAla Lys Glu Val Met Leu Ala Ile Glu Thr Met Asp 165 170 175 Asp Pro PheLeu Asn Ser Leu Ser Lys Ile Lys Thr Ile Lys Asp Glu 180 185 190 Ile ProSer Pro Trp Leu Gln Ala Tyr Pro Asp Leu Gly Asn Leu Ser 195 200 205 AlaTrp Pro Glu Asn Asn Val Gly Arg Glu Leu Glu Leu Gly Ile Ala 210 215 220Asn Ile Val Ser Val His Leu Lys Asp Thr Gln Ala Val Thr Val Lys 225 230235 240 Ser Lys Gly Gln Phe Arg Asp Val Pro Phe Gly Ala Gly Val Val Asp245 250 255 Phe Ser Gly Cys Leu Arg Thr Leu Lys Arg Leu Asp Tyr Ser GlyAla 260 265 270 Phe Thr Ile Glu Met Trp Thr Glu Lys Ala Ala Asp Pro IleGln Glu 275 280 285 Val Lys Gln Ala Lys Asp Phe Phe Asp Pro Leu Phe ValGln Ala Gly 290 295 300 Phe Val Gln Glu Pro Val Ala Lys Thr Asn Val ProSer 305 310 315 122 310 PRT Lactobacillus rhamnosus 122 Met Thr Asp ProIle Ala Phe Leu Gln Lys Leu Ile Gln Ile Asp Ser 1 5 10 15 Ala Asn GlyAsn Glu Leu Ala Val Ala Arg Val Leu Gln Ala Glu Leu 20 25 30 Glu Ala AlaAsp Ile Pro Thr Lys Leu Ile Pro Tyr Lys Asp Asp Arg 35 40 45 Val Asn LeuVal Ala Gln Leu Asn His Gly Asp Arg Val Leu Gly Phe 50 55 60 Thr Gly HisGlu Asp Val Val Ser Pro Gly Asp Glu Asn Ala Trp Thr 65 70 75 80 Tyr ProPro Phe Ser Gly Lys Ile Val Asn Asn Thr Met Tyr Gly Arg 85 90 95 Gly ThrAsp Asp Met Lys Ser Gly Leu Ala Ala Met Thr Leu Ala Leu 100 105 110 IleHis Leu Lys Gln Ser Gly Phe Ala His Pro Leu Arg Phe Met Ala 115 120 125Thr Val Gly Glu Glu Phe Gly Ala Met Gly Ala Arg Gln Leu Thr Glu 130 135140 Gln Gly Tyr Ala Asp Asp Leu Thr Gly Leu Val Val Gly Glu Pro Thr 145150 155 160 Asn Lys Leu Leu Lys Tyr Ala His Gly Gly Thr Val Asn Tyr GluIle 165 170 175 Asp Ser Glu Gly Val Ser Val His Ser Ser Arg Pro Glu LysGly Val 180 185 190 Asn Ala Ile Glu Gly Leu Val Ala Phe Ser Thr Pro GluPro His Ala 195 200 205 Phe Asp Gln Ala Pro Asp Asp Pro Asp Leu Gly ProPhe Arg His Ser 210 215 220 Ile Thr Val Ile Lys Gly Gly Asp Gln Val AsnThr Ile Pro Ala His 225 230 235 240 Ala Tyr Leu Arg Gly Asn Leu Arg ProThr Pro Ala Ala Asn Ile Glu 245 250 255 Leu Val Val Gly Leu Leu Glu LysLeu Val Asp Gln Ala Asn Lys Ala 260 265 270 Thr Ala Ala Asn Leu Thr LeuAsn Val Leu His Arg Phe Leu Pro Val 275 280 285 His Ser Asp Lys Asn GlyHis Leu Val Thr Thr Ala Asn Glu Ala Ile 290 295 300 Ala Ala Val Thr GlyLys 305 310 123 246 PRT Lactobacillus rhamnosus 123 Met Lys Ile Asp IleAsp Lys Thr Ser Met Ile Pro Val Tyr Glu Gln 1 5 10 15 Ile Ala Asn SerLeu Arg Asp Met Met Tyr Gly Gly Ser Leu Gln Asp 20 25 30 Gly Asp Arg LeuAsp Ser Glu Gln Lys Met Cys Arg Asn Leu Asn Val 35 40 45 Ser Arg Gly ThrVal Arg Lys Ala Ile Asp Ile Leu Leu Lys Glu Gly 50 55 60 Met Val Lys LysIle His Gly Lys Gly Thr Phe Val Ser Asn Pro Asn 65 70 75 80 Val Glu TyrSer Leu Asn Asp Gln Leu Met Ser Phe Ala Glu Ser Leu 85 90 95 Asp Asn GlnHis Leu Ser Tyr Thr Thr Gln Val Ile Gln Gln Glu Leu 100 105 110 Arg ProAla Thr Ala Lys Ile Ala Asp Met Leu Lys Ile Pro Ile Asp 115 120 125 SerGln Tyr Leu Tyr Leu Glu Arg Leu Arg Ser Val Ala Asp Asp Lys 130 135 140Leu Met Leu Ile Glu Asn Arg Ile Asn Ile Thr Leu Cys Pro Gly Ile 145 150155 160 Glu Lys Val Asn Phe Asn Asn Ile Ser Leu Phe Asn Glu Ile Glu Glu165 170 175 Leu Ala Lys Arg Lys Ile Ser Phe Ala Arg Ser Thr Tyr Glu AlaLeu 180 185 190 Thr Ile Gly Thr Glu Arg Gly Lys Leu Leu Glu Leu Pro SerSer Thr 195 200 205 Pro Ala Leu Lys Met Gln Gln Thr Val Tyr Leu Ser GluLys Glu Pro 210 215 220 Val Glu Tyr Gly Ser Val Trp Leu Lys Gly Asn LysTyr Phe Leu Thr 225 230 235 240 Thr Thr Leu Gln Arg Arg 245 124 520 PRTLactobacillus rhamnosus 124 Leu Val Asp Pro Phe Leu Phe Thr Pro Gln LeuThr Ile Glu Glu Val 1 5 10 15 Lys Lys Ala Gly Trp Ala Tyr Pro Val PheGly Tyr Leu Asp His Glu 20 25 30 Asp Pro Phe Ala Lys Leu Ala Ser His IleLys Thr Val Asn Pro Asn 35 40 45 Pro Thr Lys Trp Ala Ile Glu Lys Asp AsnLeu Ala Val Phe Lys Phe 50 55 60 Glu Ala Ile Met Lys Gln Phe Pro Asp AlaThr Phe Pro Ile Asp Ala 65 70 75 80 Ser Arg Phe Ile Glu Lys Gln Arg LeuIle Lys Thr Ala Ser Glu Ile 85 90 95 Lys Gln Met Glu Ala Ala Gly Ala GlnAla Asp Arg Ala Phe Gln Ala 100 105 110 Gly Phe Asn Ala Ile Lys Ala GlyAla Thr Glu Gln Glu Val Ala Ala 115 120 125 Glu Ile Asp Tyr Ala Met MetLys Glu Gly Val Met His Met Ser Phe 130 135 140 Gly Thr Ile Val Gln AlaGly Val Asp Ala Ala Asn Pro His Gly Glu 145 150 155 160 Pro Met Gly ThrLys Leu Ala Pro Asn Glu Leu Val Leu Phe Asp Leu 165 170 175 Gly Thr AspAsn His Gly Tyr Met Ser Asp Ala Thr Arg Thr Val Ala 180 185 190 Phe GlyGln Val Thr Gly Lys Pro Arg Glu Ile Phe Asp Ile Cys Leu 195 200 205 GluAla Asn Leu Thr Ala Met Asp Ala Val Lys Pro Gly Leu Lys Ala 210 215 220Ser Glu Leu Asp Lys Ile Ala Arg Asp Ile Ile Thr Lys Ala Gly Tyr 225 230235 240 Gly Glu Tyr Phe Asn His Arg Leu Gly His Gly Ile Gly Met Ser Thr245 250 255 His Glu Phe Pro Ser Ile Met Glu Gly Asn Asp Met Ile Val GlyGlu 260 265 270 Asp Phe Gly Met Arg Val Ser Val Leu Ala Ser Ser Ser SerGly Asn 275 280 285 Ala Thr Tyr Ile Glu Thr Pro Gly His Lys Val Leu ValAsp Ala Gly 290 295 300 Leu Ser Gly Lys Lys Ile Glu Ala Leu Met Lys SerIle Gly Arg Asp 305 310 315 320 Leu Thr Asp Val Asp Ser Val Phe Ile ThrHis Glu His Ser Asp His 325 330 335 Val Arg Gly Val Gly Val Leu Ala ArgArg Tyr Pro Gln Leu Asn Val 340 345 350 Tyr Ala Asn Ala Lys Thr Phe AlaAla Leu Pro Lys Ser Val Gly Lys 355 360 365 Ile Pro Glu Ala Gln Leu ArgLeu Phe Asp Met Gly Thr Thr Leu Thr 370 375 380 Leu Gly Asp Leu Asp ValGlu Ser Phe Gly Val Ser His Asp Ala Ala 385 390 395 400 Ala Pro Gln PheTyr Gln Phe His His Asp Gly Lys Ala Phe Thr Ile 405 410 415 Leu Thr AspThr Gly Tyr Val Ser Asp Arg Val Ala Gly Thr Ile Arg 420 425 430 Asp AlaAsp Ala Tyr Val Met Glu Cys Asn His Asp Leu Glu Met Leu 435 440 445 ArgThr Gly Pro Tyr Pro Trp Pro Leu Lys Gln Arg Ile Leu Ser Asp 450 455 460Gln Gly His Leu Ser Asn Glu Asp Gly Ala Asp Ala Leu Met Asp Val 465 470475 480 Ile Gly Leu Arg Thr Lys Arg Ile Tyr Leu Gly His Leu Ser Pro His485 490 495 Asn Asn Asn Lys Ala Thr Gly Ala Phe Asn Arg Gly Val Val ValGly 500 505 510 Thr Thr Arg Ser Gly Gly Gly Ser 515 520

We claim:
 1. An isolated polynucleotide comprising a sequence selectedfrom the group consisting of: SEQ ID NO: 1, 5, 6, 8-10, 12-23, 25-31 and33-62.
 2. An isolated polynucleotide comprising a sequence selected fromthe group consisting of: (a) complements of SEQ ID NO: 1, 5, 6, 8-10,12-23, 25-31 and 33-62; (b) reverse complements of SEQ ID NO: 1, 5, 6,8-10, 12-23, 25-31 and 33-62; and (c) reverse sequences of SEQ ID NO: 1,5, 6, 8-10, 12-23, 25-31 and 33-62; and (d) sequences that aredegeneratively equivalent to a sequence of SEQ ID NO: 1, 5, 6, 8-10,12-23, 25-31 and 33-62.
 3. An isolated polynucleotide comprising asequence selected from the group consisting of: (a) sequences having atleast 75% identity to a sequence of SEQ ID NO: 1, 5, 6, 8-10, 12-23,25-31 and 33-62; (b) sequences having at least 90% identity to asequence of SEQ ID NO: 1, 5, 6, 8-10, 12-23, 25-31 and 33-62; (c)sequences having at least 95% identity to a sequence of SEQ ID NO: 1, 5,6, 8-10, 12-23, 25-31 and 33-62; and (d) sequences that hybridize to asequence of SEQ ID NO: 1, 5, 6, 8-10, 12-23, 25-31 and 33-62 understringent conditions, wherein the polynucleotide encodes a polypeptidehaving substantially the same functional properties as a polypeptideencoded by a sequence of SEQ ID NO: 1, 5, 6, 8-10, 12-23, 25-31 and33-62.
 4. An isolated polynucleotide comprising a sequence selected fromthe group consisting of: (a) sequences that are 200-mers of a sequenceof SEQ ID NO: 1, 5, 6, 8-10, 12-23, 25-31 and 33-62; (b) sequences thatare 100-mers of a sequence of SEQ ID NO: 1, 5, 6, 8-10, 12-23, 25-31 and33-62; (c) sequences that are 40-mers of a sequence of SEQ ID NO: 1, 5,6, 8-10, 12-23, 25-31 and 33-62; and (d) sequences that are 20-mers of asequence of SEQ ID NO: 1, 5, 6, 8-10, 12-23, 25-31 and 33-62.
 5. Anoligonucleotide comprising at least 10 contiguous residues complementaryto contiguous residues of a sequence recited in SEQ ID NO: 1, 5, 6,8-10, 12-23, 25-31 and 33-62.
 6. A genetic construct comprising anisolated polynucleotide of any one of claims 1-4.
 7. A host celltransformed with a genetic construct of claim
 6. 8. A transgenicorganism comprising a host cell according to claim
 7. 9. An isolatedpolypeptide encoded by a polynucleotide of claim
 1. 10. An isolatedpolypeptide comprising an amino acid sequence selected from the groupconsisting of: SEQ ID NO: 63, 67, 68, 70-72, 74-85, 87-93 and 95-124.11. An isolated polypeptide comprising an amino acid sequence selectedfrom the group consisting of: (a) sequences having at least 75% identityto a sequence of SEQ ID NO: 63, 67, 68, 70-72, 74-85, 87-93 and 95-124;(b) sequences having at least 90% identity to a sequence of SEQ ID NO:63, 67, 68, 70-72, 74-85, 87-93 and 95-124; and (c) sequences having atleast 95% identity to a sequence of SEQ ID NO: 63, 67, 68, 70-72, 74-85,87-93 and 95-124, wherein the polypeptide possesses substantially thesame functional properties as a polypeptide of SEQ ID NO: 63, 67, 68,70-72, 74-85, 87-93 and 95-124.
 12. An isolated polynucleotide encodinga polypeptide of claim
 7. 13. An isolated polypeptide comprising atleast a functional portion of a polypeptide comprising an amino acidsequence selected from the group consisting of: (a) SEQ ID NO: 63, 67,68, 70-72, 74-85, 87-93 and 95-124; (b) sequences having at least 75%identity to a sequence of SEQ ID NO: 63, 67, 68, 70-72, 74-85, 87-93 and95-124; (c) sequences having at least 90% identity to a sequence of SEQID NO: 63, 67, 68, 70-72, 74-85, 87-93 and 95-124; and (d) sequenceshaving at least 95% identity to a sequence of SEQ ID NOS: 63, 67, 68,70-72, 74-85, 87-93 and 95-124.
 14. A composition comprising apolypeptide according to any one of claims 7, 8 and 13 and at least onecomponent selected from the group consisting of: physiologicallyacceptable carriers and immunostimulants.
 15. A composition comprising apolynucleotide according to any one of claims 1-4 and at least onecomponent selected from the group consisting of pharmaceuticallyacceptable carriers and immunostimulants.
 16. A method for treating adisorder in a mammal comprising administering a composition according toclaim
 14. 17. A method for treating a disorder in a mammal comprisingadministering a composition according to claim
 15. 18. A diagnostic kitcomprising at least one oligonucleotide according to claim 5.